Comparison of meteorology created by profilometry and digital microscopy on polished dentine in an erosion/abrasion model

The process of moving to a fully digital microscopy laboratory requires changes in instrumentation, computing hardware, computing software, data storage systems, and data networks, as well as in the operating procedures of each facility. Moving from analog to digital systems in the microscopy laboratory is similar to the instrumentation projects being undertaken in many scientific labs. A central problem of any of these projects is to create the best combination of hardware and software to effectively control the parameters of data collection and then to actually acquire data from the instrument. This problem is particularly acute for the microscopist who wishes to "digitize" the operation of a transmission or scanning electron microscope. Although the basic physics of each type of instrument and the type of data (images & spectra) generated by each are very similar, each manufacturer approaches automation differently. The communications interfaces vary as well as the command language used to control the instrument.

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Use of digital microscopy in process control during the manufacture of rubber-modified thermoplastics

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100165549 ◽

1996 ◽

Vol 54 ◽

pp. 616-617

Author(s):

M. T. Dineen

Keyword(s):

Ccd Camera ◽

Operating Costs ◽

Hard Copy ◽

Production Plant ◽

Digital Microscopy ◽

Nih Image ◽

Transmission Electron ◽

Conventional Film ◽

Product Loss ◽

Image Collection

The production of rubber modified thermoplastics can exceed rates of 30,000 pounds per hour. If a production plant needs to equilibrate or has an upset, that means operating costs and lost revenue. Results of transmission electron microscopy (TEM) can be used for process adjustments to minimize product loss. Conventional TEM, however, is not a rapid turnaround technique. The TEM process was examined, and it was determined that 50% of the time it took to complete a polymer sample was related to film processing, even when using automated equipment. By replacing the conventional film portion of the process with a commercially available system to digitally acquire the TEM image, a production plant can have the same TEM image in the control room within 1.5 hours of sampling.A Hitachi H-600 TEM Operated at 100 kV with a tungsten filament was retrofitted with a SEMICAPS™ image collection and processing workstation and a KODAK MEGAPLUS™ charged coupled device (CCD) camera (Fig. 1). Media Cybernetics Image-Pro Plus software was included, and connections to a Phaser II SDX printer and the network were made. Network printers and other PC and Mac software (e.g. NIH Image) were available. By using digital acquisition and processing, the time it takes to produce a hard copy of a digital image is greatly reduced compared to the time it takes to process film.

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Classification of endonasal HHT lesions using digital microscopy

Orphanet Journal of Rare Diseases ◽

10.1186/s13023-021-01801-9 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

F. Haubner ◽

A. Schneider ◽

H. Schinke ◽

M. Bertlich ◽

B. G. Weiss ◽

...

Keyword(s):

Quality Of Life ◽

Treatment Modalities ◽

Hereditary Haemorrhagic Telangiectasia ◽

Digital Microscopy ◽

Digital Microscope ◽

Lower Severity ◽

Gender Specific Differences ◽

Vegf Level

Abstract Background Recurrent spontaneous epistaxis is the most common clinical manifestation and the most debilitating symptom in hereditary haemorrhagic telangiectasia (HHT) patients. To this date, there exist only a classification of HHT patients by different genetic mutations. There is no standard classification for the mucocutaneous endonasal manifestations of HHT. The aim of the present study was to document the variety of endonasal HHT lesions using digital microscopy and to propose a clinical classification. Methods We recorded the endonasal HHT lesions of 28 patients using a digital microscope. We reconstructed the 3D images und videos recorded by digital microscope afterwards and classified the endonasal lesions of HHT in two classes: Grade A, presence of only flat telangiectasias in the mucosa level and Grade B, (additional) presence of raised berry or wart-like telangiectasia spots. We investigated also Haemoglobin level by routine laboratory procedures, plasma VEGF level by ELISA, Severity of epistaxis by epistaxis severity score (ESS) and quality of life by a linear visual analogue scale (VAS). Results We found a higher quality of life and a lower severity of epistaxis in Grade A patients in comparison to Grade B patients. No difference in plasma VEGF level and in Haemoglobin between Grad A patients and Grade B patients could be detected. Plasma VEGF levels showed no gender specific differences. It could also not be correlated to the extranasal manifestation. Conclusion The classification for endonasal manifestation of HHT proposed in this study indicates severity of epistaxis und quality of life. Digital microscopy with the ability of 3D reconstruction of images presents a useful tool for such classifications. The classification of endonasal HHT lesions using digital microscopy allows to evaluate the dynamic of HHT lesions in the course of time independent of examiner. This allows also to evaluate the efficacy of the different treatment modalities by dynamic of HHT lesions. Moreover digital microscopy is very beneficial in academic teaching of rare diseases.

