Hybrid Thermophilic/Mesophilic Enzymes Reveal a Role for Conformational Disorder in Regulation of Bacterial Enzyme I

Journal of Molecular Biology ◽

10.1016/j.jmb.2020.05.024 ◽

2020 ◽

Vol 432 (16) ◽

pp. 4481-4498 ◽

Author(s):

Rochelle R. Dotas ◽

Trang T. Nguyen ◽

Charles E. Stewart ◽

Rodolfo Ghirlando ◽

Davit A. Potoyan ◽

...

Keyword(s):

Conformational Disorder ◽

Bacterial Enzyme ◽

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An allosteric pocket for inhibition of bacterial Enzyme I identified by NMR-based fragment screening

Journal of Structural Biology X ◽

10.1016/j.yjsbx.2020.100034 ◽

2020 ◽

Vol 4 ◽

pp. 100034

Author(s):

Trang T. Nguyen ◽

Vincenzo Venditti

Keyword(s):

Fragment Screening ◽

Bacterial Enzyme ◽

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Large interdomain rearrangement triggered by suppression of micro- to millisecond dynamics in bacterial Enzyme I

Nature Communications ◽

10.1038/ncomms6960 ◽

2015 ◽

Vol 6 (1) ◽

Author(s):

Vincenzo Venditti ◽

Vitali Tugarinov ◽

Charles D. Schwieters ◽

Alexander Grishaev ◽

G. Marius Clore

Keyword(s):

Bacterial Enzyme ◽

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Phospholipid-requiring bacterial enzyme. I. Purification and properties of uridine diphosphate galactose: lipopolysaccharide α-3-galactosyl transferase

Biochemistry ◽

10.1021/bi00837a003 ◽

1969 ◽

Vol 8 (9) ◽

pp. 3500-3507 ◽

Author(s):

Akira Endo ◽

Lawrence Rothfield

Keyword(s):

Uridine Diphosphate ◽

Galactosyl Transferase ◽

Bacterial Enzyme ◽

Purification And Properties ◽

Enzyme I ◽

Uridine Diphosphate Galactose

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The oligomerization state of bacterial enzyme I (EI) determines EI's allosteric stimulation or competitive inhibition by α-ketoglutarate

Journal of Biological Chemistry ◽

10.1074/jbc.ra117.001466 ◽

2018 ◽

Vol 293 (7) ◽

pp. 2631-2639 ◽

Author(s):

Trang T. Nguyen ◽

Rodolfo Ghirlando ◽

Vincenzo Venditti

Keyword(s):

Competitive Inhibition ◽

Bacterial Enzyme ◽

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N-terminal fusion of the N-terminal domain of bacterial enzyme I facilitates recombinant expression and purification of the human RNA demethylases FTO and Alkbh5

Protein Expression and Purification ◽

10.1016/j.pep.2019.105540 ◽

2020 ◽

Vol 167 ◽

pp. 105540

Author(s):

Balabhadra Khatiwada ◽

Jeffrey A. Purslow ◽

Eric S. Underbakke ◽

Vincenzo Venditti

Keyword(s):

Recombinant Expression ◽

Expression And Purification ◽

Terminal Domain ◽

Bacterial Enzyme ◽

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Allosteric regulation of bacterial Enzyme I: Toward the discovery of a new class of antimicrobial compounds

10.31274/etd-20210609-133 ◽

2021 ◽

Author(s):

Trang Truc Nguyen

Keyword(s):

Allosteric Regulation ◽

Antimicrobial Compounds ◽

New Class ◽

Bacterial Enzyme ◽

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Characterization of the C-terminal binding domain from bacterial Enzyme I

10.31274/etd-20200624-160 ◽

2020 ◽

Author(s):

Rochelle Rea Dotas

Keyword(s):

Binding Domain ◽

Bacterial Enzyme ◽

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Faculty Opinions recommendation of Polyphosphate kinase 1, a conserved bacterial enzyme, in a eukaryote, Dictyostelium discoideum, with a role in cytokinesis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1092338.546759 ◽

2007 ◽

Author(s):

Roberto Docampo

Keyword(s):

Dictyostelium Discoideum ◽

Polyphosphate Kinase ◽

Bacterial Enzyme

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Faculty Opinions recommendation of The bacterial enzyme RppH triggers messenger RNA degradation by 5' pyrophosphate removal.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1097848.555381 ◽

2008 ◽

Author(s):

Stephen Busby

Keyword(s):

Messenger Rna ◽

Rna Degradation ◽

Bacterial Enzyme

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Faculty Opinions recommendation of IgG glycan hydrolysis by a bacterial enzyme as a therapy against autoimmune conditions.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1104150.560144 ◽

2008 ◽

Author(s):

Victor Nizet

Keyword(s):

Bacterial Enzyme ◽

Autoimmune Conditions

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