scholarly journals Validated HPLC method for identification and quantification of p -hydroxy benzoic acid and agnuside in Vitex negundo and Vitex trifolia

2013 ◽  
Vol 3 (6) ◽  
pp. 500-508 ◽  
Author(s):  
Sonal Shah ◽  
Tushar Dhanani ◽  
Satyanshu Kumar
Keyword(s):  
Benzoic Acid ◽  
Hplc Method ◽  
Vitex Negundo ◽  
Identification And Quantification

scholarly journals A validated high performance liquid chromatography method for determination of three bioactive compounds p-hydroxy benzoic acid, negundoside and agnuside in Vitex species

2015 ◽  
Vol 34 (2) ◽  
pp. 321 ◽  
Author(s):  
Tushar Dhanani ◽  
Sonal Shah ◽  
Satyanshu Kumar
Keyword(s):  
Benzoic Acid ◽  
High Performance ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Hplc Method ◽  
Vitex Negundo ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Vitex Species

A validated rapid and simple isocratic HPLC-PDA method was developed for identification and quantification of p- hydroxy benzoic acid and two iridoids negundoside and agnuside in the extracts of two Vitex species, Vitex negundo and Vitex trifolia. The separation of the three compounds was achieved on a RP-18 (250 mm X 4 mm, 5µm) column at 25o C using acetonitrile (15%) and 0.05% trifluoroacetic acid (TFA) in water (85%). Limit of detection (LOD) were 1, 2.5 and 2.5 μg/ml for p- hydroxy benzoic acid, negundoside and agnuside respectively. Similarly, limit of quantification (LOQ) were 2.5, 5 and 5 μg/ml for p- hydroxy benzoic acid, negundoside and agnuside respectively. Good linearity (r2 > 0.999) was observed for all the three compounds in wide concentration range. Using the developed HPLC method, the three compounds were identified and quantified in leaves and bark extracts of Vitex negundo and Vitex trifolia. The novelty of the developed HPLC method is that it does not require complex sample processing such as use of solid phase extraction as well as use of buffer in mobile phase. This is the first report of a validated HPLC method for the simultaneous determination of p- hydroxy benzoic acid, negundoside and agnuside in Vitex species.

scholarly journals Determination of Preservatives in Fruit Juice Products Available in Bangladesh by a Validated RP HPLC Method

2019 ◽  
Vol 18 (2) ◽  
pp. 195-208
Author(s):  
Md Samiul Islam ◽  
Nisat Zahan ◽  
Md Shahadat Hossain ◽  
Abu Shara Shamsur Rouf
Keyword(s):  
Benzoic Acid ◽  
Fruit Juice ◽  
Sorbic Acid ◽  
Hplc Method ◽  
Fruit Juices ◽  
Expert Committee ◽  
Methyl Paraben ◽  
Propyl Paraben ◽  
Rp Hplc ◽  
Acid Methyl

The aim of this study was to investigate whether fruit juices available in markets of Bangladesh contain any preservative. A specific RP-HPLC method was developed, validated and applied to identify and quantify preservatives including benzoic acid, sorbic acid, methyl paraben and propyl paraben simultaneously in 50 different products. These additives were separated by C18 column in mobile phase composed of methanol and acetate buffer (pH 4.4) in the ratio of 50:50 with a flow rate of 0.7 mL/min, and detected at 254 nm. Linearities for benzoic acid, sorbic acid, methyl paraben and propyl paraben were determined in the range of 20-170 ppm (r2 0.997), 12-42 ppm (r2 0.994), 10-60 ppm (r2 0.993) and 10-60 ppm (r2 0.992) respectively. Limit of detection (LOD) and limit of quantification (LOQ) were 5.46 ppm and 16.5 ppm for benzoic acid while for sorbic acid they were 1.08 ppm and 3.30 ppm, respectively. Benzoic acid was detected in a range of 96.1 to 441 ppm in 9 fruit juices while in 7 fruit juices sorbic acid was found in a range of 105 - 444 ppm. The values were within the maximum allowable ranges for fruit juice (1000 ppm for both benzoic acid and sorbic acid) as suggested by the Joint FAO/WHO Expert Committee on Food Additives (JECFA). None of the juice product was found to contain methyl paraben or propyl paraben Dhaka Univ. J. Pharm. Sci. 18(2): 195-208, 2019 (December)

scholarly journals Identification and Quantification of Andrographolide from Andrographis paniculata (Burm. f.) Wall. ex Nees by RP-HPLC Method and Standardization of its Market Preparations

