scholarly journals The first report of the prevalence of Nosema ceranae in Bulgaria

2017 ◽  
Author(s):  
Peter Hristov ◽  
Any Georgieva ◽  
Georgi Radoslavov ◽  
Daniela Sirakova ◽  
Gyulnas Dzhebir ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Molecular Detection ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Pcr Assay ◽  
First Report ◽  
Duplex Pcr Assay ◽  
Different Parts ◽  
Rate Of Invasion

Nosema apis and Nosema ceranae are the two main microsporidian parasites causing nosematosis in honey bee Apis mellifera. The object of the present study is to investigate the presence of Nosema apis and Nosema ceranae in the Bulgarian honey bee. The 16S (SSU) rDNA gene region was chosen for analysis. A duplex PCR assay was performed on 108 honey bee samples from three different parts of the country (South, North and West Bulgaria). The results showed that the samples from the northern part of the country were with the highest rate of invasion (77.2%) for Nosema ceranae while those from the mountainous parts (the Rodopa Mountains, South Bulgaria) were with the lowest rate (13.9%). Infection with Nosema apis alone and co-infection N. apis/N. ceranae were not detected in any samples. These findings suggest that Nosema ceranae is the dominant species in the Bulgarian honey bee. It is not known when the introduction of Nosema ceranae in Bulgaria has occurred, but like in the rest of the world, this species has become the dominant one in Bulgarian Apis mellifera. In conclusion, this is the first report for molecular detection of Nosema infection in Bulgarian honey bee that confirms the worldwide dissemination and prevalence of Nosema ceranae.

scholarly journals The first report of the prevalence of Nosema ceranae in Bulgaria

2017 ◽  
Author(s):  
Peter Hristov ◽  
Any Georgieva ◽  
Georgi Radoslavov ◽  
Daniela Sirakova ◽  
Gyulnas Dzhebir ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Molecular Detection ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Pcr Assay ◽  
First Report ◽  
Duplex Pcr Assay ◽  
Different Parts ◽  
Rate Of Invasion

Nosema apis and Nosema ceranae are the two main microsporidian parasites causing nosematosis in honey bee Apis mellifera. The object of the present study is to investigate the presence of Nosema apis and Nosema ceranae in the Bulgarian honey bee. The 16S (SSU) rDNA gene region was chosen for analysis. A duplex PCR assay was performed on 108 honey bee samples from three different parts of the country (South, North and West Bulgaria). The results showed that the samples from the northern part of the country were with the highest rate of invasion (77.2%) for Nosema ceranae while those from the mountainous parts (the Rodopa Mountains, South Bulgaria) were with the lowest rate (13.9%). Infection with Nosema apis alone and co-infection N. apis/N. ceranae were not detected in any samples. These findings suggest that Nosema ceranae is the dominant species in the Bulgarian honey bee. It is not known when the introduction of Nosema ceranae in Bulgaria has occurred, but like in the rest of the world, this species has become the dominant one in Bulgarian Apis mellifera. In conclusion, this is the first report for molecular detection of Nosema infection in Bulgarian honey bee that confirms the worldwide dissemination and prevalence of Nosema ceranae.

scholarly journals The first report of the prevalence of Nosema ceranae in Bulgaria

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4252 ◽  
Author(s):  
Rositsa Shumkova ◽  
Ani Georgieva ◽  
Georgi Radoslavov ◽  
Daniela Sirakova ◽  
Gyulnas Dzhebir ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Molecular Detection ◽  
Ssu Rdna ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Pcr Assay ◽  
First Report ◽  
Duplex Pcr Assay ◽  
Different Parts

Nosema apis and Nosema ceranae are the two main microsporidian parasites causing nosematosis in the honey bee Apis mellifera. The aim of the present study is to investigate the presence of Nosema apis and Nosema ceranae in the area of Bulgaria. The 16S (SSU) rDNA gene region was chosen for analysis. A duplex PCR assay was performed on 108 honey bee samples from three different parts of the country (South, North and West Bulgaria). The results showed that the samples from the northern part of the country were with the highest prevalence (77.2%) for Nosema ceranae while those from the mountainous parts (the Rodopa Mountains, South Bulgaria) were with the lowest rate (13.9%). Infection with Nosema apis alone and co-infection N. apis/N. ceranae were not detected in any samples. These findings suggest that Nosema ceranae is the dominant species in the Bulgarian honey bee. It is not known when the introduction of Nosema ceranae in Bulgaria has occurred, but as in the rest of the world, this species has become the dominant one in Bulgarian Apis mellifera. In conclusion, this is the first report for molecular detection of Nosema infection of honey bee in Bulgaria. The results showed that N. ceranae is the main Nosema species in Bulgaria.

scholarly journals Molecular Detection of Nosema spp. in Honey in Bulgaria

2021 ◽  
Vol 9 (1) ◽  
pp. 10
Author(s):  
Delka Salkova ◽  
Rositsa Shumkova ◽  
Ralitsa Balkanska ◽  
Nadezhda Palova ◽  
Boyko Neov ◽  
...  
Keyword(s):  
Honey Bee ◽  
Varroa Destructor ◽  
Environmental Samples ◽  
Molecular Detection ◽  
Genetic Material ◽  
Environmental Dna ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Varroa Mite ◽  
Exogenous Dna

