scholarly journals Epidemiology, clinical signs, histopathology and molecular characterization of canine leproid granuloma: a retrospective study of cases from Brazil

2011 ◽  
Vol 22 (3) ◽  
pp. 249-256 ◽  
Author(s):  
Lissandro Gonçalves Conceição ◽  
Livia Maria Rosa Acha ◽  
Alexandre Secorun Borges ◽  
Fernanda G. Assis ◽  
Fabricia Hallack Loures ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Molecular Characterization ◽  
Clinical Signs

scholarly journals Molecular characterization and distribution of Plasmodium matutinum, a common avian malaria parasite

Parasitology ◽  
2017 ◽  
Vol 144 (13) ◽  
pp. 1726-1735 ◽  
Author(s):  
GEDIMINAS VALKIŪNAS ◽  
MIKAS ILGŪNAS ◽  
DOVILĖ BUKAUSKAITĖ ◽  
VAIDAS PALINAUSKAS ◽  
RASA BERNOTIENĖ ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Molecular Characterization ◽  
Malaria Parasite ◽  
Avian Malaria ◽  
Clinical Signs ◽  
Molecular Data ◽  
Infected Blood ◽  
Avian Malaria Parasite ◽  
Thrush Nightingale

SUMMARYSpecies of Plasmodium (Plasmodiidae, Haemosporida) are widespread and cause malaria, which can be severe in avian hosts. Molecular markers are essential to detect and identify parasites, but still absent for many avian malaria and related haemosporidian species. Here, we provide first molecular characterization of Plasmodium matutinum, a common agent of avian malaria. This parasite was isolated from a naturally infected thrush nightingale Luscinia luscinia (Muscicapidae). Fragments of mitochondrial, apicoplast and nuclear genomes were obtained. Domestic canaries Serinus canaria were susceptible after inoculation of infected blood, and the long-lasting light parasitemia developed in two exposed birds. Clinical signs of illness were not reported. Illustrations of blood stages of P. matutinum (pLINN1) are given, and phylogenetic analysis identified the closely related avian Plasmodium species. The phylogeny based on partial cytochrome b (cyt b) sequences suggests that this parasite is most closely related to Plasmodium tejerai (cyt b lineage pSPMAG01), a common malaria parasite of American birds. Both these parasites belong to subgenus Haemamoeba, and their blood stages are similar morphologically, particularly due to marked vacuolization of the cytoplasm in growing erythrocytic meronts. Molecular data show that transmission of P. matutinum (pLINN1) occurs broadly in the Holarctic, and the parasite likely is of cosmopolitan distribution. Passeriform birds and Culex mosquitoes are common hosts. This study provides first molecular markers for detection of P. matutinum.

scholarly journals Isolation and Molecular Characterization of Aspergillus fumigatus and Aspergillus flavus Isolated from Poultry Birds in Ado- Ekiti, Nigeria

2020 ◽  
pp. 31-44
Author(s):  
E. D. Fagbohun ◽  
K. J. Ayantola ◽  
A. J. Toyin-Famoroti
Keyword(s):  
Aspergillus Fumigatus ◽  
Molecular Characterization ◽  
Aspergillus Flavus ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Aspergillus Species ◽  
Exercise Intolerance ◽  
State University ◽  
Poultry Farms

