Development of osteoplastic Material Impregnated with Plasmid Encoding Bone Morphogenetic Protein-2

2020 ◽  
Vol 36 (4) ◽  
pp. 59-64
Author(s):  
T.B. Bukharova ◽  
Yu.D. Zagoskin ◽  
A.V. Vasilyev ◽  
T.E. Grigoriev ◽  
V.O. Mokrousova ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Biological Properties ◽  
Bone Morphogenetic Protein 2 ◽  
Russian Science ◽  
Morphogenetic Protein ◽  
Osteoplastic Material

The biological properties of a new gene-activated osteoplastic material based on chitosan sponges impregnated with plasmids with the gene for the bone morphogenetic protein-2 (BMP2) have been studied. The optimal conditions for transfection of multipotent mesenchymal stromal cells with TurboFect reagent were selected. The in vitro osteoinducing effect of the developed material associated with an increase in the expression of osteogenic markers in mesenchymal stromal cells was shown. The designed gene-activated material can be used to repair bone defects. gene-activated material, plasmids, BMP-2, gene therapy, bone regeneration The work was supported by the Russian Science Foundation (Grant no. 16-15-00298).

DEVELOPMENT OF OSTEOPLASTIC MATERIAL IMPREGNATED WITH PLASMID ENCODING BONE MORPHOGENETIC PROTEIN-2

2020 ◽  
pp. 63-65
Author(s):  
T. Bukharova ◽  
A. Vasilyev ◽  
A. Kulakov ◽  
D. Goldstein
Keyword(s):  
Bone Morphogenetic Protein ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
New Gene ◽  
Osteoplastic Material

A new gene-activated osteoplastic based on chitosan material impregnated with plasmid with the BMP-2 gene has been developed. The material had an osteoinductive effect on mesenchymal stromal cells (MSC).

Enhanced cytocompatibility and osteoinductive properties of sol–gel-derived silica/zirconium dioxide coatings by metformin functionalization

2017 ◽  
Vol 32 (5) ◽  
pp. 570-586 ◽  
Author(s):  
Agnieszka Śmieszek ◽  
Joanna Szydlarska ◽  
Aleksandra Mucha ◽  
Martyna Chrapiec ◽  
Krzysztof Marycz
Keyword(s):  
Adipose Tissue ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Messenger Rna ◽  
Zirconium Dioxide ◽  
Sol Gel ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Matrix Mineralization

The aim of this study was to evaluate the pro-osteogenic properties of sol–gel-derived silica/zirconium dioxide coatings functionalized with 1 mM of metformin. The matrices were applied on 316L stainless steel using dip-coating technique. First of all, physicochemical properties of biomaterials were evaluated. Surface morphology and topography was determined using energy-dispersive X-ray spectroscopy and atomic force microscopy. The chemical composition was evaluated using Fourier transform infrared spectroscopy. Further, wettability and surface free energy were characterized. Cytocompatibility of biomaterials was tested in vitro using model of human multipotent mesenchymal stromal cells isolated from adipose tissue. The influence of biomaterials on cells morphology and proliferation was determined. Osteogenic effect of obtained biomaterials was evaluated in terms of their influence on secretory activity of human multipotent mesenchymal stromal cells isolated from adipose tissue and matrix mineralization. Analysis was performed in relation to the control cultures i.e. maintained on pure SS316L substrate and SS316L covered with silica/zirconium dioxide. Obtained results indicate that silica/zirconium dioxide_metformin coatings ameliorated metabolic and proliferative activity of human multipotent mesenchymal stromal cells isolated from adipose tissue, as well as promoted their proper growth and adhesion. The human multipotent mesenchymal stromal cells isolated from adipose tissue cultured on biomaterials were characterized by typical fibroblast-like morphology. The addition of metformin to the silica/zirconium dioxide coatings improved functional differentiation of human multipotent mesenchymal stromal cells isolated from adipose tissue. Osteogenic cultures on silica/zirconium dioxide_metformin were characterized by formation of well-developed osteonodules rich in calcium and phosphorous. Moreover, human multipotent mesenchymal stromal cells isolated from adipose tissue cultured on silica/zirconium dioxide_metformin synthesized increased amount of alkaline phosphatase, bone morphogenetic protein 2 and osteopontin, both on messenger RNA and protein level. Obtained biomaterials modulate cellular plasticity of human multipotent mesenchymal stromal cells isolated from adipose tissue promoting their osteogenic differentiation, thus may find application in broadly defined tissue engineering.

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