scholarly journals Elevated Snail expression in human gingival fibroblasts by cyclosporine A as the possible pathogenesis for gingival overgrowth

2015 ◽  
Vol 114 (12) ◽  
pp. 1181-1186 ◽  
Author(s):  
Yi-Hung Lin ◽  
Cheng-Chia Yu ◽  
Shiuan-Shinn Lee ◽  
Yu-Chao Chang
Keyword(s):  
Cyclosporine A ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Snail Expression ◽  
Gingival Overgrowth

Evaluation of inductive effects of different concentrations of cyclosporine A on MMP-1, MMP-2, MMP-3, TIMP-1, and TIMP-2 in fetal and adult human gingival fibroblasts

2019 ◽  
Vol 30 (3) ◽  
Author(s):  
Bahareh Nazemisalman ◽  
Neda Sajedinejad ◽  
Shayan Darvish ◽  
Surena Vahabi ◽  
Hoda Gudarzi
Keyword(s):  
Cyclosporine A ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Adult Group ◽  
Gelatinase Activity ◽  
Gingival Overgrowth ◽  
Adult Human ◽  
Single Donor ◽  
Inductive Effects

Abstract Background The etiology of gingival overgrowth due to cyclosporine A (CsA) is still unknown. The aim of this study was to determine the possible role of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) on extra-cellular matrix (ECM) homeostasis when treated with different levels of CsA and its difference between fetal and adult human gingival fibroblasts (HGFs). Methods Each group of cells (adult and fetal) was cultured in 40 wells that consisted of four different CsA treatment concentrations. Every 10 wells were treated with 0, 50, 100, and 150 ng/mL of CsA which makes a total of 80 wells. Supernatants of every well were used to determine the concentration of MMPs and TIMPs using the Elisa kits from Boster, CA, USA. Results MMP-1 level increased with the treatment of CsA when treated with 50 and 150 ng/mL of CsA (p = 0.02 and p = 0.04) as TIMP-1 decreased (p < 0.0001) in adult group; while in the fetal group, TIMP-1 level increased with treatment of 150 ng/mL (p < 0.0001). MMP-2 level increased in both adult and fetal groups (p < 0.0001). MMP-3 level decreased in adult group (p < 0.0001) but went up in fetal HGFs (p = 0.01) when treated with 150 ng/mL CsA. TIMP-2 level increased in all wells significantly when treated with CsA (p < 0.0001). The study showed that CsA affects secretion of MMPs and TIMPs. MMP-1 increment and TIMP-1 decrement were observed, which indicate more degradation of ECM. This may be due to single donor use in this study. TIMP-2 and MMP-2 were both more active when treated with CsA which may be due to the gelatinase activity of them and that in CsA gingival overgrowth. There was more inflammation rather than fibrosis.

scholarly journals Mechanism of Drug-induced Gingival Overgrowth: Phenytoin Inhibits the Apoptosis of Human Gingival Fibroblasts

2021 ◽  
Vol 20 (2) ◽  
pp. 109-116
Author(s):  
Reiri Takeuchi ◽  
Hiroko Matsumoto ◽  
Chieko Taguchi ◽  
Yuichiro Okada ◽  
Masaru Mizuta ◽  
...  
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Drug Induced ◽  
Gingival Overgrowth

scholarly journals Cyclosporin A and Phenytoin Modulate Inflammatory Responses

2009 ◽  
Vol 88 (12) ◽  
pp. 1131-1136 ◽  
Author(s):  
A.M.M. Suzuki ◽  
A. Yoshimura ◽  
Y. Ozaki ◽  
T. Kaneko ◽  
Y. Hara
Keyword(s):  
Cyclosporin A ◽  
Inflammatory Responses ◽  
Chinese Hamster ◽  
Human Gingival Fibroblasts ◽  
Common Side Effect ◽  
Gingival Fibroblasts ◽  
Toll Like Receptor ◽  
Gingival Overgrowth ◽  
Anti Epileptic Drug ◽  
Tlr4 Ligand

Gingival overgrowth is a common side-effect of administration of the immunosuppressant cyclosporin A and the anti-epileptic drug phenytoin. While cyclosporin-induced gingival overgrowth is often accompanied by gingival inflammation, phenytoin-induced gingival overgrowth usually forms fibrotic lesions. To determine whether these drugs alter the inflammatory responses of gingival fibroblasts, we investigated the effects of cyclosporin and phenytoin on Toll-like receptor (TLR)-mediated responses to microbial components. In Chinese hamster ovary reporter cell lines, cyclosporin alone triggered signaling, whereas phenytoin down-regulated signaling induced by the TLR2 or TLR4 ligand. In human gingival fibroblasts, cyclosporin alone did not induce evident inflammatory responses, but augmented the expression of CD54 and the production of interleukin (IL)-6 and IL-8 induced by TLR ligands, whereas phenytoin attenuated those responses. Cyclosporin also augmented CD54 expression in gingiva of mice injected with lipopolysaccharide. These results indicated that cyclosporin positively and phenytoin negatively modulated inflammatory responses of human gingival fibroblasts.

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