Seroprevalence of Anaplasma phagocytophilum, Borrelia burgdorferi, baBesia gibsoni, Ehrlichia canis and Ehrlichia chaffeensis in dogs from the republic of Korea

2019 ◽  
Vol 166 ◽  
pp. 132
Author(s):  
S. Lee ◽  
H. Lee ◽  
J. Park ◽  
S. Yoon ◽  
B. So ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Ehrlichia Canis ◽  
Republic Of Korea ◽  
Ehrlichia Chaffeensis ◽  
Babesia Gibsoni ◽  
The Republic

scholarly journals Geographical Distribution of Borrelia burgdorferi sensu lato in Ticks Collected from Wild Rodents in the Republic of Korea

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 866
Author(s):  
Seong Yoon Kim ◽  
Tae-Kyu Kim ◽  
Tae Yun Kim ◽  
Hee Il Lee
Keyword(s):  
Sequence Analysis ◽  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Jeju Island ◽  
Republic Of Korea ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Wild Rodents ◽  
The Republic ◽  
Tick Vectors ◽  
Pcr Targeting

Lyme disease is a tick-borne zoonotic disease caused by Borrelia burgdorferi sensu lato (s. l.) via transmission cycles involving competent tick vectors and vertebrate reservoirs. Here, we determined the prevalence and distribution of Borrelia genospecies in 738 ticks of at least three species from wild rodents in nine regions of the Republic of Korea (ROK). Ticks were analyzed using nested PCR targeting partial flagellin B gene sequences, followed by sequence analysis. The prevalence of Borrelia infection was 33.6%, and the most common genospecies were B. afzelii (62.5%), B. valaisiana (31.9%), B. yangtzensis (2.4%), B. garinii (1.6%), and B. tanukii (1.6%). Borrelia afzelii was found in all regions except Jeju Island; this predominant genospecies was found in the northern and central sampling regions. Borrelia valaisiana, B. yangtzensis, and B. tanukii were found only in the southern regions with B. valaisiana being the most common, whereas B. yangtzensis and B. tanukii were only found on Jeju Island. Our study is the first to describe the nationwide prevalence of B. burgdorferi s. l. in ticks from wild rodents in the ROK. Continuous surveillance in ticks, animals, humans, and different regions is required to avoid disease distribution and possible transmission to humans in the ROK.

scholarly journals Molecular Detection and Phylogeny of Tick-Borne Pathogens in Ticks Collected from Dogs in the Republic of Korea

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 613
Author(s):  
A-Tai Truong ◽  
Jinhyeong Noh ◽  
Yeojin Park ◽  
Hyun-Ji Seo ◽  
Keun-Ho Kim ◽  
...  
Keyword(s):  
Disease Surveillance ◽  
Large Scale ◽  
Phylogenetic Analyses ◽  
Republic Of Korea ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Haemaphysalis Longicornis ◽  
Babesia Gibsoni ◽  
The Republic ◽  
And Control

Ticks are important vectors of various pathogens that result in clinical illnesses in humans and domestic and wild animals. Information regarding tick infestations and pathogens transmitted by ticks is important for the identification and prevention of disease. This study was a large-scale investigation of ticks collected from dogs and their associated environments in the Republic of Korea (ROK). It included detecting six prevalent tick-borne pathogens (Anaplasma spp., A. platys, Borrelia spp., Babesia gibsoni, Ehrlichia canis, and E. chaffeensis). A total of 2293 ticks (1110 pools) were collected. Haemaphysalis longicornis (98.60%) was the most frequently collected tick species, followed by Ixodes nipponensis (0.96%) and H. flava (0.44%). Anaplasma spp. (24/1110 tick pools; 2.16%) and Borrelia spp. (4/1110 tick pools; 0.36%) were detected. The phylogenetic analyses using 16S rRNA genes revealed that the Anaplasma spp. detected in this study were closely associated with A. phagocytophilum reported in humans and rodents in the ROK. Borrelia spp. showed phylogenetic relationships with B. theileri and B. miyamotoi in ticks and humans in Mali and Russia. These results demonstrate the importance of tick-borne disease surveillance and control in dogs in the ROK.

