Cartilage repair of the ankle: first results of T2 mapping at 7.0 T after microfracture and matrix associated autologous cartilage transplantation

Objective. To evaluate minimum biosecurity parameters (MBP) for arthroscopic matrix-encapsulated autologous chondrocyte implantation (AMECI) based on patients’ clinical outcomes, magnetic resonance imaging (MRI) T2-mapping, Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) score, and International Cartilage Repair Society (ICRS) second-look arthroscopic evaluation, laying the basis for a future multicenter study. Design. Pilot clinical study. We analyzed the logistics to perform AMECI to treat focal chondral lesions in different hospitals following strict biosecurity parameters related to tissue and construct transportation, chondrocyte isolation, and cell expansion. Patient progress was analyzed with patient-reported outcome measures, MRI T2-mapping, MOCART, and ICRS arthroscopic second-look evaluation. Results. Thirty-five lesions in 30 patients treated in 7 different hospitals were evaluated. Cell viability before implantation was >90%. Cell viability in construct remnants was 87% ± 11% at 24 hours, 75% ± 17.1% at 48 hours, and 60% ± 8% at 72 hours after implantation. Mean final follow-up was 37 months (12-72 months). Patients showed statistically significant improvement in all clinical scores and MOCART evaluations. MRI T2-mapping evaluation showed significant decrease in relaxation time from 61.2 ± 14.3 to 42.9 ± 7.2 ms ( P < 0.05). Arthroscopic second-look evaluation showed grade II “near normal” tissue in 83% of patients. Two treatment failures were documented. Conclusions. It was feasible to perform AMECI in 7 different institutions in a large metropolitan area following our biosecurity measures without any implant-related complication. Treated patients showed improvement in clinical, MRI T2-mapping, and MOCART scores, as well as a low failure rate and a favorable ICRS arthroscopic evaluation at a mid-term follow-up. Level of Evidence. 2b.

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Evaluation and comparison of cartilage repair tissue of the patella and medial femoral condyle by using morphological MRI and biochemical zonal T2 mapping

European Radiology ◽

10.1007/s00330-008-1249-6 ◽

2008 ◽

Vol 19 (5) ◽

pp. 1253-1262 ◽

Cited By ~ 41

Author(s):

Goetz H. Welsch ◽

Tallal C. Mamisch ◽

Sebastian Quirbach ◽

Lukas Zak ◽

Stefan Marlovits ◽

...

Keyword(s):

Cartilage Repair ◽

T2 Mapping ◽

Femoral Condyle ◽

Medial Femoral Condyle ◽

Repair Tissue

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T2 mapping: an efficient MR quantitative technique to evaluate spontaneous cartilage repair in rat patella11This work was supported by grants from Projet Hospitalier de Recherche Clinique (1998), the Contrat de Projet de Recherche Clinique (2000), ‘Fondation pour la Recherche Médicale’, ‘Région Lorraine’ and ‘Groupement de Recherches CNRS 2237’.

Osteoarthritis and Cartilage ◽

10.1016/j.joca.2003.10.010 ◽

2004 ◽

Vol 12 (3) ◽

pp. 191-200 ◽

Cited By ~ 33

Author(s):

A Watrin-Pinzano ◽

J.-P Ruaud ◽

Y Cheli ◽

P Gonord ◽

L Grossin ◽

...

Keyword(s):

Cartilage Repair ◽

T2 Mapping ◽

Recherche Clinique ◽

Quantitative Technique ◽

Recherche Médicale

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Matrix-induced autologous chondrocyte implantation grafting in osteochondral lesions of the talus: Evaluation of cartilage repair using T2 mapping

Journal of Orthopaedics ◽

10.1016/j.jor.2019.04.002 ◽

2019 ◽

Vol 16 (6) ◽

pp. 500-503 ◽

Cited By ~ 1

Author(s):

P. Correa Bellido ◽

J. Wadhwani ◽

E. Gil Monzo

Keyword(s):

Cartilage Repair ◽

Autologous Chondrocyte Implantation ◽

T2 Mapping ◽

Osteochondral Lesions ◽

Chondrocyte Implantation ◽

Autologous Chondrocyte

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Cartilage Repair Surgery: Outcome Evaluation by Using Noninvasive Cartilage Biomarkers Based on Quantitative MRI Techniques?

BioMed Research International ◽

10.1155/2014/840170 ◽

2014 ◽

Vol 2014 ◽

pp. 1-17 ◽

Cited By ~ 26

Author(s):

Pia M. Jungmann ◽

Thomas Baum ◽

Jan S. Bauer ◽

Dimitrios C. Karampinos ◽

Benjamin Erdle ◽

...

