Development of a CRISPR interference system for selective gene knockdown in Acidithiobacillus ferrooxidans

2021 ◽  
Author(s):  
Shohei Yamada ◽  
Yusuke Suzuki ◽  
Atsushi Kouzuma ◽  
Kazuya Watanabe
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Gene Knockdown ◽  
Crispr Interference ◽  
Interference System

scholarly journals Investigating essential gene function inMycobacterium tuberculosisusing an efficient CRISPR interference system

2016 ◽  
Vol 44 (18) ◽  
pp. e143-e143 ◽  
Author(s):  
Atul K. Singh ◽  
Xavier Carette ◽  
Lakshmi-Prasad Potluri ◽  
Jared D. Sharp ◽  
Ranfei Xu ◽  
...  
Keyword(s):  
Gene Function ◽  
Essential Gene ◽  
Crispr Interference ◽  
Interference System

scholarly journals Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes ameliorates obesity, inflammation, hepatic steatosis, and insulin resistance

2019 ◽  
Vol 29 (9) ◽  
pp. 1442-1452 ◽  
Author(s):  
Jee Young Chung ◽  
Qurrat Ul Ain ◽  
Yoonsung Song ◽  
Seok-Beom Yong ◽  
Yong-Hee Kim
Keyword(s):  
Insulin Resistance ◽  
Hepatic Steatosis ◽  
Targeted Delivery ◽  
Crispr Interference ◽  
White Adipocytes ◽  
Interference System

scholarly journals Engineering CRISPR interference system in Klebsiella pneumoniae for attenuating lactic acid synthesis

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Jingxuan Wang ◽  
Peng Zhao ◽  
Ying Li ◽  
Lida Xu ◽  
Pingfang Tian
Keyword(s):  
Lactic Acid ◽  
Klebsiella Pneumoniae ◽  
Acid Synthesis ◽  
Crispr Interference ◽  
Interference System

scholarly journals CRISPR–Cas12a system in fission yeast for multiplex genomic editing and CRISPR interference

2020 ◽  
Vol 48 (10) ◽  
pp. 5788-5798 ◽  
Author(s):  
Yu Zhao ◽  
Jef D Boeke
Keyword(s):  
Fission Yeast ◽  
Pol Ii ◽  
Endogenous Gene ◽  
Crispr Interference ◽  
Interference System ◽  
Potential Applications ◽  
Short Palindromic Repeat ◽  
Genomic Gene ◽  
Genomic Editing ◽  
Type V

Abstract The CRISPR–Cas12a is a class II, type V clustered regularly interspaced short palindromic repeat (CRISPR) system with both RNase and DNase activity. Compared to the CRISPR–Cas9 system, it recognizes T-rich PAM sequences and has the advantage of multiplex genomic editing. Here, in fission yeast Schizosaccharomyces pombe, we successfully implemented the CRISPR–Cas12a system for versatile genomic editing and manipulation. In addition to the rrk1 promoter, we used new pol II promoters from endogenous coding genes to express crRNA for Cas12a and obtained a much higher editing efficiency. This new design expands the promoter choices for potential applications in fission yeast and other organisms. In addition, we expressed a gRNA array using a strong constitutive pol II promoter. The array transcript is processed by Cas12a itself to release multiple mature crRNAs. With this construct, multiplex genomic editing of up to three loci was achieved from a single yeast transformation. We also built a CRISPR interference system using a DNase-dead Cas12a to significantly repress endogenous gene expression. Our study provides the first CRISPR-Cas12a toolkit for efficient and rapid genomic gene editing and regulation in fission yeast.

Engineering CRISPR interference system to enhance the production of pyrroloquinoline quinone in Klebsiella pneumonia

2020 ◽  
Vol 71 (3) ◽  
pp. 242-250
Author(s):  
Z. Mi ◽  
Z. Sun ◽  
Z. Huang ◽  
P. Zhao ◽  
Q. Li ◽  
...  
Keyword(s):  
Pyrroloquinoline Quinone ◽  
Klebsiella Pneumonia ◽  
Crispr Interference ◽  
Interference System

Establishment and application of multiplexed CRISPR interference system in Bacillus licheniformis

2019 ◽  
Vol 104 (1) ◽  
pp. 391-403 ◽  
Author(s):  
Yangyang Zhan ◽  
Yong Xu ◽  
Pengling Zheng ◽  
Min He ◽  
Shanhu Sun ◽  
...  
Keyword(s):  
Bacillus Licheniformis ◽  
Crispr Interference ◽  
Interference System
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