Insight into Catalysis of Nitrous Oxide Reductase from High-resolution Structures of Resting and Inhibitor-bound Enzyme from Achromobacter cycloclastes

2006 ◽  
Vol 362 (1) ◽  
pp. 55-65 ◽  
Author(s):  
Konstantinos Paraskevopoulos ◽  
Svetlana V. Antonyuk ◽  
R. Gary Sawers ◽  
Robert R. Eady ◽  
S. Samar Hasnain
Keyword(s):  
Nitrous Oxide ◽  
High Resolution ◽  
Nitrous Oxide Reductase ◽  
Achromobacter Cycloclastes ◽  
Insight Into

Anaerobic purification, characterization and preliminary mechanistic study of recombinant nitrous oxide reductase from Achromobacter cycloclastes

2007 ◽  
Vol 101 (11-12) ◽  
pp. 1836-1844 ◽  
Author(s):  
Koyu Fujita ◽  
Jeannine M. Chan ◽  
John A. Bollinger ◽  
Marcela L. Alvarez ◽  
David M. Dooley
Keyword(s):  
Nitrous Oxide ◽  
Mechanistic Study ◽  
Nitrous Oxide Reductase ◽  
Achromobacter Cycloclastes

scholarly journals Protonation state of the Cu4S2CuZsite in nitrous oxide reductase: redox dependence and insight into reactivity

2015 ◽  
Vol 6 (10) ◽  
pp. 5670-5679 ◽  
Author(s):  
Esther M. Johnston ◽  
Simone Dell'Acqua ◽  
Sofia R. Pauleta ◽  
Isabel Moura ◽  
Edward I. Solomon
Keyword(s):  
Electron Transfer ◽  
Nitrous Oxide ◽  
Redox State ◽  
Nitrous Oxide Reductase ◽  
Protonation State ◽  
Proton Coupled Electron Transfer ◽  
Insight Into

The edge ligand in the Cu4S2CuZform of nitrous oxide reductase is a μ2-thiolate in the 1-hole and a μ2-sulfide in the 2-hole redox state, leading to proton-coupled electron transfer reactivity.

scholarly journals A [3Cu:2S] cluster provides insight into the assembly and function of the CuZ site of nitrous oxide reductase

2021 ◽  
Author(s):  
Lin Zhang ◽  
Eckhard Bill ◽  
Peter M. H. Kroneck ◽  
Oliver Einsle
Keyword(s):  
Nitrous Oxide ◽  
Active Site ◽  
Structural Flexibility ◽  
Nitrous Oxide Reductase ◽  
Metal Site ◽  
And Function ◽  
Insight Into

Variants of all seven histidine ligands of the [4Cu:2S] active site of nitrous oxide reductase mostly result in loss of the metal site. However, a H382A variant retains a [3Cu:2S] cluster that hints towards a structural flexibility also present in the intact site.

Theoretically exploring the key role of the Lys412 residue in the conversion of N2O to N2by nitrous oxide reductase from Achromobacter cycloclastes

2015 ◽  
Vol 39 (10) ◽  
pp. 8093-8099 ◽  
Author(s):  
Hujun Xie ◽  
Chengcheng Liu ◽  
Xuelin Chen ◽  
Qunfang Lei ◽  
Wenjun Fang ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Nitrous Oxide Reductase ◽  
Achromobacter Cycloclastes ◽  
Back Donation

The active CuZcluster of NOR provides strong back-donation to coordinated N2O and activates the O atom of the N2O group facilitating H-bonding and protonationviathe Lys412 residue.

The nos (nitrous oxide reductase) gene cluster from the soil bacterium Achromobacter cycloclastes: Cloning, sequence analysis, and expression

1998 ◽  
Vol 70 (3-4) ◽  
pp. 155-169 ◽  
Author(s):  
Michele A McGuirl ◽  
Laura K. Nelson ◽  
John A Bollinger ◽  
Yiu-Kwok Chan ◽  
David M Dooley
Keyword(s):  
Nitrous Oxide ◽  
Sequence Analysis ◽  
Gene Cluster ◽  
Soil Bacterium ◽  
Nitrous Oxide Reductase ◽  
Reductase Gene ◽  
Achromobacter Cycloclastes

scholarly journals Spectroscopic Definition of the CuZ° Intermediate in Turnover of Nitrous Oxide Reductase and Molecular Insight into the Catalytic Mechanism

2017 ◽  
Vol 139 (12) ◽  
pp. 4462-4476 ◽  
Author(s):  
Esther M. Johnston ◽  
Cíntia Carreira ◽  
Simone Dell’Acqua ◽  
Somdatta Ghosh Dey ◽  
Sofia R. Pauleta ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Catalytic Mechanism ◽  
Nitrous Oxide Reductase ◽  
Definition Of ◽  
Insight Into

Skeletal elements of crinoid echinoderms: High-resolution structural and microanalytical results

1983 ◽  
Vol 41 ◽  
pp. 194-195
Author(s):  
D. F. Blake ◽  
L. F. Allard ◽  
D. R. Peacor
Keyword(s):  
High Resolution ◽  
Marine Invertebrates ◽  
Sea Urchins ◽  
Structural Features ◽  
Sea Stars ◽  
High Resolution Tem ◽  
Relationship Of ◽  
The Relationship ◽  
Insight Into

Echinodermata is a phylum of marine invertebrates which has been extant since Cambrian time (c.a. 500 m.y. before the present). Modern examples of echinoderms include sea urchins, sea stars, and sea lilies (crinoids). The endoskeletons of echinoderms are composed of plates or ossicles (Fig. 1) which are with few exceptions, porous, single crystals of high-magnesian calcite. Despite their single crystal nature, fracture surfaces do not exhibit the near-perfect {10.4} cleavage characteristic of inorganic calcite. This paradoxical mix of biogenic and inorganic features has prompted much recent work on echinoderm skeletal crystallography. Furthermore, fossil echinoderm hard parts comprise a volumetrically significant portion of some marine limestones sequences. The ultrastructural and microchemical characterization of modern skeletal material should lend insight into: 1). The nature of the biogenic processes involved, for example, the relationship of Mg heterogeneity to morphological and structural features in modern echinoderm material, and 2). The nature of the diagenetic changes undergone by their ancient, fossilized counterparts. In this study, high resolution TEM (HRTEM), high voltage TEM (HVTEM), and STEM microanalysis are used to characterize tha ultrastructural and microchemical composition of skeletal elements of the modern crinoid Neocrinus blakei.

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