Aim: The study was carried out to isolate and identify Aspergillus species from commercial birds with suspected aspergillosis in the poultry farms within Ado Ekiti metropolis Nigeria.  Place and Period of Study: The study was carried out in the Department of Microbiology, Faculty of Science, Ekiti State University Ado Ekiti, Nigeria in August 2016. Methodology: A total of 35 sick/suspected birds were collected randomly from three poultry farms. At Ago-Aduloju poultry farms, 15 samples were randomly collected from 1000 birds while at Ekiti State University poultry farms, 10 samples were randomly collected from 500 birds. At Federal Polytechnic Ado Ekiti poultry farms, 10 samples were randomly collected from 700 birds. The bird’s selection was on the basis of clinical signs and symptoms such as difficulty in breathing, weight loss, drooping of wings and exercise intolerance. Swab samples were collected from each suspected/sick bird for mycological culture and molecular characterization of the isolates from each bird was carried out. The isolates were identified based on the color of the culture on Potato Dextrose Agar and microscopic examination. Molecular identification was done using 23S Ribosomal RNA Gene and Partial Sequence. Results: Six fungal strains that showed similar morphological and cultural characteristics of Aspergillus species were isolated. The isolates were coded ASP 1, ASP 2, ASP 3, ASP 4, ASP 5, and ASP 6. The identified organisms were; Aspergillus fumigatus qH 107 (ASP 1), Aspergillus fumigatus qH 107 (ASP 2), Aspergillus flavus M09 (ASP 3), Aspergillus flavus UOMS6 (ASP 4), Aspergillus fumigatus qH 107 (ASP 5), Aspergillus flavus qH 107 (ASP 6). Conclusion: It is evident that Aspergillus species were predominant in poultry farms selected in this study. Necessary precaution should be put in place to prevent the spread of aspergillosis. Poultry farmers are advised to avoid damp environments, moldy feeds, dry and dusty litters. Adequate ventilation should always be provided in poultry farms to prevent Aspergillosis.

scholarly journals Molecular Characterization of Chelonid Alphaherpesvirus 5 in a Black Turtle (Chelonia mydas) Fibropapilloma from Baja California Sur, Mexico

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 105
Author(s):  
Eduardo Reséndiz ◽  
Helena Fernández-Sanz ◽  
José Francisco Domínguez-Contreras ◽  
Amelly Hyldaí Ramos-Díaz ◽  
Agnese Mancini ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Molecular Analysis ◽  
Baja California ◽  
Clinical Signs ◽  
Chelonia Mydas ◽  
Baja California Sur ◽  
Epidermal Hyperplasia ◽  
Routine Monitoring ◽  
Hematological Values

During routine monitoring in Ojo de Liebre Lagoon, Mexico, a juvenile black turtle (Chelonia mydas) was captured, physically examined, measured, weighed, sampled, and tagged. The turtle showed no clinical signs suggestive of disease. Eleven months later, this turtle was recaptured in the same area, during which one lesion suggestive of fibropapilloma on the neck was identified and sampled for histopathology and molecular analysis. Histopathology revealed hyperkeratosis, epidermal hyperplasia, acanthosis, papillary differentiation and ballooning degeneration of epidermal cells, increased fibroblasts in the dermis, and angiogenesis, among other things. Hematological values were similar to those reported for clinically healthy black turtles and did not show notable changes between the first capture and the recapture; likewise, clinicopathological evaluation did not show structural or functional damage in the turtle’s systems. The chelonid alphaherpesvirus 5 (ChHV5) UL30 gene was amplified and sequenced for phylogeny; Bayesian reconstruction showed a high alignment with the genus Scutavirus of the Eastern Pacific group. This is one of the first reports of ChHV5 in a cutaneous fibropapilloma of a black turtle in the Baja California peninsula.

scholarly journals Detection and Molecular Characterization of Rotavirus and Picobirnavirus in Wild Avians from Amazon Forest

2020 ◽  
Author(s):  
José Wandilson Barboza Duarte Júnior ◽  
Elaine Hellen Nunes Chagas ◽  
Ana Carolina Silva Serra ◽  
Lizandra Caroline dos Santos Souto ◽  
Edvaldo Tavares da Penha Júnior ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Clinical Signs ◽  
Wild Birds ◽  
Interspecies Transmission ◽  
Amazon Forest ◽  
Rotavirus A ◽  
Exotic Birds ◽  
Veterinary Hospital ◽  
Nsp3 Gene

