scholarly journals A Simple and Effective Isocratic HPLC Method for Fast Identification and Quantification of Surfactin

2015 ◽  
Vol 44 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Muhammad Qadri Effendy Mubarak ◽  
Abdul Rahman Hassan ◽  
Aidil Abdul Hamid ◽  
Sahaid Khalil ◽  
Mohd. Hafez Mohd. Isa
Keyword(s):  
Hplc Method ◽  
Fast Identification ◽  
Isocratic Hplc ◽  
Identification And Quantification

Fast and isocratic HPLC-method for steviol glycosides analysis from Stevia rebaudiana leaves

2012 ◽  
Vol 7 (2) ◽  
pp. 147-154 ◽  
Author(s):  
Dominik Bergs ◽  
Bernhard Burghoff ◽  
Matthias Joehnck ◽  
Georg Martin ◽  
Gerhard Schembecker
Keyword(s):  
Stevia Rebaudiana ◽  
Hplc Method ◽  
Steviol Glycosides ◽  
Isocratic Hplc

Simultaneous determination of four vasoactive phytochemicals in different pharmaceutical preparations by a simple HPLC-DAD method

2016 ◽  
Vol 8 (8) ◽  
pp. 1858-1866 ◽  
Author(s):  
Fawzia Ibrahim ◽  
Mohie Khaled Sharaf El-Din ◽  
Rania Nabih El-Shaheny ◽  
Asmaa Kamal El-Deen ◽  
Kuniyoshi Shimizu
Keyword(s):  
Ascorbic Acid ◽  
Simultaneous Determination ◽  
Pharmaceutical Preparations ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Isocratic Hplc

A new, simple isocratic HPLC method was developed and validated for the simultaneous estimation of four vasoactive phytochemicals: ascorbic acid (ASC), rutin (RUT), hesperidin (HSP), and diosmin (DSM) in different pharmaceutical preparations.

scholarly journals Application and Validation of Simple Isocratic HPLC-UV-DAD Method with Dual Wavelength Detection for Ivabradine Determination and Its Application in the Study of Stress Degradation

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Joanna Nowakowska ◽  
Piotr Pikul ◽  
Marcin Marszałł ◽  
Krzesimir Ciura
Keyword(s):  
Degradation Products ◽  
Hplc Method ◽  
Simple Method ◽  
Degradation Study ◽  
Modern Drug ◽  
Stress Degradation ◽  
Isocratic Hplc ◽  
Cardiac Energy ◽  
Wavelength Detection

Ivabradine is a modern drug that selectively lowers the heart rate, improves cardiac energy balance, and reduces heart’s demands for oxygen and energy. Due to the chemical nature of ivabradine, which absorbs light at 207 nm and 286 nm, its detection was performed at two wavelengths. A Knauer C8 column was used to develop the RP-HPLC method for determination of ivabradine. The proposed method was linear from 5 to 100 µg/ml (r>0.999) for both wavelengths and limits of detection (LOD) and limits of quantification (LOQ) were 0.33 and 1.09 µg/ml for 207 nm and 1.19 and 3.97 µg/ml for 286 nm, respectively. After validation, the investigated method was applied to a stress degradation study. Numerous degradation products were formed from ivabradine solutions through alkaline and acid hydrolysis, oxidation, and photolysis. The largest numbers of degradation products were found in the sample exposed to 24 h radiation and alkaline hydrolysis (eight and six products, resp.). Finally, the simple method using HPLC-UV-DAD was developed and validated. Its usefulness for the monitoring of possible degradation products was demonstrated.

scholarly journals Identification and Quantification of Andrographolide from Andrographis paniculata (Burm. f.) Wall. ex Nees by RP-HPLC Method and Standardization of its Market Preparations

2015 ◽  
Vol 14 (1) ◽  
pp. 71-78 ◽  
Author(s):  
BK Sajeeb ◽  
Uttom Kumar ◽  
Shimul Halder ◽  
Sitesh C Bachar
Keyword(s):  
Quantitative Estimation ◽  
Reversed Phase ◽  
Hplc Method ◽  
Andrographis Paniculata ◽  
Plant Sample ◽  
Rp Hplc ◽  
Ft Ir ◽  
Therapeutic Activities ◽  
Identification And Quantification