Keyword(s):

Mr Imaging ◽

Cartilage Repair ◽

T2 Mapping ◽

Outcome Evaluation ◽

Quantitative Mri ◽

Surgery Outcome ◽

Quantitative Mr Imaging ◽

Quantitative Mr ◽

Imaging Characteristics ◽

Magnetic Resonance Imaging Mri

Background. New quantitative magnetic resonance imaging (MRI) techniques are increasingly applied as outcome measures after cartilage repair.Objective. To review the current literature on the use of quantitative MRI biomarkers for evaluation of cartilage repair at the knee and ankle.Methods. Using PubMed literature research, studies on biochemical, quantitative MR imaging of cartilage repair were identified and reviewed.Results. Quantitative MR biomarkers detect early degeneration of articular cartilage, mainly represented by an increasing water content, collagen disruption, and proteoglycan loss. Recently, feasibility of biochemical MR imaging of cartilage repair tissue and surrounding cartilage was demonstrated. Ultrastructural properties of the tissue after different repair procedures resulted in differences in imaging characteristics. T2 mapping, T1rho mapping, delayed gadolinium-enhanced MRI of cartilage (dGEMRIC), and diffusion weighted imaging (DWI) are applicable on most clinical 1.5 T and 3 T MR scanners. Currently, a standard of reference is difficult to define and knowledge is limited concerning correlation of clinical and MR findings. The lack of histological correlations complicates the identification of the exact tissue composition.Conclusions. A multimodal approach combining several quantitative MRI techniques in addition to morphological and clinical evaluation might be promising. Further investigations are required to demonstrate the potential for outcome evaluation after cartilage repair.

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Clinical Study of Autologous Cartilage Transplantation Based on Nano-Hydroxyapatite in the Treatment of Talar Osteochondral Injury

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2021.18634 ◽

2021 ◽

Vol 21 (2) ◽

pp. 1250-1258

Author(s):

Weijun Wang ◽

Xiqiang Wang ◽

Yongmei Wang ◽

Changgui Tong

Keyword(s):

New Zealand ◽

Subchondral Bone ◽

Cartilage Damage ◽

Clinical Treatment ◽

Bone Lesions ◽

Osteochondral Defects ◽

Cartilage Transplantation ◽

Bone Injury ◽

Autologous Cartilage Transplantation

Talus osteochondral damage is one of the common symptoms of chronic ankle pain in people’s lives. The cartilage regeneration and self-repair ability are extremely limited, the joint cartilage lesions are often accompanied by the lesions of the subchondral bone, and the subchondral bone lesions can affect the metabolism of the cartilage above it, which brings certain difficulties to clinical treatment. Traditional methods of treating cartilage damage include microfractures and drilling. Due to large trauma, inconsistent clinical efficacy reports, poor tissue repair results, and limited donor sources, etc., the application of traditional treatment methods in the clinic has been largely limited. Therefore, finding an ideal treatment method for bone injury has been a hot spot in clinical research in orthopedics. Studies have shown that autologous cartilage transplantation via nano-hydroxyapatite has become a new treatment model, providing new ideas for clinical treatment of talar osteochondral damage. Nano-hydroxyapatite and its composites have good histocompatibility, biological activity, and bone conductivity. They are an ideal bone defect repair material, and have been initially applied in clinical practice. The preparation of nano-hydroxyapatite, its biological characteristics and the repairing effect on the composite defect of osteochondral bone were studied experimentally, and its feasibility for repairing osteochondral damage was discussed. In this paper, the unique structure and properties of natural cartilage layers are studied. In combination with bionics theory and methods, nano-hydroxyapatite micro-particle composite samples are prepared by the gel method, and the bone-forming properties of nano-composites are measured by in vitro drug release experiments. To establish a model of infectious bone injury in New Zealand white rabbits, and nano-hydroxyapatite composites were implanted into local lesions of New Zealand white rabbit models by autologous cartilage transplantation, and evaluated by imaging, blood biochemistry, histology, infection control and bone repair. The experimental results show that using the unique physical and chemical and biological properties of nano-hydroxyapatite materials. It is innovatively introduced into the treatment of talar osteochondral defects caused by open fractures. It has been proven in vitro and in vivo experiments that nano-hydroxyapatite materials can be used. As an ideal tissue engineering scaffold for the treatment of talar osteochondral defects, this provides a new way to solve clinical orthopedic problems using new nanomaterials.

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