Anti-melanoma action of small molecular peptides derived from Brucea javanica(L.)Merr. globulin in vitro

Pancreatic cancer arises when cells in the pancreas begin to multiply out of control. In pancreatic cancer, over expression of heat proteins (Hsp70, Hsp 90), constitutive activation of NFĸB, and Bcl-2 family are closely linked with resistance to apoptosis. Apoptotic resistance has been attributed to defects in apoptotic signaling pathways. Bruceine D, which found in abundance Brucea javanica, possesses potent anti-pancreatic cancer activity. In vitro result, bruceine D could induce apoptosis of pancreatic cancer cell. The aim of this study was to find the potential effect of bruceine D inhibitor on apoptotic resistance proteins in pancreatic cancer based on molecular docking. Docking showed a binding affinity between bruceine D with proteins involved in apoptosis using AutoDock. The results showed that free binding energy of Hsp70 is -5.19; Hsp90 -7.26; NFĸB1 -5.49; NFĸB2 -6.14; Bcl-W -6.02; Bcl-xL -5.45 kcal/mol. Based on the result, we conclude that bruceine D with Hsp90 protein has potential the best binding affinity than other proteins.

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The Potential Anti-Inflammatory Effects of Brucea javanica (L.) Merr.

International Journal of Pharmaceutical Sciences and Medicine ◽

10.47760/ijpsm.2021.v06i09.001 ◽

2021 ◽

Vol 6 (9) ◽

pp. 1-7

Author(s):

Sisi Mustika ◽

Sri Oktavia ◽

Ifora Ifora

Keyword(s):

Mrna Expression ◽

Initial Response ◽

In Vivo Studies ◽

Brucea Javanica ◽

Ppar Γ ◽

Pharmacological Studies ◽

Ifn Γ ◽

Anti Inflammatory

Inflammation is the initial response to acute and chronic tissue damage, which is characterized by redness, swelling, heat, and pain. Natural products derived from plants have specific pharmacological activity and minimal side effects. Brucea javanica is a plant that has an anti-inflammatory effect, this plant contains alkaloid and flavonoid compounds. Flavonoids have the ability to block cyclooxygenase and lipoxygenase while alkaloids as an anti-inflammatory are thought to work by inhibiting prostaglandin H2 PGH2 which is an inflammatory mediator. From the data obtained, there is no complete literature that reviews its use as an anti-inflammatory. The search databases used are as follows: Pubmed, ScienceDirect, and Google Scholar to study the anti-inflammatory activity of Brucea javanica. All recent research articles were published between 2010 to 2021. Based on eligibility, 4 studies were included in this study, consisting of 2 In vivo studies and 2 In vitro and In vivo studies. A series of pharmacological studies have reported that Brucea javanica can block the Nf-kB signaling pathway and decrease the production of inflammatory mediators. It has been reported to be able to inhibit the production of NO, PGE2, TNF-, IL-1β, IL-18IL-23, COX-2, NF-κB, IFN-γ, IL-6, the levels of MPO (Myeloperoxidase), reducing the edema and induce the production of the anti-inflammatory cytokine (IL-4, IL-10 and TGF-β). Brucea javanica also markedly activates Nrf2 expression suppressing the inflammatory response-mediated NLRP3 and NF-κB activation. In addition, the elevated mRNA expression of MMP-1, MMP-3 and RAGE was remarkably inhibited by Brucea javanica, while the mRNA expression of PPAR-γ was significantly enhanced. In vitro and in vivo studies strongly indicate that Brucea javanica has the potential as an anti-inflammatory.

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Anti-babesial Quassinoids from the Fruits of Brucea Javanica

Natural Product Communications ◽

10.1177/1934578x0800300207 ◽

2008 ◽

Vol 3 (2) ◽

pp. 1934578X0800300 ◽

Cited By ~ 1

Author(s):

Ahmed Elkhateeb ◽

Masahiro Yamasaki ◽

Yoshimitsu Maede ◽

Ken Katakura ◽

Kensuke Nabeta ◽

...

Keyword(s):

Medicinal Plant ◽

Spectral Data ◽

Chloroform Extract ◽

Mass Spectral Data ◽

Babesia Gibsoni ◽

Diminazene Aceturate ◽

Active Compounds ◽

Mass Spectral ◽

Brucea Javanica

The medicinal plant Brucea javanica (L.) Merr. (Simaroubaceae), grown in China, was examined for anti-babesial properties. The anti-babesial activity of the fruit was found to be attributed to its quassinoid constituents. Ten active compounds were isolated and purified from a chloroform extract. The identities of these compounds were confirmed from NMR spectroscopic and mass spectral data as brusatol (1), bruceantin (2), bruceine A (3), bruceantinol (4), dehydrobruceine B (5), dehydrobrusatol (6), dehydrobruceine A (7), bruceine D (8), bruceoside A (9), and yadanzioside G (10). When tested in vitro against Babesia gibsoni, compounds 1–10 had IC50 values of 0.74, 13.4, 4.0, 12.0, 308.2, 10.5, 835.0, >1000, and >1000 ng/mL, respectively. Compounds 1–4, 6 and 7 had far higher activity than the commercial anti-babesial drug diminazene aceturate, which possesses an IC50 value of 70.5 ng/mL. Except for bruceine A (3), bruceantinol (4) and bruceine D (8), this is the first report of the anti-babesial activity of these isolated quassinoids.

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