Determination of Ochratoxin-A in the brain microdialysates and plasma of awake, freely moving rats using ultra high performance liquid chromatography fluorescence detection method

2019 ◽  
Vol 1125 ◽  
pp. 121700 ◽  
Author(s):  
Elif Mine Öncü Kaya ◽  
Orhan Tansel Korkmaz ◽  
Duygu Yeniceli Uğur ◽  
Erol Şener ◽  
A. Neşe Tunçel ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ochratoxin A ◽  
Fluorescence Detection ◽  
High Performance ◽  
Detection Method ◽  
Freely Moving Rats ◽  
Freely Moving ◽  
The Brain

A Rapid High-Performance Liquid Chromatography with Fluorescence Detection Method Developed To Analyze Ochratoxin A in Wine

2008 ◽  
Vol 71 (10) ◽  
pp. 2133-2137 ◽  
Author(s):  
ALESSIO TAFURI ◽  
GIUSEPPE MECA ◽  
ALBERTO RITIENI
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ochratoxin A ◽  
Fluorescence Detection ◽  
High Performance ◽  
Detection Method ◽  
Direct Injection ◽  
Mean Value ◽  
Inexpensive Method ◽  
Relative Standard

A rapid, sensitive, reproducible, and inexpensive method of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) for the analysis of ochratoxin A (OTA) in wine was developed. It is characterized by direct injection of the wine into the HPLC apparatus, with no need of extraction or cleanup. The method uses acetonitrile, water, and acetic acid (49:49:2, vol/vol/vol, respectively) as the isocratic mobile phase and a 5-mu;m monolithic C18 column (100 by 3 mm inside diameter). The relative standard deviation obtained in the OTA determination varied between 0.22 and 1.76%, with a mean value of 0.89%, in samples with concentrations between 0.10 and 100 ng/ml. The recovery of OTA ranged from 102% in samples spiked with 1 ng/ml OTA to 120% in samples with 0.10 ng/ml OTA. The method compared favorably with a published method based on an immunoaffinity column cleanup and a chromatographic assay with a C18 conventional HPLC column.

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