Anaerobic purification, characterization and preliminary mechanistic study of recombinant nitrous oxide reductase from Achromobacter cycloclastes

2007 ◽  
Vol 101 (11-12) ◽  
pp. 1836-1844 ◽  
Author(s):  
Koyu Fujita ◽  
Jeannine M. Chan ◽  
John A. Bollinger ◽  
Marcela L. Alvarez ◽  
David M. Dooley
Keyword(s):  
Nitrous Oxide ◽  
Mechanistic Study ◽  
Nitrous Oxide Reductase ◽  
Achromobacter Cycloclastes

Insight into Catalysis of Nitrous Oxide Reductase from High-resolution Structures of Resting and Inhibitor-bound Enzyme from Achromobacter cycloclastes

2006 ◽  
Vol 362 (1) ◽  
pp. 55-65 ◽  
Author(s):  
Konstantinos Paraskevopoulos ◽  
Svetlana V. Antonyuk ◽  
R. Gary Sawers ◽  
Robert R. Eady ◽  
S. Samar Hasnain
Keyword(s):  
Nitrous Oxide ◽  
High Resolution ◽  
Nitrous Oxide Reductase ◽  
Achromobacter Cycloclastes ◽  
Insight Into

Theoretically exploring the key role of the Lys412 residue in the conversion of N2O to N2by nitrous oxide reductase from Achromobacter cycloclastes

2015 ◽  
Vol 39 (10) ◽  
pp. 8093-8099 ◽  
Author(s):  
Hujun Xie ◽  
Chengcheng Liu ◽  
Xuelin Chen ◽  
Qunfang Lei ◽  
Wenjun Fang ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Nitrous Oxide Reductase ◽  
Achromobacter Cycloclastes ◽  
Back Donation

The active CuZcluster of NOR provides strong back-donation to coordinated N2O and activates the O atom of the N2O group facilitating H-bonding and protonationviathe Lys412 residue.

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