Recycling of bacterial biomass in a process of l-threonine production by means of a recombinant strain of Escherichia coli

2007 ◽  
Vol 132 (4) ◽  
pp. 431-437 ◽  
Author(s):  
Markus Blaesen ◽  
Karl Friehs ◽  
Erwin Flaschel
Keyword(s):  
Escherichia Coli ◽  
Recombinant Strain ◽  
Bacterial Biomass ◽  
Threonine Production

Study on Optimization of Fermentation Condition for Nitrilase-Producer Escherichia Coli BL21 (DE3)/pET-Nit

2011 ◽  
Vol 175-176 ◽  
pp. 192-196 ◽  
Author(s):  
Li Li Feng ◽  
Jian Fei Zhang ◽  
Hui Luo ◽  
Zheng Li ◽  
Hong Jie Zhang
Keyword(s):  
Escherichia Coli ◽  
Recombinant Strain ◽  
Culture Medium ◽  
Culture Conditions ◽  
Fermentation Condition ◽  
Hydrogen Phosphate ◽  
Strain Bl21 ◽  
Initial Ph ◽  
Potassium Hydrogen ◽  
Optimization Of Fermentation

The paper concentrated on the optimization of the recombinant strain BL21 (DE3)-PE7-Nit. The component of culture medium and the culture conditions were optimized. The optimized medium was: yeast extract 10 g/l, L-glutamate sodium 8 g/l, MgSO4.7H2O 0.7 g/l, Isopropyl-β-D-thiogalactopyranoside 0.3 mmol/L, potassium hydrogen phosphate 0.5 g / L, phosphate Potassium 0.5 g / L and the culture condition was: initial pH 7.0, inoculum 2%. The result showed that the activity of nitrilase prepared with these conditions increased by 130.37 % through optimization.

scholarly journals Efficient Biofilm-Based Fermentation Strategies for L-Threonine Production by Escherichia coli

2019 ◽  
Vol 10 ◽  
Author(s):  
Tianpeng Chen ◽  
Na Liu ◽  
Peifang Ren ◽  
Xun Xi ◽  
Leyun Yang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Threonine Production

Factors Improvingl-Threonine Production by a Threel-Threonine Biosynthetic Genes-amplified Recombinant Strain ofBrevibacterium lactofermentum

1994 ◽  
Vol 58 (4) ◽  
pp. 768-770 ◽  
Author(s):  
Masaaki Ishida ◽  
Hiroki Kawashima ◽  
Katsuaki Sato ◽  
Ken-ichi Hashiguchi ◽  
Hisao Ito ◽  
...  
Keyword(s):  
Recombinant Strain ◽  
Biosynthetic Genes ◽  
Threonine Production

scholarly journals Metabolic engineering of a reduced-genome strain of Escherichia coli for L-threonine production

2009 ◽  
Vol 8 (1) ◽  
pp. 2 ◽  
Author(s):  
Jun Lee ◽  
Bong Sung ◽  
Mi Kim ◽  
Frederick R Blattner ◽  
Byoung Yoon ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Reduced Genome ◽  
Threonine Production

Improved method for expression and isolation of the Mycoplasma hominis arginine deiminase from the recombinant strain of Escherichia coli

2013 ◽  
Vol 167 (4) ◽  
pp. 420-426 ◽  
Author(s):  
Lyubov R. Fayura ◽  
Yuriy R. Boretsky ◽  
Yuriy V. Pynyaha ◽  
Denys N. Wheatley ◽  
Andriy A. Sibirny
Keyword(s):  
Escherichia Coli ◽  
Recombinant Strain ◽  
Mycoplasma Hominis ◽  
Arginine Deiminase ◽  
Improved Method

Development of sucrose-utilizing Escherichia coli K-12 strain by cloning β-fructofuranosidases and its application for l-threonine production

2010 ◽  
Vol 88 (4) ◽  
pp. 905-913 ◽  
Author(s):  
Jeong Wook Lee ◽  
Sol Choi ◽  
Jin Hwan Park ◽  
Claudia E. Vickers ◽  
Lars K. Nielsen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Threonine Production ◽  
K 12
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