Comparison of ELISA for the detection of porcine serum antibodies to non-structural proteins of foot-and-mouth disease virus

2004 ◽  
Vol 116 (2) ◽  
pp. 155-159 ◽  
Author(s):  
Fan Lee ◽  
Yeou-Liang Lin ◽  
Ming-Hwa Jong
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Proteins ◽  
Serum Antibodies ◽  
Porcine Serum ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Proteomics Analysis of Porcine Serum Proteins by LC-MS/MS after Foot-and-Mouth Disease Virus (FMDV) Infection

2011 ◽  
Vol 73 (12) ◽  
pp. 1569-1572 ◽  
Author(s):  
Yongjie LIU ◽  
Keshan ZHANG ◽  
Haixue ZHENG ◽  
Youjun SHANG ◽  
Jianhong GUO ◽  
...  
Keyword(s):  
Disease Virus ◽  
Serum Proteins ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Proteomics Analysis ◽  
Porcine Serum ◽  
Fmdv Infection ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Infection and protection responses of deletion mutants of non-structural proteins of foot-and-mouth disease virus serotype Asia1 in guinea pigs

2021 ◽  
Author(s):  
H. Lalzampuia ◽  
Subhadra Elango ◽  
Jitendra K. Biswal ◽  
Narayanan Krishnaswamy ◽  
R. P. Tamil Selvan ◽  
...  
Keyword(s):  
Disease Virus ◽  
Guinea Pigs ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Proteins ◽  
Deletion Mutants ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Foot And Mouth

Screening and identification of B cell epitopes of structural proteins of foot-and-mouth disease virus serotype Asia1

2010 ◽  
Vol 140 (1-2) ◽  
pp. 25-33 ◽  
Author(s):  
Zhong-Wang Zhang ◽  
Yong-Guang Zhang ◽  
Yong-Lu Wang ◽  
Li Pan ◽  
Yu-Zhen Fang ◽  
...  
Keyword(s):  
B Cell ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Proteins ◽  
B Cell Epitopes ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Foot And Mouth ◽  
Screening And Identification

scholarly journals An improved, rapid competitive ELISA using a novel conserved 3B epitope for the detection of serum antibodies to foot-and-mouth disease virus

2018 ◽  
Vol 30 (5) ◽  
pp. 699-707 ◽  
Author(s):  
Chungwon J. Chung ◽  
Alfonso Clavijo ◽  
Mangkey A. Bounpheng ◽  
Sabena Uddowla ◽  
Abu Sayed ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Competitive Elisa ◽  
Mouth Disease ◽  
Post Inoculation ◽  
Serum Antibodies ◽  
Control Serum ◽  
Fmdv Serotypes ◽  
Mouth Disease Virus ◽  
Foot And Mouth

The highly contagious foot-and-mouth disease virus (FMDV) afflicts cloven-hoofed animals, resulting in significant costs because of loss of trade and recovery from disease. We developed a sensitive, specific, and rapid competitive ELISA (cELISA) to detect serum antibodies to FMDV. The cELISA utilized a monoclonal blocking antibody specific for a highly conserved FMDV nonstructural 3B epitope, a recombinant mutant FMDV 3ABC coating protein, and optimized format variables including serum incubation for 90 min at 20–25°C. Samples from 16 animals experimentally infected with one FMDV serotype (A, O, Asia, or SAT-1) demonstrated early detection capacity beginning 7 d post-inoculation. All samples from 55 vesicular stomatitis virus antibody-positive cattle and 44 samples from cloven-hoofed animals affected by non-FMD vesicular diseases were negative in the cELISA, demonstrating 100% analytical specificity. The diagnostic sensitivity was 100% against sera from 128 cattle infected with isolates of all FMDV serotypes, emphasizing serotype-agnostic results. Diagnostic specificities of U.S. cattle ( n = 1135) and swine ( n = 207) sera were 99.4% and 100%, respectively. High repeatability and reproducibility were demonstrated with 3.1% coefficient of variation in percent inhibition data and 100% agreement using 2 kit lots and 400 negative control serum samples, with no difference between bench and biosafety cabinet operation. Negative results from vaccinated, uninfected cattle, pig, and sheep sera confirmed the DIVA (differentiate infected from vaccinated animals) capability. This rapid (<3 h), select agent–free assay with high sensitivity and specificity, DIVA capability, and room temperature processing capability will serve as a useful tool in FMDV surveillance, emergency preparedness, response, and outbreak recovery programs.

scholarly journals Detection and Characterization of Functional T-Cell Epitopes on the Structural Proteins VP2, VP3, and VP4 of Foot and Mouth Disease Virus O1 Campos

Virology ◽  
2000 ◽  
Vol 271 (2) ◽  
pp. 234-239 ◽  
Author(s):  
M. Pérez Filgueira ◽  
A. Wigdorovitz ◽  
A. Romera ◽  
P. Zamorano ◽  
M.V. Borca ◽  
...  
Keyword(s):  
T Cell ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Proteins ◽  
T Cell Epitopes ◽  
Mouth Disease Virus ◽  
Foot And Mouth

The nucleotide sequence of cDNA coding for the structural proteins of foot-and-mouth disease virus

Gene ◽  
1982 ◽  
Vol 17 (2) ◽  
pp. 153-161 ◽  
Author(s):  
J.C. Boothroyd ◽  
T.J.R. Harris ◽  
D.J. Rowlands ◽  
P.A. Lowe
Keyword(s):  
Nucleotide Sequence ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Proteins ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Development of a Luminex assay for the detection of swine antibodies to non-structural proteins of foot-and-mouth disease virus

2013 ◽  
Vol 396 (1-2) ◽  
pp. 87-95 ◽  
Author(s):  
Tsu-Han Chen ◽  
Fan Lee ◽  
Yeou-Liang Lin ◽  
Chu-Hsiang Pan ◽  
Chia-Ni Shih ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Proteins ◽  
Luminex Assay ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Chromatographic preparation of purified structural proteins from foot-and-mouth disease virus

1974 ◽  
Vol 58 (3) ◽  
pp. 624-632 ◽  
Author(s):  
S. Bernard ◽  
Josiane Wantyghem ◽  
Jeanne Grosclaude ◽  
J. Laporte
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Proteins ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Cellular and humoral immunity to foot-and-mouth disease virus and its non-structural proteins in infected swine

1997 ◽  
Vol 25 (2) ◽  
pp. 273S-273S
Author(s):  
A. COOK ◽  
H. ANGOVE ◽  
L. CEDILLO-BARRON ◽  
M. FOSTER-CUEVAS ◽  
R.M.E. PARKHOUSE
Keyword(s):  
Disease Virus ◽  
Humoral Immunity ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Proteins ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Cellular And Humoral Immunity
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