Quantification of Epstein-Barr virus load in Argentinean transplant recipients using real-time PCR

2004 ◽  
Vol 31 (1) ◽  
pp. 58-65 ◽  
Author(s):  
Judith Yancoski ◽  
Silvia Danielian ◽  
Juan Ibañez ◽  
Amalia Turconi ◽  
Miriam Cuarterolo ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Epstein Barr Virus ◽  
Transplant Recipients ◽  
Virus Load ◽  
Barr Virus ◽  
Epstein Barr

Quantitative Analysis of Epstein-Barr Virus Load by Using a Real-Time PCR Assay

1999 ◽  
Vol 37 (1) ◽  
pp. 132-136 ◽  
Author(s):  
Hiroshi Kimura ◽  
Makoto Morita ◽  
Yumi Yabuta ◽  
Kiyotaka Kuzushima ◽  
Koji Kato ◽  
...  
Keyword(s):  
Real Time ◽  
Infectious Mononucleosis ◽  
Real Time Pcr ◽  
Copy Number ◽  
Epstein Barr Virus ◽  
Pcr Assay ◽  
Virus Load ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

To measure the virus load in patients with symptomatic Epstein-Barr virus (EBV) infections, we used a real-time PCR assay to quantify the amount of EBV DNA in blood. The real-time PCR assay could detect from 2 to over 107 copies of EBV DNA with a wide linear range. We estimated the virus load in peripheral blood mononuclear cells (PBMNC) from patients with symptomatic EBV infections. The mean EBV-DNA copy number in the PBMNC was 103.7 copies/μg of DNA in patients with EBV-related lymphoproliferative disorders, 104.1 copies/μg of DNA in patients with chronic active EBV infections, and 102.2copies/μg of DNA in patients with infectious mononucleosis. These numbers were significantly larger than those in either posttransplant patients or immunocompetent control patients without EBV-related diseases. In a patient with infectious mononucleosis, the virus load decreased as the symptoms resolved. The copy number of EBV DNA in PBMNC from symptomatic EBV infections was correlated with the EBV-positive cell number determined by the in situ hybridization assay (r = 0.842; P < 0.0001). These results indicate that the real-time PCR assay is useful for diagnosing symptomatic EBV infection and for monitoring the virus load.

Quantification of Epstein-Barr virus load in peripheral blood of human immunodeficiency virus-infected patients using real-time PCR

2001 ◽  
Vol 65 (3) ◽  
pp. 543-552 ◽  
Author(s):  
Axelle Dehee ◽  
Catherine Asselot ◽  
Tristan Piolot ◽  
Christine Jacomet ◽  
Willy Rozenbaum ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Peripheral Blood ◽  
Epstein Barr Virus ◽  
Virus Load ◽  
Barr Virus ◽  
Immunodeficiency Virus ◽  
Epstein Barr

scholarly journals Comparison of Commercial Real-Time PCR Assays for Quantification of Epstein-Barr Virus DNA

2005 ◽  
Vol 43 (5) ◽  
pp. 2053-2057 ◽  
Author(s):  
G. Ruiz ◽  
P. Pena ◽  
F. de Ory ◽  
J. E. Echevarria
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Pcr Assays ◽  
Epstein Barr
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