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Sensitivity of quantitative symmetry measurement algorithms for convergent beam electron diffraction technique

Applied Microscopy ◽

10.1186/s42649-021-00060-z ◽

2021 ◽

Vol 51 (1) ◽

Author(s):

Hyeongsub So ◽

Ro Woon Lee ◽

Sung Taek Hong ◽

Kyou-Hyun Kim

Keyword(s):

Electron Diffraction ◽

Cross Correlation ◽

Structural Changes ◽

Small Strain ◽

Convergent Beam Electron Diffraction ◽

Digital Microscopy ◽

Beam Electron ◽

R Factor ◽

Electron Diffraction Technique ◽

Convergent Beam

AbstractWe investigate the sensitivity of symmetry quantification algorithms based on the profile R-factor (Rp) and the normalized cross-correlation (NCC) coefficient (γ). A DM (Digital Micrograph©) script embedded in the Gatan digital microscopy software is used to develop the symmetry quantification program. Using the Bloch method, a variety of CBED patterns are simulated and used to investigate the sensitivity of symmetry quantification algorithms. The quantification results show that two symmetry quantification coefficients are significantly sensitive to structural changes even for small strain values of < 1%.

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TEAM Malawi: Low Cost Digital Microscopy for Automated Lab Testing

Annals of Global Health ◽

10.1016/j.aogh.2017.03.008 ◽

2017 ◽

Vol 83 (1) ◽

pp. 4

Author(s):

D. De La Torre Campos ◽

J. Achempong ◽

A. Atta ◽

S. Claybon ◽

D. DeVincentis ◽

...

Keyword(s):

Low Cost ◽

Digital Microscopy

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Segmentation of digital microscopy data for the analysis of defect structures in materials using nonlinear diffusions

10.1117/12.777232 ◽

2008 ◽

Cited By ~ 1

Author(s):

Landis M. Huffman ◽

Jeff Simmons ◽

Ilya Pollak

Keyword(s):

Digital Microscopy ◽

Defect Structures ◽

Nonlinear Diffusions ◽

Microscopy Data

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PRE-MEETING SHORT COURSES: JULY 31

Microscopy and Microanalysis ◽

10.1017/s1431927605150302 ◽

2005 ◽

Vol 11 (I1) ◽

pp. 23-26

Keyword(s):

Freeze Substitution ◽

Specimen Preparation ◽

Digital Microscopy ◽

High Pressure Freezing ◽

Physical Sciences ◽

Special Events ◽

Nanoscale Imaging ◽

Art Exhibit ◽

Short Courses ◽

Full Day

Organizers: John Mansfield and Louis KerrAdditional fees required.SC01: Towards Nanoscale Imaging of Anything in VPSEM (including ESEM): From Basics to Current Practices. Full Day: 9:00 AM–5:00 PM, Room 317A.SC02: Image Processing and Analysis. Full Day: 9:00 AM–5:00 PM, Room 317B.SC03: Photoshop for Microscopy and Microanalysis. Full Day: 9:00 AM–5:00 PM, Room 318A.SC04: High Pressure Freezing Cryosectioning of Vitrified Samples for Tomography, and Freeze Substitution. Full Day: 9:00 AM–5:00 PM, Room 318B.SC05: Specimen Preparation for the Physical Sciences. Full Day: 9:00 AM–5:00 PM, Room 319A.SC06: Digital Microscopy and Image Analysis for Materials Characterization. Half Day: 9:00 AM–1:00 PM, Room 319B.SC07: Interpretation of Microstructure. Half-Day: 9:00 AM–1:00 PM, Room 323A.Special Events: Presidential happenings; IMS Henry Clifton Sorby award and lecture; and Art Exhibit.Educational Venues: Microscopic explorations: A workshop; and It's a Family Affair!

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