scholarly journals Purification and characterization of carboxymethylcellulase from Bacillus pumilus EWBCM1 isolated from earthworm gut (Eudrilus eugeniae)

2021 ◽  
Vol 33 (1) ◽  
pp. 101261
Author(s):  
Thiruvengadam Shankar ◽  
Subbiah Sankaralingam ◽  
Chellapandian Balachandran ◽  
Arunachalam Chinnathambi ◽  
Omaima Nasif ◽  
...  
Keyword(s):  
Bacillus Pumilus ◽  
Eudrilus Eugeniae ◽  
Purification And Characterization ◽  
Earthworm Gut

Purification and Characterization of an Acetyl Xylan Esterase from Bacillus pumilus

1998 ◽  
Vol 64 (2) ◽  
pp. 789-792 ◽  
Author(s):  
Giuliano Degrassi ◽  
Benedict C. Okeke ◽  
Carlo V. Bruschi ◽  
Vittorio Venturi
Keyword(s):  
Gel Electrophoresis ◽  
Bacillus Pumilus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Acetyl Xylan Esterase ◽  
Purification And Characterization ◽  
Michaelis Constant ◽  
Naphthyl Acetate

ABSTRACT Bacillus pumilus PS213 was found to be able to release acetate from acetylated xylan. The enzyme catalyzing this reaction has been purified to homogeneity and characterized. The enzyme was secreted, and its production was induced by corncob powder and xylan. Its molecular mass, as determined by gel filtration, is 190 kDa, while sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed a single band of 40 kDa. The isoelectric point was found to be 4.8, and the enzyme activity was optimal at 55°C and pH 8.0. The activity was inhibited by most of the metal ions, while no enhancement was observed. The Michaelis constant (Km ) andV max for α-naphthyl acetate were 1.54 mM and 360 μmol min−1 mg of protein−1, respectively.

Purification and characterization of a new bioscouring pectate lyase from Bacillus pumilus BK2

2006 ◽  
Vol 121 (3) ◽  
pp. 390-401 ◽  
Author(s):  
Barbara G. Klug-Santner ◽  
Wolfgang Schnitzhofer ◽  
Maria Vršanská ◽  
Jörg Weber ◽  
Pramod B. Agrawal ◽  
...  
Keyword(s):  
Bacillus Pumilus ◽  
Pectate Lyase ◽  
Purification And Characterization

Isolation, purification, and characterization of haloalkaline xylanase from a marine Bacillus pumilus strain, GESF-1

2010 ◽  
Vol 15 (6) ◽  
pp. 998-1005 ◽  
Author(s):  
Gopalakrishnan Menon ◽  
Kalpana Mody ◽  
Jitendra Keshri ◽  
Bhavanath Jha
Keyword(s):  
Bacillus Pumilus ◽  
Purification And Characterization

scholarly journals Purification and Characterization of a Fibrinolytic Enzyme from Bacillus pumilus 2.g Isolated from Gembus, an Indonesian Fermented Food

2014 ◽  
Vol 19 (3) ◽  
pp. 213-219 ◽  
Author(s):  
Diana Nur Afifah ◽  
Muhammad Sulchan ◽  
Dahrul Syah ◽  
Yanti Yanti ◽  
Maggy Thenawidjaja Suhartono ◽  
...  
Keyword(s):  
Bacillus Pumilus ◽  
Fermented Food ◽  
Fibrinolytic Enzyme ◽  
Purification And Characterization

Purification and Characterization of a Novel Collagenase from Bacillus pumilus Col-J

2009 ◽  
Vol 160 (1) ◽  
pp. 129-139 ◽  
Author(s):  
Qi Wu ◽  
Chen Li ◽  
Chenglei Li ◽  
Hui Chen ◽  
Liu Shuliang
Keyword(s):  
Bacillus Pumilus ◽  
Purification And Characterization
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