Scavenger receptor class B member 1 ( SCARB1 ) variants modulate hepatitis C virus replication cycle and viral load

2017 ◽  
Vol 67 (2) ◽  
pp. 237-245 ◽  
Author(s):  
Sandra Westhaus ◽  
Maximilian Deest ◽  
Anna T.X. Nguyen ◽  
Frauke Stanke ◽  
Dirk Heckl ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Viral Load ◽  
Hepatitis C ◽  
Virus Replication ◽  
Scavenger Receptor ◽  
Replication Cycle ◽  
Scavenger Receptor Class ◽  
Class B ◽  
Virus Replication Cycle

Scavenger Receptor Class B Type I Mediates Cell Entry of Hepatitis C Virus

2008 ◽  
Vol 36 (6) ◽  
pp. 1319-1325 ◽  
Author(s):  
ZS Jia ◽  
DW Du ◽  
YF Lei ◽  
X Wei ◽  
W Yin ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cho Cells ◽  
Hepg2 Cells ◽  
Scavenger Receptor ◽  
Chinese Hamster ◽  
Hcv Infection ◽  
Type I ◽  
Scavenger Receptor Class ◽  
Class B

This study assessed the functional role of human scavenger receptor class B type I (SR-BI) as a putative hepatitis C virus (HCV) receptor using Chinese hamster ovary (CHO) cells transfected with human SR-BI (CHO–huSR-BI). The expression of SR-BI by primary Tupaia hepatocytes (PTHs), human hepatocarcinoma cell line (HepG2) cells, untransfected CHO cells and CHO–huSR-BI cells was analysed by Western blotting. Receptor competition assays showed that anti-SR-BI antibodies that block the binding of soluble envelope glycoprotein E2 could prevent HCV infection. Pre-incubation of CHO–huSR-BI and HepG2 cells with anti-SR-BI antibodies resulted in marked inhibition of E2 binding. After incubation with HCV RNA-positive serum from a patient with chronic HCV infection, however, HCV infection could not be detected in CHO–huSR-BI cells, but was detected in PTHs. These results demonstrate that, whilst SR-BI represents an important cell surface molecule for HCV infection, the presence of SR-BI alone is insufficient for HCV entry.

scholarly journals Hepatitis C virus replication cycle

2010 ◽  
Vol 53 (3) ◽  
pp. 583-585 ◽  
Author(s):  
Ralf Bartenschlager ◽  
Francois-Loic Cosset ◽  
Volker Lohmann
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Replication ◽  
Replication Cycle ◽  
Virus Replication Cycle

56 Scavenger receptor class B type i mediates uptake and cross-presentation of hepatitis C virus by human dendritic cells

2006 ◽  
Vol 44 ◽  
pp. S26
Author(s):  
H. Barth ◽  
E.K. Schnober ◽  
C. Neumann-Haefelin ◽  
R. Thimme ◽  
H.E. Blum ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Scavenger Receptor ◽  
Type I ◽  
Cross Presentation ◽  
Scavenger Receptor Class ◽  
Class B ◽  
Human Dendritic Cells

scholarly journals Completion of Hepatitis C Virus Replication Cycle in Heterokaryons Excludes Dominant Restrictions in Human Non-liver and Mouse Liver Cell Lines

2011 ◽  
Vol 7 (4) ◽  
pp. e1002029 ◽  
Author(s):  
Anne Frentzen ◽  
Kathrin Hüging ◽  
Julia Bitzegeio ◽  
Martina Friesland ◽  
Sibylle Haid ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cell Lines ◽  
Virus Replication ◽  
Liver Cell ◽  
Mouse Liver ◽  
Replication Cycle ◽  
Mouse Liver Cell ◽  
Virus Replication Cycle

scholarly journals The postbinding activity of scavenger receptor class B type I mediates initiation of hepatitis C virus infection and viral dissemination

Hepatology ◽  
2012 ◽  
Vol 57 (2) ◽  
pp. 492-504 ◽  
Author(s):  
Muhammad N. Zahid ◽  
Marine Turek ◽  
Fei Xiao ◽  
Viet Loan Dao Thi ◽  
Maryse Guérin ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Scavenger Receptor ◽  
Type I ◽  
Hepatitis C Virus Infection ◽  
Viral Dissemination ◽  
Scavenger Receptor Class ◽  
Class B

scholarly journals Role of Scavenger Receptor Class B Type I in Hepatitis C Virus Entry: Kinetics and Molecular Determinants

2009 ◽  
Vol 84 (1) ◽  
pp. 34-43 ◽  
Author(s):  
Maria Teresa Catanese ◽  
Helenia Ansuini ◽  
Rita Graziani ◽  
Thierry Huby ◽  
Martine Moreau ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Entry ◽  
Scavenger Receptor ◽  
Specific Protein ◽  
Early Event ◽  
Type I ◽  
Direct Role ◽  
Scavenger Receptor Class ◽  
Class B

ABSTRACT Scavenger receptor class B type I (SR-BI) is an essential receptor for hepatitis C virus (HCV) and a cell surface high-density-lipoprotein (HDL) receptor. The mechanism of SR-BI-mediated HCV entry, however, is not clearly understood, and the specific protein determinants required for the recognition of the virus envelope are not known. HCV infection is strictly linked to lipoprotein metabolism, and HCV virions may initially interact with SR-BI through associated lipoproteins before subsequent direct interactions of the viral glycoproteins with SR-BI occur. The kinetics of inhibition of cell culture-derived HCV (HCVcc) infection with an anti-SR-BI monoclonal antibody imply that the recognition of SR-BI by HCV is an early event of the infection process. Swapping and single-substitution mutants between mouse and human SR-BI sequences showed reduced binding to the recombinant soluble E2 (sE2) envelope glycoprotein, thus suggesting that the SR-BI interaction with the HCV envelope is likely to involve species-specific protein elements. Most importantly, SR-BI mutants defective for sE2 binding, although retaining wild-type activity for receptor oligomerization and binding to the physiological ligand HDL, were impaired in their ability to fully restore HCVcc infectivity when transduced into an SR-BI-knocked-down Huh-7.5 cell line. These findings suggest a specific and direct role for the identified residues in binding HCV and mediating virus entry. Moreover, the observation that different regions of SR-BI are involved in HCV and HDL binding supports the hypothesis that new therapeutic strategies aimed at interfering with virus/SR-BI recognition are feasible.

Scavenger Receptor Class B Type I is a Hepatitis C Virus Receptor on Human Dendritic Cells

2006 ◽  
Vol 44 (01) ◽  
Author(s):  
H Barth ◽  
EK Schnober ◽  
C Neumann-Haefelin ◽  
R Thimme ◽  
HE Blum ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Scavenger Receptor ◽  
Type I ◽  
Virus Receptor ◽  
Scavenger Receptor Class ◽  
Class B ◽  
Human Dendritic Cells

scholarly journals Scavenger receptor class B type I is a key host factor for hepatitis C virus infection required for an entry step closely linked to CD81

Hepatology ◽  
2007 ◽  
Vol 46 (6) ◽  
pp. 1722-1731 ◽  
Author(s):  
Mirjam B. Zeisel ◽  
George Koutsoudakis ◽  
Eva K. Schnober ◽  
Anita Haberstroh ◽  
Hubert E. Blum ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Scavenger Receptor ◽  
Host Factor ◽  
Type I ◽  
Hepatitis C Virus Infection ◽  
Scavenger Receptor Class ◽  
Class B
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