scholarly journals Whole-genome sequencing in the investigation of recurrent invasive group A streptococcus outbreaks in a maternity unit

2019 ◽  
Vol 101 (3) ◽  
pp. 320-326 ◽  
Author(s):  
H. Dickinson ◽  
M. Reacher ◽  
B. Nazareth ◽  
H. Eagle ◽  
D. Fowler ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Group A Streptococcus ◽  
Whole Genome ◽  
Maternity Unit ◽  
Invasive Group ◽  
Group A

scholarly journals Clinical Manifestations and Bacterial Genomic Analysis of Group A Streptococcus Strains That Cause Pediatric Toxic Shock Syndrome

2018 ◽  
Vol 8 (3) ◽  
pp. 265-268
Author(s):  
Roman Deniskin ◽  
Brittany Shah ◽  
Flor M Muñoz ◽  
Anthony R Flores
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Toxic Shock Syndrome ◽  
Group A Streptococcus ◽  
Clinical Manifestations ◽  
Streptococcal Toxic Shock Syndrome ◽  
Whole Genome ◽  
Toxic Shock ◽  
Genetic Characteristics ◽  
Group A

Abstract We report here 18 cases of pediatric group A streptococcal toxic shock syndrome, associated clinical findings, and bacterial molecular genetic characteristics discovered through whole-genome sequencing. This comparative whole-genome sequencing revealed unique gene content (speK) and polymorphisms (dpiB) in emm87 group A Streptococcus, the relative contributions of which, in combination with the host response, in the development of streptococcal toxic shock syndrome remain to be elucidated.

scholarly journals Application of Whole-Genome Sequencing to an Unusual Outbreak of Invasive Group A Streptococcal Disease

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
Jessica Galloway-Peña ◽  
Meredith E. Clement ◽  
Batu K. Sharma Kuinkel ◽  
Felicia Ruffin ◽  
Anthony R. Flores ◽  
...  
Keyword(s):  
Point Source ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Genome Analysis ◽  
Whole Genome ◽  
Whole Genome Analysis ◽  
Invasive Group ◽  
Streptococcal Disease ◽  
Group A ◽  
Genome Analyses

Abstract Whole-genome analysis was applied to investigate atypical point-source transmission of 2 invasive group A streptococcal (GAS) infections. Isolates were serotype M4, ST39, and genetically indistinguishable. Comparison with MGAS10750 revealed nonsynonymous polymorphisms in ropB and increased speB transcription. This study demonstrates the usefulness of whole-genome analyses for GAS outbreaks.

scholarly journals A Protracted Outbreak of Invasive Group A Streptococcal Infection at a UK Long-Term Facility Investigated Using Whole Genome Sequencing

2016 ◽  
Vol 3 (suppl_1) ◽  
Author(s):  
Savita Gossain ◽  
Vicki Chalker ◽  
Georgia Kapatai ◽  
Juliana Coelho ◽  
Huda Mohamed ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Streptococcal Infection ◽  
Whole Genome ◽  
Invasive Group ◽  
Group A

scholarly journals Integrated whole-genome sequencing and temporospatial analysis of a continuing Group A Streptococcus epidemic

2013 ◽  
Vol 2 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Nahuel Fittipaldi ◽  
Gregory J Tyrrell ◽  
Donald E Low ◽  
Irene Martin ◽  
David Lin ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Group A Streptococcus ◽  
Whole Genome ◽  
Group A

scholarly journals Increase in Invasive Group A Streptococcal Disease and Emergence of Mucoid Strains in a Pediatric Population: February–June 2017

2019 ◽  
Vol 6 (7) ◽  
Author(s):  
Lorne W Walker ◽  
Lindsay Montoya ◽  
Sopio Chochua ◽  
Bernard Beall ◽  
Michael Green
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Hospital Admissions ◽  
Disease Incidence ◽  
Tertiary Care ◽  
Incidence Rates ◽  
Screening Tests ◽  
Whole Genome ◽  
Invasive Group ◽  
Group A

Abstract Background Infection with group A Streptococcus (GAS) can cause severe systemic and locally invasive disease. Invasive group A streptococcal (iGAS) disease incidence varies both seasonally and year-to-year, and it may exhibit clustered outbreaks. We observed an upswing in iGAS cases at a tertiary care Children’s Hospital, prompting further characterization of local iGAS disease. Methods Cases of iGAS disease were abstracted from the medical record by manual chart review of all positive screening tests and cultures for GAS over a 4-year span. Incidence rates per 1000 hospital admissions and per 100 positive GAS tests were calculated and compared. Selected isolates were further characterized by whole-genome sequencing. Results Significant year-to-year differences in per-admission iGAS incidence rate were observed in February and June, although per-positive test incidence rates were not significantly different. Whole-genome sequencing revealed 2 dominant serotypes—emm3 and emm6—with high rates of mucoid phenotype and systemic bacteremia. Conclusions We document a significant but transient increase in iGAS disease incidence in 2 months of 2017. Genome sequencing revealed 2 dominant serotypes associated with mucoid phenotypes and severe disease, highlighting the dynamic nature of iGAS disease pattern.

scholarly journals Severe emm89 Group A Streptococcal Disease Characterized by Toxic Shock and Endocarditis

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Morouge Alramadhan ◽  
Gloria P. Heresi ◽  
Anthony R. Flores
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Rare Case ◽  
Whole Genome ◽  
Toxic Shock ◽  
Invasive Group ◽  
Streptococcal Disease ◽  
Group A ◽  
Common Infections

Invasive group A Streptococcus infections are associated with diverse presentations. We report a severe, rare case of GAS infection with dissemination including endocarditis and STSS. While whole genome sequencing of blood and pharyngeal isolates did not reveal any unique features attributable to the severe presentation, our approach serves as a template for investigation of severe manifestations of common infections.

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