2015 ◽  
Vol 14 (1) ◽  
pp. 71-78 ◽  
Author(s):  
BK Sajeeb ◽  
Uttom Kumar ◽  
Shimul Halder ◽  
Sitesh C Bachar
Keyword(s):  
Quantitative Estimation ◽  
Reversed Phase ◽  
Hplc Method ◽  
Andrographis Paniculata ◽  
Plant Sample ◽  
Rp Hplc ◽  
Ft Ir ◽  
Therapeutic Activities ◽  
Identification And Quantification

Andrographis paniculata (Burm. f.) Wall. ex Nees, commonly known as Kalmegh, is widely used as antimalarial drug in herbal and traditional systems. Andrographolide is the major triterpenoid present in the plant and responsible for its therapeutic activities. The identification and quantification of andrographolide were ascertained by various spectrophotometric and chromatographic analyses. The plant sample was extracted with methanol. The amorphous residue obtained from extraction was analyzed for identification of andrographolide by chemical method, TLC, UV-Vis, FT-IR and LCMS/MS analyses. The quantitative estimation of andrographolide content in plant sample was carried out by simple reversed phase HPLC method with C18 column using a mixture of water and methanol (35:65) as mobile phase at a flow rate of 0.7 mL/min, and the estimated concentration level of andrographolide was found as 38.36±0.42 ?g/mL in the solution of amorphous residue (50 ?g/mL). After quantitative determination of andrographolide, standardization of its market preparations was accomplished using the same RP-HPLC method. The estimated % potencies of andrographolide compared to reference standard in six market preparations were found to be 97.56, 98.37, 98.21, 95.60, 98.91 and 96.40.Dhaka Univ. J. Pharm. Sci. 14(1): 71-78, 2015 (June)

scholarly journals A Simple and Effective Isocratic HPLC Method for Fast Identification and Quantification of Surfactin

2015 ◽  
Vol 44 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Muhammad Qadri Effendy Mubarak ◽  
Abdul Rahman Hassan ◽  
Aidil Abdul Hamid ◽  
Sahaid Khalil ◽  
Mohd. Hafez Mohd. Isa
Keyword(s):  
Hplc Method ◽  
Fast Identification ◽  
Isocratic Hplc ◽  
Identification And Quantification

A Rapid and Accurate 1HNMR Method for the Identification and Quantification of Major Constituents in Qishen Yiqi Dripping Pills

2020 ◽  
Author(s):  
Qingling Xie ◽  
Limin Gong ◽  
Feibing Huang ◽  
Mengru Cao ◽  
Yongbei Liu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Chemical Shifts ◽  
Salvia Miltiorrhiza ◽  
Proton Nmr ◽  
Herbal Medicines ◽  
Panax Notoginseng ◽  
Hplc Method ◽  
Coupling Constants ◽  
Identification And Quantification

Abstract Background Qishen Yiqi dripping pills (QSYQ), composed of four herbal medicines—Salvia miltiorrhiza, Astragalus membranaceus, Panax notoginseng, and Dalbergiaodorifera—are widely used to treat ischemic cerebrovascular and hemorrhagic cerebrovascular conditions. Objective In this study, a rapid and accurate proton NMR (1HNMR) spectroscopy method was established to control the quality of QSYQ and ensure their clinical efficacy. Method Firstly, different types of metabolites were identified based on the proton signal peaks of chemical shifts, coupling constants, and related information provided through two-dimensional NMR spectroscopy. Secondly, a quantitative 1HNMR method was established for the simultaneous determination of major constituents in QSYQ samples. In addition, an HPLC method was performed to verify the results obtained by the quantitative proton NMR (qHNMR)  method. Results In the present study, 26 metabolites were identified in the 1HNMR spectra of QSYQ. In addition, a rapid and accruate qHNMR method was established for the simultaneous determination of protocatechualdehyde, rosmarinic acid, danshensu, calycosin-7-O-β-D-glucoside, and ononin in ten batches of QSYQ samples for the first time. Moreover, the proposed qHNMR method and HPLC method were compared using Bland-Altman and plots Passing-Bablok regression, indicating no significant differences and a strong correlation between the two analytical methods. Conclusions This method is an important tool for the identification and quantification of major constituents in QSYQ. Highlights Compared with traditional HPLC, the established qHNMR method has the advantages of simple sample preparation, short analysis time, and non-destructive analysis.

Sign in / Sign up

Export Citation Format

Share Document