Andrographis paniculata (Burm. f.) Wall. ex Nees, commonly known as Kalmegh, is widely used as antimalarial drug in herbal and traditional systems. Andrographolide is the major triterpenoid present in the plant and responsible for its therapeutic activities. The identification and quantification of andrographolide were ascertained by various spectrophotometric and chromatographic analyses. The plant sample was extracted with methanol. The amorphous residue obtained from extraction was analyzed for identification of andrographolide by chemical method, TLC, UV-Vis, FT-IR and LCMS/MS analyses. The quantitative estimation of andrographolide content in plant sample was carried out by simple reversed phase HPLC method with C18 column using a mixture of water and methanol (35:65) as mobile phase at a flow rate of 0.7 mL/min, and the estimated concentration level of andrographolide was found as 38.36±0.42 ?g/mL in the solution of amorphous residue (50 ?g/mL). After quantitative determination of andrographolide, standardization of its market preparations was accomplished using the same RP-HPLC method. The estimated % potencies of andrographolide compared to reference standard in six market preparations were found to be 97.56, 98.37, 98.21, 95.60, 98.91 and 96.40.Dhaka Univ. J. Pharm. Sci. 14(1): 71-78, 2015 (June)

scholarly journals Validation of an isocratic HPLC method to detect 2-fluoro-β-alanine for the analysis of dihydropyrimidine dehydrogenase activity

2010 ◽  
Vol 878 (21) ◽  
pp. 1889-1892
Author(s):  
Kinta M. Serve ◽  
Jennifer L. Darnell ◽  
Jody K. Takemoto ◽  
Neal M. Davies ◽  
Margaret E. Black
Keyword(s):  
Dehydrogenase Activity ◽  
Dihydropyrimidine Dehydrogenase ◽  
Hplc Method ◽  
Isocratic Hplc

scholarly journals A Simple Isocratic HPLC Method for the Quantitation of 17 Cannabinoids

2021 ◽  
Author(s):  
Peter Galettis ◽  
Michelle Williams ◽  
Rebecca Gordon ◽  
Jennifer H. Martin
Keyword(s):  
Hplc Method ◽  
Isocratic Hplc

A Rapid and Accurate 1HNMR Method for the Identification and Quantification of Major Constituents in Qishen Yiqi Dripping Pills

2020 ◽  
Author(s):  
Qingling Xie ◽  
Limin Gong ◽  
Feibing Huang ◽  
Mengru Cao ◽  
Yongbei Liu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Chemical Shifts ◽  
Salvia Miltiorrhiza ◽  
Proton Nmr ◽  
Herbal Medicines ◽  
Panax Notoginseng ◽  
Hplc Method ◽  
Coupling Constants ◽  
Identification And Quantification

Abstract Background Qishen Yiqi dripping pills (QSYQ), composed of four herbal medicines—Salvia miltiorrhiza, Astragalus membranaceus, Panax notoginseng, and Dalbergiaodorifera—are widely used to treat ischemic cerebrovascular and hemorrhagic cerebrovascular conditions. Objective In this study, a rapid and accurate proton NMR (1HNMR) spectroscopy method was established to control the quality of QSYQ and ensure their clinical efficacy. Method Firstly, different types of metabolites were identified based on the proton signal peaks of chemical shifts, coupling constants, and related information provided through two-dimensional NMR spectroscopy. Secondly, a quantitative 1HNMR method was established for the simultaneous determination of major constituents in QSYQ samples. In addition, an HPLC method was performed to verify the results obtained by the quantitative proton NMR (qHNMR)  method. Results In the present study, 26 metabolites were identified in the 1HNMR spectra of QSYQ. In addition, a rapid and accruate qHNMR method was established for the simultaneous determination of protocatechualdehyde, rosmarinic acid, danshensu, calycosin-7-O-β-D-glucoside, and ononin in ten batches of QSYQ samples for the first time. Moreover, the proposed qHNMR method and HPLC method were compared using Bland-Altman and plots Passing-Bablok regression, indicating no significant differences and a strong correlation between the two analytical methods. Conclusions This method is an important tool for the identification and quantification of major constituents in QSYQ. Highlights Compared with traditional HPLC, the established qHNMR method has the advantages of simple sample preparation, short analysis time, and non-destructive analysis.

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