ABSTRACTThe present study reports the detection and molecular characterization of rotavirus A (RVA), rotavirus D (RVD), rotavirus F (RVF), rotavirus G (RVG) and picobirnavirus (PBV) in fecal specimens of wild and exotic birds (n = 23) from different cities of Pará state, which were hospitalized at Veterinary Hospital of the Federal University of Pará, Brazil, between January 2018 to June 2019. The animals exhibited different clinical signs, such as diarrhea, malnutrition, dehydration and fractures. The results showed 39.1% (9/23) of positivity for RVA by RT-qPCR. Among these, one sample (1/9) for the NSP3 gene of T2 genotype was characterized. About 88.9% (8/9) for the VP7 gene belonging to G1, equine-like G3 and G6 genotypes, and 55.5% (5/9) for the VP4 gene of P[2] genotype were obtained. In the current study, approximately 4.5% of the samples (1/23) revealed coinfection for the RVA, RVD and RVF groups. Furthermore, picobirnavirus (PBV) was detected in 1 of the 23 samples tested and was classified in the Genogroup I. The findings represent the first report of the circulation of RVA, RVD, RVF, RVG and PBV genotypes in wild birds in Brazil and suggest the possible interspecies transmission of RVs and PBVs.

scholarly journals Molecular characterization of lumpy skin disease virus (LSDV) emerged in Bangladesh reveals unique genetic features compared to contemporary field strains

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Shukes Chandra Badhy ◽  
Mohammad Golam Azam Chowdhury ◽  
Tirumala Bharani Kumar Settypalli ◽  
Giovanni Cattoli ◽  
Charles Euloge Lamien ◽  
...  
Keyword(s):  
Middle East ◽  
Molecular Characterization ◽  
Disease Virus ◽  
Skin Disease ◽  
Clinical Signs ◽  
Sequence Alignments ◽  
Multiple Sequence ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus

Abstract Background Lumpy skin disease (LSD) is a contagious viral disease of cattle caused by lumpy skin disease virus (LSDV). LSD has recently spread in Asia following outbreaks in the Middle East and Europe. The disease emerged in Bangladesh in July 2019 in the Chattogram district, then rapidly spread throughout the entire country. We investigated six LSD outbreaks in Bangladesh to record the clinical signs and collect samples for diagnostic confirmation. Furthermore, we performed the molecular characterization of Bangladesh isolates, analyzing the full RPO30 and GPCR genes and the partial EEV glycoprotein gene. Results Clinical observations revealed common LSD clinical signs in the affected cattle. PCR and real-time PCR, showed the presence of the LSDV genome in samples from all six districts. Phylogenetic analysis and detailed inspection of multiple sequence alignments revealed that Bangladesh isolates differ from common LSDV field isolates encountered in Africa, the Middle East, and Europe, as well as newly emerged LSDV variants in Russia and China. Instead, they were closely related to LSDV KSGP-0240, LSDV NI2490, and LSDV Kenya. Conclusions These results show the importance of continuous monitoring and characterization of circulating strains and the need to continually refine the strategies for differentiating vaccine strains from field viruses.

scholarly journals Molecular Characterization of a Hamster Viscerotropic Strain of Yellow Fever Virus

2003 ◽  
Vol 77 (2) ◽  
pp. 1462-1468 ◽  
Author(s):  
Monica A. McArthur ◽  
Miguel T. Suderman ◽  
John-Paul Mutebi ◽  
Shu-Yuan Xiao ◽  
Alan D. T. Barrett
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Yellow Fever ◽  
Yellow Fever Virus ◽  
Clinical Signs ◽  
Serial Passage ◽  
Severe Illness ◽  
Amino Acid Substitutions ◽  
E Protein

ABSTRACT A hamster viscerotropic strain of yellow fever (YF) virus has been derived after serial passage of strain Asibi through hamsters. The parental Asibi/hamster p0 virus causes a mild and transient viremia in hamsters with no outward, clinical signs of illness. In contrast, the viscerotropic Asibi/hamster p7 virus causes a robust viremia, severe illness, and death in subadult hamsters. The genome of the hamster viscerotropic Asibi/hamster p7 virus has been sequenced and compared with the parental nonviscerotropic Asibi/hamster p0 virus identifying 14 nucleotide changes encoding only seven amino acid substitutions. The majority of these substitutions (five of seven) fall within the envelope (E) protein at positions Q27H, D28G, D155A, K323R, and K331R. These results support an important role for the E protein in determining YF virus viscerotropism.

Sign in / Sign up

Export Citation Format

Share Document