Large pathogen screening reveals first report of Megaselia scalaris (Diptera: Phoridae) parasitizing Apis mellifera intermissa (Hymenoptera: Apidae)

Nosema apis and Nosema ceranae are the two main microsporidian parasites causing nosematosis in the honey bee Apis mellifera. The aim of the present study is to investigate the presence of Nosema apis and Nosema ceranae in the area of Bulgaria. The 16S (SSU) rDNA gene region was chosen for analysis. A duplex PCR assay was performed on 108 honey bee samples from three different parts of the country (South, North and West Bulgaria). The results showed that the samples from the northern part of the country were with the highest prevalence (77.2%) for Nosema ceranae while those from the mountainous parts (the Rodopa Mountains, South Bulgaria) were with the lowest rate (13.9%). Infection with Nosema apis alone and co-infection N. apis/N. ceranae were not detected in any samples. These findings suggest that Nosema ceranae is the dominant species in the Bulgarian honey bee. It is not known when the introduction of Nosema ceranae in Bulgaria has occurred, but as in the rest of the world, this species has become the dominant one in Bulgarian Apis mellifera. In conclusion, this is the first report for molecular detection of Nosema infection of honey bee in Bulgaria. The results showed that N. ceranae is the main Nosema species in Bulgaria.

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Complete mitochondrial genome of the Algerian honeybee,Apis mellifera intermissa(Hymenoptera: Apidae)

Mitochondrial DNA ◽

10.3109/19401736.2014.963815 ◽

2014 ◽

pp. 1-2 ◽

Cited By ~ 3

Author(s):

Peng Hu ◽

Zhi-Xiang Lu ◽

Nizar Haddad ◽

Adjlane Noureddine ◽

Wahida Loucif-Ayad ◽

...

Keyword(s):

Apis Mellifera ◽

Mitochondrial Genome ◽

Complete Mitochondrial Genome ◽

Apis Mellifera Intermissa

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Etude de la Diversité Morphométrique de Deux Populations d’Abeilles Domestiques ( Apis Mellifera Intermissa et Apis Mellifera Sahariensis ) du Sud Algérien

Synthèse Revue des Sciences et de la Technologie ◽

10.12816/0027832 ◽

2014 ◽

pp. 84-95

Author(s):

Hassina Bendjedid ◽

Mohamed Achou

Keyword(s):

Apis Mellifera ◽

Apis Mellifera Intermissa ◽

Apis Mellifera Sahariensis

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The first report of the prevalence of Nosema ceranae in Bulgaria

10.7287/peerj.preprints.3342v1 ◽

2017 ◽

Author(s):

Peter Hristov ◽

Any Georgieva ◽

Georgi Radoslavov ◽

Daniela Sirakova ◽

Gyulnas Dzhebir ◽

...

Keyword(s):

Apis Mellifera ◽

Honey Bee ◽

Molecular Detection ◽

Nosema Ceranae ◽

Nosema Apis ◽

Pcr Assay ◽

First Report ◽

Duplex Pcr Assay ◽

Different Parts ◽

Rate Of Invasion

Nosema apis and Nosema ceranae are the two main microsporidian parasites causing nosematosis in honey bee Apis mellifera. The object of the present study is to investigate the presence of Nosema apis and Nosema ceranae in the Bulgarian honey bee. The 16S (SSU) rDNA gene region was chosen for analysis. A duplex PCR assay was performed on 108 honey bee samples from three different parts of the country (South, North and West Bulgaria). The results showed that the samples from the northern part of the country were with the highest rate of invasion (77.2%) for Nosema ceranae while those from the mountainous parts (the Rodopa Mountains, South Bulgaria) were with the lowest rate (13.9%). Infection with Nosema apis alone and co-infection N. apis/N. ceranae were not detected in any samples. These findings suggest that Nosema ceranae is the dominant species in the Bulgarian honey bee. It is not known when the introduction of Nosema ceranae in Bulgaria has occurred, but like in the rest of the world, this species has become the dominant one in Bulgarian Apis mellifera. In conclusion, this is the first report for molecular detection of Nosema infection in Bulgarian honey bee that confirms the worldwide dissemination and prevalence of Nosema ceranae.

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Bioactivité de quelques huiles essentielles par l’évaluation de l’effet acaricide vis-à-vis de la varroase et incidence sur la qualité du miel

Phytothérapie ◽

10.3166/phyto-2021-0270 ◽

2021 ◽

Author(s):

H. Hacib ◽

Z. Berkani-Ghalem

Keyword(s):

Apis Mellifera ◽

Varroa Destructor ◽

Rosmarinus Officinalis ◽

Huiles Essentielles ◽

Laurus Nobilis ◽

Apis Mellifera Intermissa

Le parasite Varroa destructor de l’abeille domestique Apis mellifera intermissa est actuellement considéré comme la plus grande menace pour cet insecte et responsable d’une partie importante des mortalités des colonies d’abeilles qu’il infeste. Pour cela, des traitements « biologiques » se sont avérés très efficaces, et leurs résidus présentent moins de nocivité pour la santé de l’être humain. Les huiles essentielles de Rosmarinus officinalis (lot 1), Laurus nobilis (lot 2) et Mentha viridis (lot 3) extraites par hydrodistillation ont été étudiées sur Varroa destructor. Puis, une analyse des miels a été initiée pour déterminer la présence des huiles utilisées. Il ressort des résultats obtenus qu’aucun d’entre eux n’a donné pleinement 100 % d’efficacité à cause de la présence du couvain, mais il ressort clairement que les trois huiles essentielles ont manifesté des résultats appréciables et semblent avoir grandement diminué le taux d’infestation final pour atteindre 0,30 % pour le romarin, 0,6 % pour le laurier et 0,29 % pour la menthe, ce qui n’est pas négligeable. Le lot témoin (lot 4), non traité, a eu une mortalité naturelle de varroa assez importante, le taux d’infestation est toujours élevé, il est de 22,1 % pendant la durée des traitements. Les résultats obtenus pour l’analyse des lipides nous ont permis de constater que nos miels répondent aux normes. Les teneurs en lipides sont très faibles, et les abeilles n’ont pas utilisé l’huile de traitement dans l’élaboration du miel. À cet effet, nos analyses ont confirmé l’absence d’huile essentielle dans ce produit de la ruche.

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