Purification and characterization of a new bioscouring pectate lyase from Bacillus pumilus BK2

2006 ◽  
Vol 121 (3) ◽  
pp. 390-401 ◽  
Author(s):  
Barbara G. Klug-Santner ◽  
Wolfgang Schnitzhofer ◽  
Maria Vršanská ◽  
Jörg Weber ◽  
Pramod B. Agrawal ◽  
...  
Keyword(s):  
Bacillus Pumilus ◽  
Pectate Lyase ◽  
Purification And Characterization

scholarly journals Purification and characterization of carboxymethylcellulase from Bacillus pumilus EWBCM1 isolated from earthworm gut (Eudrilus eugeniae)

2021 ◽  
Vol 33 (1) ◽  
pp. 101261
Author(s):  
Thiruvengadam Shankar ◽  
Subbiah Sankaralingam ◽  
Chellapandian Balachandran ◽  
Arunachalam Chinnathambi ◽  
Omaima Nasif ◽  
...  
Keyword(s):  
Bacillus Pumilus ◽  
Eudrilus Eugeniae ◽  
Purification And Characterization ◽  
Earthworm Gut

Purification and Characterization of an Acetyl Xylan Esterase from Bacillus pumilus

1998 ◽  
Vol 64 (2) ◽  
pp. 789-792 ◽  
Author(s):  
Giuliano Degrassi ◽  
Benedict C. Okeke ◽  
Carlo V. Bruschi ◽  
Vittorio Venturi
Keyword(s):  
Gel Electrophoresis ◽  
Bacillus Pumilus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Acetyl Xylan Esterase ◽  
Purification And Characterization ◽  
Michaelis Constant ◽  
Naphthyl Acetate

ABSTRACT Bacillus pumilus PS213 was found to be able to release acetate from acetylated xylan. The enzyme catalyzing this reaction has been purified to homogeneity and characterized. The enzyme was secreted, and its production was induced by corncob powder and xylan. Its molecular mass, as determined by gel filtration, is 190 kDa, while sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed a single band of 40 kDa. The isoelectric point was found to be 4.8, and the enzyme activity was optimal at 55°C and pH 8.0. The activity was inhibited by most of the metal ions, while no enhancement was observed. The Michaelis constant (Km ) andV max for α-naphthyl acetate were 1.54 mM and 360 μmol min−1 mg of protein−1, respectively.

Thermodynamic characterization of a highly thermoactive extracellular pectate lyase from a new isolate Bacillus pumilus DKS1

2008 ◽  
Vol 99 (17) ◽  
pp. 8088-8094 ◽  
Author(s):  
Snehasish Basu ◽  
Abhrajyoti Ghosh ◽  
Amit Bera ◽  
Manabendra N. Saha ◽  
Dhrubajyoti Chattopadhyay ◽  
...  
Keyword(s):  
Bacillus Pumilus ◽  
Pectate Lyase ◽  
Thermodynamic Characterization ◽  
Bacillus Pumilus Dks1
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