Environmental DNA (eDNA) analysis is related to screening genetic material of various organisms in environmental samples. Honey represents a natural source of exogenous DNA, which allows for the detection of different honey bee pathogens and parasites. In the present study, we extracted DNA from 20 honey samples from different regions in Bulgaria and tested for the presence of DNA of the ectoparasitic mite Varroa destructor, as well as Nosema apis and Nosema ceranae. Only Nosema ceranae was detected, showing up in 30% of all samples, which confirms the widespread prevalence of this pathogen. All positive samples were found in plain regions of the country, while this pathogen was not detected in mountainous parts. None of the samples gave positive amplifications for the Nosema apis and Varroa mite. The obtained results from this study confirm previous observations that eDNA contained in honey is a potent source for effective biomonitoring of actual diseases in the honey bee.

scholarly journals Infra-Population and -Community Dynamics of the Parasites Nosema apis and Nosema ceranae, and Consequences for Honey Bee (Apis mellifera) Hosts

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e99465 ◽  
Author(s):  
Geoffrey R. Williams ◽  
Dave Shutler ◽  
Karen L. Burgher-MacLellan ◽  
Richard E. L. Rogers
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Community Dynamics ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Population And Community Dynamics

scholarly journals Variation in the Distribution of Nosema Species in Honeybees (Apis mellifera Linnaeus) between the Neighboring Countries Estonia and Latvia

2021 ◽  
Vol 8 (4) ◽  
pp. 58
Author(s):  
Sigmar Naudi ◽  
Juris Šteiselis ◽  
Margret Jürison ◽  
Risto Raimets ◽  
Lea Tummeleht ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Disease Control ◽  
Honey Bee ◽  
Species Distribution ◽  
Stress Responses ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Behavioral Stress ◽  
Nosema Species ◽  
Honey Yield

The unicellular spore-forming parasites Nosema apis and Nosema ceranae are considered to be one of the causes of increased honey bee mortality in recent years. These pathogens attack their honey bee hosts through their gut, causing changes in behavioral stress responses and possibly resulting in decreased honey yield and increased honey bee mortality. The present study aimed to determine the prevalence of Nosema spp. (nosemosis) in Estonia and Latvia, as well as the persistence of the disease in previously infected hives. Currently, N. ceranae is considered the most virulent species and is predominant worldwide. However, in some regions, usually with colder climates, N. apis is still prevalent. To achieve better disease control, it is important to determine the species distribution. For this purpose, we selected 30 apiaries in Estonia and 60 in Latvia that were positive for Nosema spp. in the EPILOBEE (2012–2014) study, which was 5 years prior to the present study. The results show that, while both species are present in Estonia and Latvia, N. apis is dominant in Estonia (43%), and N. ceranae is dominant in Latvia (47%). We also found that the pathogens are very persistent, since 5 years later, only 33% of infected apiaries in Estonia and 20% of infected apiaries in Latvia, we could not detect any pathogens at the time of sampling.

Survey of feral honey bee (Apis mellifera) colonies for Nosema apis in Western Australia

2007 ◽  
Vol 47 (7) ◽  
pp. 883 ◽  
Author(s):  
Rob Manning ◽  
Kate Lancaster ◽  
April Rutkay ◽  
Linda Eaton
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Western Australia ◽  
Honey Bees ◽  
Nosema Apis ◽  
The South ◽  
South West ◽  
Feral Populations ◽  
Significant Difference

The parasite, Nosema apis, was found to be widespread among feral populations of honey bees (Apis mellifera) in the south-west of Western Australia. The location, month of collection and whether the feral colony was enclosed in an object or exposed to the environment, all affected the presence and severity of infection. There was no significant difference in the probability of infection between managed and feral bees. However, when infected by N. apis, managed bees appeared to have a greater severity of the infection.

scholarly journals Physiological effects of the interaction between Nosema ceranae and sequential and overlapping exposure to glyphosate and difenoconazole in the honey bee Apis mellifera

2021 ◽  
Vol 217 ◽  
pp. 112258
Author(s):  
Hanine Almasri ◽  
Daiana Antonia Tavares ◽  
Marie Diogon ◽  
Maryline Pioz ◽  
Maryam Alamil ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Nosema Ceranae ◽  
Physiological Effects

scholarly journals Dynamics of Nosema apis and Nosema ceranae Co-Infection Seasonally in Honey Bee (Apis mellifera L.) Colonies

2019 ◽  
Vol 63 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Asli Özkırım ◽  
Aygün Schiesser ◽  
Nevіn Keskin
Keyword(s):  
Apis Mellifera ◽  
Apis Cerana ◽  
Winter Season ◽  
Nosema Ceranae ◽  
Molecular Techniques ◽  
Winter Period ◽  
Nosema Apis ◽  
Infection Level ◽  
Seasonal Infection ◽  
Asian Honeybee

AbstractNosema apis is a pathogen spesific for the European honeybee, Apis mellifera L., while Nosema ceranae is specific for the Asian honeybee, Apis cerana. Turkey provides different environmental and host conditions for both Nosema species. The aim of the study is to determine the dynamic of N. cerenae and N. apis seasonal infection. A number of samples were collected from different apiaries between 2009-2016 years. The samples were kept at −20°C in the laboratory. Light microscopy was used for spore counting and molecular techniques were used to identify the Nosema species. The results showed that winter season had an impact on the type of Nosema as well as on infection rates. The number of N. ceranae spores decreases significantly at low temperatures (≤ 5°C). The winter period was found to be the main factor affecting nosema infection level and dominancy of Nosema ceranae. Furthermore, co-infection of both species is an indicator of the dynamics of N. apis and N. ceranae. This study suggests, that there is a dynamic prevalence among the Nosema species depending of the average winter temperature and not a replacement of N. apis by N. ceranae.

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