scholarly journals Creation and characterization of an airway epithelial cell line for stable expression of CFTR variants

2016 ◽  
Vol 15 (3) ◽  
pp. 285-294 ◽  
Author(s):  
Laura B. Gottschalk ◽  
Briana Vecchio-Pagan ◽  
Neeraj Sharma ◽  
Sangwoo T. Han ◽  
Arianna Franca ◽  
...  
2001 ◽  
Vol 280 (1) ◽  
pp. L127-L133 ◽  
Author(s):  
S. Tavakoli ◽  
M. J. Cowan ◽  
T. Benfield ◽  
C. Logun ◽  
J. H. Shelhamer

Human airway epithelial cell release of interleukin (IL)-6 in response to lipid mediators was studied in an airway cell line (BEAS-2B). Prostaglandin (PG) E2(10−7M) treatment caused an increase in IL-6 release at 2, 4, 8, and 24 h. IL-6 release into the culture medium at 24 h was 3,396 ± 306 vs. 1,051 ± 154 pg/ml (PGE2-treated cells vs. control cells). PGE2(10−7to 10−10M) induced a dose-related increase in IL-6 release at 24 h. PGF2α(10−6M) treatment caused a similar effect to that of PGE2(10−7M). PGE2analogs with relative selectivity for PGE2receptor subtypes were studied. Sulprostone, a selective agonist for the EP-3 receptor subtype had no effect on IL-6 release. 11-Deoxy-16,16-dimethyl-PGE2, an EP-2/4 agonist, and 17-phenyl trinor PGE2, an agonist selective for the EP-1 > EP-3 receptor subtype (10−6to 10−8M), caused dose-dependent increases in IL-6 release. 8-Bromo-cAMP treatment resulted in dose-related increases in IL-6 release. RT-PCR of BEAS-2B cell mRNA demonstrated mRNA for EP-1, EP-2, and EP-4 receptors. After PGE2treatment, increases in IL-6 mRNA were noted at 4 and 18 h. Therefore, PGE2increases airway epithelial cell IL-6 production and release.


Author(s):  
Jennifer Davies ◽  
Rachel Corkhill ◽  
Steve Christmas ◽  
Paul McNamara ◽  
Brian Flanagan

2009 ◽  
Vol 149 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Takahito Chiba ◽  
Shigeharu Ueki ◽  
Wataru Ito ◽  
Hikari Kato ◽  
Masahide Takeda ◽  
...  

1998 ◽  
Vol 30 (7) ◽  
pp. 3366-3367 ◽  
Author(s):  
J Nakajima ◽  
M Ono ◽  
J Kobayashi ◽  
M Takeda ◽  
M Kawauchi ◽  
...  

2020 ◽  
Vol 19 ◽  
pp. S113-S114
Author(s):  
G. Livnat-Levanon ◽  
J. Moncivaiz ◽  
L. Strecker ◽  
A. Ostmann ◽  
J. Clancy ◽  
...  

1994 ◽  
Vol 112 (1) ◽  
pp. 169-175 ◽  
Author(s):  
Anthony C. Chao ◽  
Jonathan B. Zifferblatt ◽  
John A. Wagner ◽  
Y.-J. Dong ◽  
Dieter C. Gruenert ◽  
...  

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