Validation, Implementation, and Clinical Utility of Whole Genome Sequence-Based Bacterial Identification in the Clinical Microbiology Laboratory

2021 ◽  
Author(s):  
Travis K. Price ◽  
Susan Realegeno ◽  
Ruel Mirasol ◽  
Allison Tsan ◽  
Sukantha Chandrasekaran ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Clinical Utility ◽  
Clinical Microbiology ◽  
Whole Genome Sequence ◽  
Clinical Microbiology Laboratory ◽  
Whole Genome ◽  
Bacterial Identification ◽  
Microbiology Laboratory

scholarly journals Molecular Assay Validation Using Genomic Sequence Databases

2016 ◽  
Vol 54 (12) ◽  
pp. 2854-2856 ◽  
Author(s):  
John P. Dekker
Keyword(s):  
Clinical Microbiology ◽  
Genomic Sequence ◽  
Sequence Data ◽  
Whole Genome Sequence ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Molecular Assay ◽  
Pcr Assay ◽  
Content Type ◽  
Sequence Databases

Whole-genome sequence databases offer newin silicoapproaches for designing and validating PCR assays in the clinical microbiology laboratory. An article in this issue of theJournal of Clinical Microbiology(M. J. Jansen van Rensburg, C. Swift, A. J. Cody, C. Jenkins, and M. C. J. Maiden, J Clin Microbiol, 54:2882–2890, 2016,http://dx.doi.org/10.1128/JCM.01522-16) demonstrates the use of publicly available genomic sequence data to evaluate a PCR assay for distinguishingCampylobacterspecies.

scholarly journals Serum bactericidal test.

1988 ◽  
Vol 1 (1) ◽  
pp. 19-26 ◽  
Author(s):  
C W Stratton
Keyword(s):  
Clinical Trials ◽  
Clinical Utility ◽  
Clinical Microbiology ◽  
Additional Data ◽  
In Vitro Tests ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
New Information ◽  
Performance Results

The serum bactericidal test represents one of the few in vitro tests performed in the clinical microbiology laboratory that combines the interaction of the pathogen, the antimicrobial agent, and the patient. Although the use of such a test antedates the antimicrobial era, its performance, results, and interpretation have been subject to question and controversy. Much of the confusion concerning the serum bactericidal test can be avoided by an understanding of the various factors which influence bactericidal testing. In addition, the methodologic aspects of the serum bactericidal test have recently been addressed and should place this test on firmer ground. New information on the clinical utility of this test is becoming available; additional data are needed to establish more clearly the usefulness of the serum bactericidal test in specific infections. Such clinical trials from multiple centers will enable firmer recommendations for the future use of the serum bactericidal test.

scholarly journals Lowering the Barriers to Routine Whole-Genome Sequencing of Bacteria in the Clinical Microbiology Laboratory

2018 ◽  
Vol 56 (9) ◽  
Author(s):  
Daniel D. Rhoads
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Clinical Microbiology ◽  
Sequence Data ◽  
Whole Genome Sequence ◽  
Clinical Microbiology Laboratory ◽  
Whole Genome ◽  
Content Type ◽  
Antibacterial Susceptibility ◽  
Susceptibility And Resistance

ABSTRACT Whole-genome sequencing of bacterial isolates is increasingly being used to predict antibacterial susceptibility and resistance. Mason and coauthors describe the phenotypic susceptibility interpretations of more than 1,300 Staphylococcus aureus isolates tested against a dozen antistaphylococcal agents, and they compared these findings to susceptibility predictions made by analyzing whole-genome sequence data (J Clin Microbiol 56:e01815-17, 2018, https://doi.org/10.1128/JCM.01815-17). The genotype-phenotype susceptibility interpretations correlated in 96.3% (2,720/2,825) of resistant findings and 98.8% (11,504/11,639) of susceptible findings. This work by Mason and colleagues is helping to lower the barriers to using whole-genome sequencing of S. aureus in clinical microbiology practice.

MALDI-TOF mass spectrometry tools for bacterial identification in clinical microbiology laboratory

2011 ◽  
Vol 44 (1) ◽  
pp. 104-109 ◽  
Author(s):  
Etienne Carbonnelle ◽  
Cécile Mesquita ◽  
Emmanuelle Bille ◽  
Nesrine Day ◽  
Brunhilde Dauphin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Clinical Microbiology ◽  
Clinical Microbiology Laboratory ◽  
Bacterial Identification ◽  
Microbiology Laboratory ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof
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