scholarly journals Selective isolation of mouse glial nuclei optimized for reliable downstream omics analyses

2022 ◽  
pp. 109480
Author(s):  
Miguel A. Pena-Ortiz ◽  
Sarfraz Shafiq ◽  
Megan E Rowland ◽  
Nathalie G Bérubé
Keyword(s):  
Selective Isolation

A method for selective separation of scandium (III) from yttrium and a number of rare-earth elements for its subsequent determination

2018 ◽  
Vol 84 (11) ◽  
pp. 23-27
Author(s):  
M. I. Degtev ◽  
A. A. Yuminova ◽  
A. S. Maksimov ◽  
A. P. Medvedev
Keyword(s):  
Rare Earth ◽  
Aqueous Phase ◽  
Selective Isolation ◽  
Optimum Ratio ◽  
Extraction Condition ◽  
Sulfosalicylic Acid ◽  
Salting Out ◽  
Subsequent Determination ◽  
Toxic Organic Solvents ◽  
Metal Ions Extraction

The possibility of using an aqueous stratified system of antipyrine — sulfosalicylic acid — water for the selective isolation of scandium macro- and microquantities for subsequent determination is studied. The proposed extraction system eliminates the usage of toxic organic solvents. The organic phase with a volume of 1.2 to 2.0 ml, resulting from delamination of the aqueous phase containing antipyrine and sulfosalicylic acid is analysed to assess the possibility of using such systems for metal ions extraction. Condition necessary for the formation of such a phase were specified: the ratio of the initial components, their concentration, presence of inorganic salting out agents. The optimum ratio of antipyrine to sulfosalicylic acid is 2:1 at concentrations of 0.6 and 0.3 mol/liter in a volume of the aqueous phase of 10 ml. The obtained phase which consists of antipyrinium sulfosalicylate, free antipyrine and water, quantitatively extracts macro- and microquantities of scandium at pH = 1.54. Macro- and microquantities of yttrium, terbium, lanthanum, ytterbium and gadolinium are not extracted under the aforementioned conditions thus providing selective isolation of scandium from the bases containing yttrium, ytterbium, terbium, lanthanum, and gadolinium.

SELECTIVE ISOLATION OF HUMAN X-BEARING SPERM BY DIFFERENTIAL VELOCITY SEDIMENTATION IN PERCOLL DENSITY GRADIENTS

1984 ◽  
Vol 5 (2) ◽  
pp. 187-194 ◽  
Author(s):  
SATORU KANEKO ◽  
SHIGERU OSHIO ◽  
TOSHIFUMI KOBAYASHI ◽  
HIDEO MOHRI ◽  
RIHACHI IIZUKA
Keyword(s):  
Velocity Sedimentation ◽  
Density Gradients ◽  
Selective Isolation

Efficient and selective isolation of local anaesthetics from biological fluids using C18 silica gel

1984 ◽  
Vol 286 ◽  
pp. 223-227 ◽  
Author(s):  
Ladislav Šoltés ◽  
Dušan Berek ◽  
Milan Štefek
Keyword(s):  
Silica Gel ◽  
Biological Fluids ◽  
Local Anaesthetics ◽  
Selective Isolation

Selective isolation of nitrogen bases from petroleum

1983 ◽  
Vol 55 (11) ◽  
pp. 1685-1688 ◽  
Author(s):  
Jean Marie. Schmitter ◽  
Ioannis. Ignatiadis ◽  
Patrick. Arpino ◽  
Georges. Guiochon
Keyword(s):  
Selective Isolation ◽  
Nitrogen Bases

scholarly journals Immunomagnetic Separation and Coagglutination of Vibrio parahaemolyticus with Anti-Flagellar Protein Monoclonal Antibody

2008 ◽  
Vol 15 (10) ◽  
pp. 1541-1546 ◽  
Author(s):  
S. Datta ◽  
M. E. Janes ◽  
J. G. Simonson
Keyword(s):  
Staphylococcus Aureus ◽  
Monoclonal Antibody ◽  
Cell Suspension ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Immunomagnetic Separation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Selective Isolation ◽  
Flagellar Protein ◽  
Phosphate Buffered Saline

ABSTRACT Mice were immunized by injection of Vibrio parahaemolyticus ATCC 17802 polar flagellin in order to produce monoclonal antibodies (mAbs). mAbs were analyzed by anti-H enzyme-linked immunosorbent assay using V. parahaemolyticus polar flagellar cores. The mAb exhibiting the highest anti-H titer was coated onto Cowan I Staphylococcus aureus cells at a concentration of 75 μg/ml cell suspension and used for slide coagglutination. Of 41 isolates identified genetically as V. parahaemolyticus, 100% coagglutinated with the anti-H mAb within 30 s, and the mAb did not react with 30 isolates identified as Vibrio vulnificus. A strong coagglutination reaction with V. parahaemolyticus ATCC 17802 was still observed when the S. aureus cells were armed with as little as 15 μg of mAb/ml S. aureus cell suspension. At this concentration, the mAb cross-reacted with three other Vibrio species, suggesting that they share an identical H antigen or antigens. The anti-H mAb was then used to optimize an immunomagnetic separation protocol which exhibited from 35% to about 45% binding of 102 to 103 V. parahaemolyticus cells in phosphate-buffered saline. The mAb would be useful for the rapid and selective isolation, concentration, and detection of V. parahaemolyticus cells from environmental sources.

In silico analysis of accurate proteomics, complemented by selective isolation of peptides

2011 ◽  
Vol 74 (10) ◽  
pp. 2071-2082 ◽  
Author(s):  
Yasset Perez-Riverol ◽  
Aniel Sánchez ◽  
Yassel Ramos ◽  
Alex Schmidt ◽  
Markus Müller ◽  
...  
Keyword(s):  
In Silico ◽  
In Silico Analysis ◽  
Selective Isolation ◽  
Silico Analysis

Graphene Oxide–Rare Earth Metal–Organic Framework Composites for the Selective Isolation of Hemoglobin

2014 ◽  
Vol 6 (13) ◽  
pp. 10196-10204 ◽  
Author(s):  
Jia-Wei Liu ◽  
Yue Zhang ◽  
Xu-Wei Chen ◽  
Jian-Hua Wang
Keyword(s):  
Graphene Oxide ◽  
Rare Earth ◽  
Rare Earth Metal ◽  
Metal Organic Framework ◽  
Earth Metal ◽  
Selective Isolation ◽  
Metal Organic ◽  
Organic Framework

A method for terminus proteomics: Selective isolation and labeling of N-terminal peptide from protein through transamination reaction

2009 ◽  
Vol 19 (23) ◽  
pp. 6544-6547 ◽  
Author(s):  
Kazuhiro Sonomura ◽  
Hiroki Kuyama ◽  
Ei-ichi Matsuo ◽  
Susumu Tsunasawa ◽  
Osamu Nishimura
Keyword(s):  
Selective Isolation ◽  
Transamination Reaction
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