The Effect of Helicobacter felis and Helicobacter bizzozeronii on the Gastric Mucosa in Mongolian Gerbils: a Sequential Pathological Study

2006 ◽  
Vol 135 (4) ◽  
pp. 226-236 ◽  
Author(s):  
M. De Bock ◽  
K. D’Herde ◽  
L. Duchateau ◽  
A. Hellemans ◽  
A. Decostere ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Mongolian Gerbils ◽  
Pathological Study ◽  
Helicobacter Felis

scholarly journals Role of Helicobacter felis in Chronic Canine Gastritis

1992 ◽  
Vol 29 (6) ◽  
pp. 487-494 ◽  
Author(s):  
A. Lee ◽  
S. Krakowka ◽  
J. G. Fox ◽  
G. Otto ◽  
K. A. Eaton ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Plasma Cells ◽  
Serum Antibody ◽  
Electron Microscopic Examination ◽  
Gastric Mucus ◽  
Electron Microscopic ◽  
Helicobacter Felis ◽  
Large Numbers ◽  
Histopathologic Changes ◽  
Gastric Parietal Cells

Five gnotobiotic Beagle dogs were orally inoculated with a pure culture of Helicobacter felis. The remaining two littermates served as contact controls. Thirty days after infection, all animals were euthanatized and specimens were collected for evaluation. In infected dogs, H. felis was recovered from all areas of the stomach. Colonization was heaviest in the fundus and antrum. H. felis was not cultured from any segment of the gastrointestinal tract distal to the duodenum. Two weeks after infection, all five infected dogs had detectable IgM and IgG serum antibody to H. felis, whereas control dogs had no measurable H. felis serum antibody throughout the study. Histopathologic changes in the stomachs of infected dogs included large numbers of lymphoid nodules throughout all regions of the gastric mucosa and were most numerous in the fundus and body. A mild, diffuse lymphocytic infiltrate with small numbers of plasma cells and eosinophils was also present in the subglandular region of all portions of the gastric mucosa. Electron microscopic examination revealed large numbers of spiral-shaped H. felis in gastric mucus adjacent to or superimposed over the areas of inflammation. Occasionally, however, H. felis was observed within the canaliculi of gastric parietal cells. Histopathologic changes in the stomachs of the contact control dogs were limited to focal infiltrates of eosinophils and small aggregates of lymphocytes in the subglandular portions of the gastric mucosa in one animal. Infection with H. felis is a likely cause of naturally occurring lymphofollicular gastritis.

scholarly journals Helicobacter heilmannii sp. nov., isolated from feline gastric mucosa

2012 ◽  
Vol 62 (2) ◽  
pp. 299-306 ◽  
Author(s):  
A. Smet ◽  
B. Flahou ◽  
K. D’Herde ◽  
P. Vandamme ◽  
I. Cleenwerck ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Sequence Similarity ◽  
Protein Profiling ◽  
Rrna Gene ◽  
New Taxon ◽  
Helicobacter Felis ◽  
Helicobacter Heilmannii ◽  
Gene Sequence Analysis ◽  
Taxonomic Approach ◽  
Microaerobic Conditions

Three Gram-negative, microaerophilic bacteria, strains ASB1T, ASB2 and ASB3, with a corkscrew-like morphology isolated from the gastric mucosa of cats were studied using a polyphasic taxonomic approach. The isolates grew on biphasic culture plates under microaerobic conditions at 37 °C and exhibited urease, oxidase and catalase activities. They were also able to grow in colonies on dry agar plates. Based on 16S rRNA gene sequence analysis, ASB1T, ASB2 and ASB3 were identified as members of the genus Helicobacter and showed 98 to 99 % sequence similarity to strains of Helicobacter felis, Helicobacter bizzozeronii, ‘Candidatus Helicobacter heilmannii’, Helicobacter cynogastricus, Helicobacter baculiformis and Helicobacter salomonis, six related Helicobacter species previously detected in feline or canine gastric mucosa. Sequencing of the partial hsp60 gene demonstrated that ASB1T, ASB2 and ASB3 constitute a separate taxon among the feline and canine Helicobacter species. The urease gene sequences of ASB1T, ASB2 and ASB3 showed approximately 91 % similarity to those of ‘Candidatus Helicobacter heilmannii’. Protein profiling, the absence of alkaline phosphatase activity and several other biochemical characteristics also allowed strains ASB1T, ASB2 and ASB3 to be differentiated from other Helicobacter species of feline or canine gastric origin. The results of this polyphasic taxonomic study show that the cultured isolates constitute a new taxon corresponding to ‘Candidatus Helicobacter heilmannii’, which was previously demonstrated in the stomach of humans, wild felidae, cats and dogs. The name Helicobacter heilmannii sp. nov. is proposed for these isolates; the type strain is ASB1T ( = DSM 23983T = LMG 26292T).

scholarly journals Attenuated apoptosis in H.pylori-colonized gastric mucosa of mongolian gerbils

2000 ◽  
Vol 118 (4) ◽  
pp. A746
Author(s):  
Hidekazu Suzuki ◽  
Shoichi Nagahashi ◽  
Koichi Seto ◽  
Akemi Kai ◽  
Makoto Suematsu ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Mongolian Gerbils

Helicobacter felis and Helicobacter bizzozeronii induce gastric parietal cell loss in Mongolian gerbils

2006 ◽  
Vol 8 (2) ◽  
pp. 503-510 ◽  
Author(s):  
Manuelle De Bock ◽  
Annemie Decostere ◽  
Ann Hellemans ◽  
Freddy Haesebrouck ◽  
Richard Ducatelle
Keyword(s):  
Parietal Cell ◽  
Cell Loss ◽  
Mongolian Gerbils ◽  
Gastric Parietal Cell ◽  
Helicobacter Felis

scholarly journals Helicobacter cynogastricus sp. nov., isolated from the canine gastric mucosa

