77. Human Adipose-Derived Stromal Cells Elaborate and Respond to Bone Morphogenetic Protein-2 (BMP-2) During in Vitro Osteogenic Differentiation

2009 ◽  
Vol 151 (2) ◽  
pp. 206
Author(s):  
D.M. Gupta ◽  
N.J. Panetta ◽  
M.D. Kwan ◽  
N. Quarto ◽  
M.T. Longaker
Keyword(s):  
Osteogenic Differentiation ◽  
Bone Morphogenetic Protein ◽  
Stromal Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Adipose Derived Stromal Cells ◽  
Morphogenetic Protein

Development of osteoplastic Material Impregnated with Plasmid Encoding Bone Morphogenetic Protein-2

2020 ◽  
Vol 36 (4) ◽  
pp. 59-64
Author(s):  
T.B. Bukharova ◽  
Yu.D. Zagoskin ◽  
A.V. Vasilyev ◽  
T.E. Grigoriev ◽  
V.O. Mokrousova ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Biological Properties ◽  
Bone Morphogenetic Protein 2 ◽  
Russian Science ◽  
Morphogenetic Protein ◽  
Osteoplastic Material

The biological properties of a new gene-activated osteoplastic material based on chitosan sponges impregnated with plasmids with the gene for the bone morphogenetic protein-2 (BMP2) have been studied. The optimal conditions for transfection of multipotent mesenchymal stromal cells with TurboFect reagent were selected. The in vitro osteoinducing effect of the developed material associated with an increase in the expression of osteogenic markers in mesenchymal stromal cells was shown. The designed gene-activated material can be used to repair bone defects. gene-activated material, plasmids, BMP-2, gene therapy, bone regeneration The work was supported by the Russian Science Foundation (Grant no. 16-15-00298).

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