Study on Optimization of Fermentation Condition for Nitrilase-Producer Escherichia Coli BL21 (DE3)/pET-Nit
2011 ◽
Vol 175-176
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pp. 192-196
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Keyword(s):
The paper concentrated on the optimization of the recombinant strain BL21 (DE3)-PE7-Nit. The component of culture medium and the culture conditions were optimized. The optimized medium was: yeast extract 10 g/l, L-glutamate sodium 8 g/l, MgSO4.7H2O 0.7 g/l, Isopropyl-β-D-thiogalactopyranoside 0.3 mmol/L, potassium hydrogen phosphate 0.5 g / L, phosphate Potassium 0.5 g / L and the culture condition was: initial pH 7.0, inoculum 2%. The result showed that the activity of nitrilase prepared with these conditions increased by 130.37 % through optimization.
2013 ◽
Vol 850-851
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pp. 1172-1175
2011 ◽
Vol 71-78
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pp. 2294-2297
Keyword(s):
2012 ◽
Vol 430-432
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pp. 395-398
Keyword(s):
2011 ◽
Vol 230-232
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pp. 1119-1122
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2013 ◽
Vol 781-784
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pp. 836-839
2014 ◽
Vol 1010-1012
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pp. 69-72