2006 ◽  
Vol 56 (7) ◽  
pp. 1559-1564 ◽  
Author(s):  
K. Van den Bulck ◽  
A. Decostere ◽  
M. Baele ◽  
P. Vandamme ◽  
J. Mast ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Gene Sequence ◽  
Novel Species ◽  
Protein Profiling ◽  
Rrna Gene ◽  
The Novel ◽  
Helicobacter Felis ◽  
Urease Gene ◽  
Gene Sequence Analysis ◽  
Sds Page

A Gram-negative, microaerophilic helical rod, isolated from the gastric mucosa of a dog and designated strain JKM4T, was subjected to a polyphasic taxonomic study. The tightly coiled organism, measuring 10–18 μm long and up to 1 μm wide, was motile by means of multiple sheathed flagella located at both ends of the cell and by a periplasmic fibril running along the external side of the helix. Strain JKM4T grew preferably on biphasic culture plates or on very moist agar. Coccoid forms predominated in cultures older than 4 days as well as in growth obtained on dry agar plates. The strain grew at 30 and 37 °C, but not at 25 or 42 °C and exhibited urease, oxidase and catalase activities. On the basis of 16S rRNA gene sequence analysis, the novel isolate was identified as a member of the genus Helicobacter and showed > 97 % similarity to Helicobacter felis, Helicobacter bizzozeronii and Helicobacter salomonis, three species previously isolated from the canine gastric mucosa. Protein profiling of strain JKM4T using SDS-PAGE revealed a pattern different from those of other Helicobacter species of mammalian gastric origin and from Helicobacter canis. Additionally, the urease gene sequence of strain JKM4T was different from those of urease genes of H. felis, H. bizzozeronii, H. salomonis and ‘Candidatus Helicobacter heilmannii’. It is thus proposed that strain JKM4T (=LMG 23188T) represents a novel species within this genus, Helicobacter cynogastricus sp. nov.

Analysis of the microbial ecology between Helicobacter pylori and the gastric microbiota of Mongolian gerbils

2014 ◽  
Vol 63 (1) ◽  
pp. 129-137 ◽  
Author(s):  
Cynthia Zaman ◽  
Takako Osaki ◽  
Tomoko Hanawa ◽  
Hideo Yonezawa ◽  
Satoshi Kurata ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Microbial Ecology ◽  
Inhibitory Effect ◽  
Mongolian Gerbils ◽  
Group 2 ◽  
H Pylori ◽  
Gastric Microbiota ◽  
Group 1

Animal models are essential for in vivo analysis of Helicobacter-related diseases. Mongolian gerbils are used frequently to study Helicobacter pylori-induced gastritis and its consequences. The presence of some gastric microbiota with a suppressive effect on H. pylori suggests inhibitory gastric bacteria against H. pylori infection. The aim of the present study was to analyse the microbial ecology between H. pylori and the gastric microbiota of Mongolian gerbils. Gastric mucosa samples of H. pylori-negative and -positive gerbils were orally inoculated to five (Group 1) and six (Group 2) gerbils, respectively, and the gerbils were challenged with H. pylori infection. The colonization rate (40 %) of H. pylori in Group 1 gerbils was lower than the rate (67 %) in Group 2 gerbils. Culture filtrate of the gastric mucosa samples of Group 1 gerbils inhibited the in vitro growth of H. pylori. Three lactobacilli species, Lactobacillus reuteri, Lactobacillus johnsonii and Lactobacillus murinus, were isolated by anaerobic culture from the gerbils in Groups 1 and 2, and identified by genomic sequencing. It was demonstrated that the three different strains of lactobacilli exhibited an inhibitory effect on the in vitro growth of H. pylori. The results suggested that lactobacilli are the dominant gastric microbiota of Mongolian gerbils and the three lactobacilli isolated from the gastric mucosa samples with an inhibitory effect on H. pylori might have an anti-infective effect against H. pylori.

scholarly journals Healthy Cats Are Commonly Colonized with “Helicobacter heilmannii” That Is Associated with Minimal Gastritis

1999 ◽  
Vol 37 (1) ◽  
pp. 189-194 ◽  
Author(s):  
C. R. Norris ◽  
S. L. Marks ◽  
K. A. Eaton ◽  
S. Z. Torabian ◽  
R. J. Munn ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
16S Rrna ◽  
Light Microscopy ◽  
Gastric Biopsy ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Helicobacter Felis ◽  
Helicobacter Heilmannii ◽  
Biopsy Specimens ◽  
The One

Gastric Helicobacter infection in healthy pet cats is not well characterized. We performed endoscopy with gastric biopsy on 15 healthy pet cats that were rigorously screened to exclude underlying or concurrent diseases that might affect Helicobactercolonization. Gastric mucosa biopsy specimens were examined by histology, culture, and PCR for the presence ofHelicobacter infection and by histology for the presence of gastritis. Of 15 cats, all but 1 had gastricHelicobacter-like organisms (GHLOs) on examination by light microscopy, and in the one histologically negative cat, GHLOs were detected by PCR. Gastric inflammation was mild or was absent for all cats. No Helicobacter species were identified by culture. Analysis of the 16S rRNA sequence from Helicobacter strains from 10 cats showed that all bacteria were closely related toHelicobacter felis, although there was heterogeneity among the sequences. These results suggest that the gastric mucosa of healthy pet cats is commonly colonized with an uncultivatedHelicobacter that is closely related to H. felis, is associated with little or no gastritis, and shows heterogeneity in its 16S rRNA sequence. The epithet “Helicobacter heilmannii” continues to be an appropriate working designation for these bacteria.

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