Simultaneous determination of 3,3′,4′,5,7-pentamethylquercetin and its possible metabolite 3,3′,4′,7-tetramethylquercetin in dog plasma by liquid chromatography–tandem mass spectrometry and its application to preclinical pharmacokinetic study

2011 ◽  
Vol 879 (23) ◽  
pp. 2339-2344 ◽  
Author(s):  
Xiaomei Chen ◽  
Dongyan Li ◽  
Yan Hu ◽  
Manwen Jin ◽  
Liping Zhou ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Simultaneous Determination ◽  
Pharmacokinetic Study ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Dog Plasma ◽  
Liquid Chromatography Tandem Mass

scholarly journals Liquid Chromatography-Tandem Mass Spectrometry Simultaneous Determination and Pharmacokinetic Study of Fourteen Alkaloid Components in Dog Plasma after Oral Administration of Corydalis bungeana Turcz Extract

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1927 ◽  
Author(s):  
Hongrui Dong ◽  
Guanyun Yan ◽  
Zhibin Wang ◽  
Chengcui Wu ◽  
Binbin Cui ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Oral Administration ◽  
Simultaneous Determination ◽  
Pharmacokinetic Study ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Dog Plasma ◽  
Liquid Chromatography Tandem Mass

A rapid and sensitive Ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous determination of fourteen alkaloids in beagle dog plasma after a single oral dose of the Corydalis bungeana Turcz (C. bungeana) extract selected bifendate as the internal standard (IS). The plasma samples were preprocessed by liquid-liquid extraction (LLE) with aether before separation on an Agilent SB-C18 column (1.8 µm, 150 × 2.1 mm) using a gradient elution program. The mobile phase consists of 0.2% acetic acid and acetonitrile at the flow rate of 0.3 mL/min. In the positive ion mode, the analytes were detected by multiple reaction monitoring (MRM). The results indicated that calibration curves for fourteen analytes have good linearity (R2 = 0.9904). The lower limits of quantification (LLOQ) of fourteen alkaloids and IS were all over 4.87 ng/mL and the matrix effects ranged from 94.08% to 102.76%. The mean extraction recoveries of Quality control samples at low (LQC), medium (MQC) and high (HQC) and IS were all more than 78.03%. The intra- and inter-day precision (R.S.D.%) also met the criterion, at the same time the deviation of assay accuracies (R.E) ranged from −13.70% to 14.40%. The Tmax values of fourteen alkaloids were no more than 1 h. The range of Cmax was from 74.16 ± 8.71 to 2256 ± 255.9 ng/mL. The assay was validated in the light of the regulatory bioanalytical guidelines and proved acceptable, which was successfully applied to a pharmacokinetic study of these compounds in beagle dogs after oral administration of Corydalis bungeana Turcz extract.

Sign in / Sign up

Export Citation Format

Share Document