Molecular characterization of Group A streptococcal isolates causing scarlet fever and pharyngitis among young children: A retrospective study from a northern Taiwan medical center

Group A rotaviruses (RVAs) infect a wide variety of mammalian and avian species. Animals act as a potential reservoir to RVA human infections by direct virion transmission or by contributing genes to reassortants. Here, we report the molecular characterization of a rare human RVA strain Ni17-46 with a genotype G15P[14], isolated in Japan in 2017 during rotavirus surveillance in a paediatric outpatient clinic. The genome constellation of this strain was G15-P[14]-I2-R2-C2-M2-A13-N2-T9-E2-H3. This is the first report of an RVA with G15 genotype in humans, and sequencing and phylogenetic analysis results suggest that human infection with this strain has zoonotic origin from the bovine species. Given the fact that this strain was isolated from a patient with gastroenteritis and dehydration symptoms, we must take into account the virulence of this strain in humans.

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MBCL-08. MOLECULAR CHARACTERIZATION OF NODULAR DESMOPLASTIC MEDULLOBLASTOMAS IN YOUNG CHILDREN TREATED ON ACNS1221. A REPORT FROM THE CHILDREN ONCOLOGY GROUP

Neuro-Oncology ◽

10.1093/neuonc/noy059.406 ◽

2018 ◽

Vol 20 (suppl_2) ◽

pp. i118-i119 ◽

Cited By ~ 2

Author(s):

Lucie Lafay-Cousin ◽

Giles Robinson ◽

Vasilisa Rudneva ◽

Paul Northcott ◽

Catherine Billups ◽

...

Keyword(s):

Young Children ◽

Molecular Characterization ◽

Oncology Group

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Epidemiology, clinical signs, histopathology and molecular characterization of canine leproid granuloma: a retrospective study of cases from Brazil

Veterinary Dermatology ◽

10.1111/j.1365-3164.2010.00934.x ◽

2011 ◽

Vol 22 (3) ◽

pp. 249-256 ◽

Cited By ~ 10

Author(s):

Lissandro Gonçalves Conceição ◽

Livia Maria Rosa Acha ◽

Alexandre Secorun Borges ◽

Fernanda G. Assis ◽

Fabricia Hallack Loures ◽

...

Keyword(s):

Retrospective Study ◽

Molecular Characterization ◽

Clinical Signs

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Molecular epidemiology of group A streptococcus causing scarlet fever in northern Taiwan, 2001–2002

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2007.01.013 ◽

2007 ◽

Vol 58 (3) ◽

pp. 289-295 ◽

Cited By ~ 14

Author(s):

Ying-Yan Chen ◽

Chung-Ter Huang ◽

Shu-Man Yao ◽

Yi-Ching Chang ◽

Pei-Wun Shen ◽

...

Keyword(s):

Molecular Epidemiology ◽

Scarlet Fever ◽

Group A Streptococcus ◽

Group A ◽

Northern Taiwan

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Prophage exotoxins enhance colonization fitness in epidemic scarlet fever-causing Streptococcus pyogenes

Nature Communications ◽

10.1038/s41467-020-18700-5 ◽

2020 ◽

Vol 11 (1) ◽

Author(s):

Stephan Brouwer ◽

Timothy C. Barnett ◽

Diane Ly ◽

Katherine J. Kasper ◽

David M. P. De Oliveira ◽

...

Keyword(s):

Streptococcus Pyogenes ◽

Scarlet Fever ◽

Global Public Health ◽

Nasopharyngeal Colonization ◽

North East ◽

Streptolysin O ◽

Knockout Mutants ◽

Group A ◽

Bacterial Superantigen

Abstract The re-emergence of scarlet fever poses a new global public health threat. The capacity of North-East Asian serotype M12 (emm12) Streptococcus pyogenes (group A Streptococcus, GAS) to cause scarlet fever has been linked epidemiologically to the presence of novel prophages, including prophage ΦHKU.vir encoding the secreted superantigens SSA and SpeC and the DNase Spd1. Here, we report the molecular characterization of ΦHKU.vir-encoded exotoxins. We demonstrate that streptolysin O (SLO)-induced glutathione efflux from host cellular stores is a previously unappreciated GAS virulence mechanism that promotes SSA release and activity, representing the first description of a thiol-activated bacterial superantigen. Spd1 is required for resistance to neutrophil killing. Investigating single, double and triple isogenic knockout mutants of the ΦHKU.vir-encoded exotoxins, we find that SpeC and Spd1 act synergistically to facilitate nasopharyngeal colonization in a mouse model. These results offer insight into the pathogenesis of scarlet fever-causing GAS mediated by prophage ΦHKU.vir exotoxins.

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