scholarly journals Molecular investigation of tick-borne pathogens in ticks removed from tick-bitten humans in the southwestern region of the Republic of Korea

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252992
Author(s):  
Mi Seon Bang ◽  
Choon-Mee Kim ◽  
Sang-Hyun Pyun ◽  
Dong-Min Kim ◽  
Na Ra Yun
Keyword(s):  
Tick Species ◽  
Spotted Fever ◽  
Common Species ◽  
Republic Of Korea ◽  
Spotted Fever Group ◽  
Haemaphysalis Longicornis ◽  
Babesia Gibsoni ◽  
Specific Pcr ◽  
Rickettsia Monacensis ◽  
The Republic

In this study, we investigated the presence of tick-borne pathogens in ticks removed from tick-bitten humans in the southwestern provinces of the Republic of Korea (ROK). We identified 33 ticks from three tick species, namely Amblyomma testudinarium (60.6%), Haemaphysalis longicornis (27.3%), and Ixodes nipponensis (12.1%) in order of occurrence via morphology and 16S rDNA-targeting polymerase chain reaction (PCR). Tick-borne pathogens were detected in 16 ticks using pathogen-specific PCR. From the results, 12 ticks (36.4%) tested positive for spotted fever group (SFG) Rickettsia: Rickettsia monacensis (1/12), R. tamurae (8/12), and Candidatus Rickettsia jingxinensis (3/12). Three ticks (9.1%) were positive for Anaplasma phagocytophilum. In addition, three ticks (9.1%) tested positive for Babesia gibsoni (1/3) and B. microti (2/3). In conclusion, we identified three tick species; the most common species was A. testudinarium, followed by H. longicornis and I. nipponensis. SFG Rickettsia, A. phagocytophilum, and Babesia spp. were the most frequently detected pathogens in ticks removed from tick-bitten humans. To our knowledge, this is the first report of R. tamurae and Ca. R. jingxinensis detection in Korea. The present results will contribute to the understanding of tick-borne infections in animals and humans in the ROK.

scholarly journals Evaluation of Antibiotic Susceptibilities of Ehrlichia canis, Ehrlichia chaffeensis, and Anaplasma phagocytophilum by Real-Time PCR

2004 ◽  
Vol 48 (12) ◽  
pp. 4822-4828 ◽  
Author(s):  
S. Branger ◽  
J. M. Rolain ◽  
D. Raoult
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Anaplasma Phagocytophilum ◽  
Point Mutations ◽  
Reference Method ◽  
Ehrlichia Canis ◽  
New Drugs ◽  
Ehrlichia Chaffeensis ◽  
Real Time Quantitative Pcr ◽  
Highly Active

ABSTRACT We determined MICs of antibiotics against Anaplasma phagocytophilum, Ehrlichia chaffeensis, and Ehrlichia canis by real-time quantitative PCR. The doubling times of the organisms were established: 19 h for E. chaffeensis, 26 h for A. phagocytophilum, and 28 h for E. canis. In comparison to the reference method for determining sensitivities, which uses Diff-Quick staining, our PCR assay was very sensitive and specific. We confirmed that doxycycline and rifampin are highly active against these bacteria and found variable susceptibilities to fluoroquinolones; A. phagocytophilum was susceptible, but E. canis and E. chaffeensis were only partly susceptible. β-Lactam compounds, cotrimoxazole, macrolide compounds, and telithromycin showed no activity against any of the three organisms. Thiamphenicol was found to be more active than chloramphenicol. For the first time, we showed that these three species have numerous point mutations in their 23S RNA genes, with those at positions 754, 2057, 2058, 2059, and 2611 (Escherichia coli numbering) known to confer resistance to macrolide compounds in other bacteria. The role of each of these mutations in resistance to these drugs should be investigated in the future. Our study confirms previous reports that quantitative PCR is a reliable method for determining antibiotic susceptibility; therefore, it might be useful for screening new drugs.

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