Differentiation of Italian field and South African vaccine strains of bluetongue virus serotype 2 using real-time PCR

2004 ◽  
Vol 122 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Germano Orrù ◽  
Paola De Santis ◽  
Fabio Solinas ◽  
Giovanni Savini ◽  
Vincenzo Piras ◽  
...  
Keyword(s):  
Real Time ◽  
South African ◽  
Real Time Pcr ◽  
Bluetongue Virus ◽  
Virus Serotype

Detection and Quantification of Bluetongue Virus Serotype 23 by Evagreen Based Real Time PCR

2018 ◽  
Vol 8 (5) ◽  
pp. 312
Author(s):  
Shabir Bhat ◽  
Jaynudin Khorajiya ◽  
Bilal Malla ◽  
Pervaiz Dar ◽  
Karam Singh ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bluetongue Virus ◽  
Virus Serotype ◽  
Detection And Quantification

Detection of the European ‘field’ strain of bluetongue virus serotype 6 by real-time RT-PCR

2010 ◽  
Vol 141 (1-2) ◽  
pp. 186-188 ◽  
Author(s):  
C.A. Batten ◽  
P.A. van Rijn ◽  
C.A.L. Oura
Keyword(s):  
Real Time ◽  
Bluetongue Virus ◽  
Field Strain ◽  
Rt Pcr ◽  
Virus Serotype

Real time PCR of Nor~1 (aflD) gene of aflatoxin producing fungi and its correlative quantization to aflatoxin levels in South African compound feeds

2014 ◽  
Vol 97 ◽  
pp. 63-67 ◽  
Author(s):  
H.E. Iheanacho ◽  
M.F. Dutton ◽  
P.A. Steenkamp ◽  
L. Steenkamp ◽  
H.A. Makun ◽  
...  
Keyword(s):  
Real Time ◽  
South African ◽  
Real Time Pcr

Vaccinia virus expression of the VP7 protein of South African bluetongue virus serotype 4 and its use as an antigen in a capture ELISA

1994 ◽  
Vol 135 (3-4) ◽  
pp. 405-418 ◽  
Author(s):  
M. Cloete ◽  
D. H. du Plessis ◽  
A. A. van Dijk ◽  
H. Huismans ◽  
G. J. Viljoen
Keyword(s):  
Vaccinia Virus ◽  
South African ◽  
Bluetongue Virus ◽  
Capture Elisa ◽  
Virus Serotype ◽  
Vp7 Protein ◽  
Virus Expression

Universal Real-Time PCR for the Detection and Quantification of Adeno-Associated Virus Serotype 2-Derived Inverted Terminal Repeat Sequences

2011 ◽  
pp. 110805114044008 ◽  
Author(s):  
Christine Aurnhammer ◽  
Maren Haase ◽  
Nadine Muether ◽  
Martin Hausl ◽  
Christina Rauschhuber ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Terminal Repeat ◽  
Inverted Terminal Repeat ◽  
Adeno Associated Virus ◽  
Repeat Sequences ◽  
Virus Serotype ◽  
Detection And Quantification

scholarly journals Real time PCR. Application in dengue studies

2011 ◽  
pp. 243-258 ◽  
Author(s):  
Jeanette Prada-Arismendy ◽  
Jaime E Castellanos
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Dengue Virus Infection ◽  
Virus Serotype ◽  
Human Genome Sequencing ◽  
Polymerase Chain ◽  
Detection Of Mutations ◽  
Dengue Virus Serotype 2

PCR (polymerase chain reaction) is a routinely used tool in every diagnostic and research laboratory. This technique has been used in detection of mutations and pathogens, forensic investigation, and even is the base tool for human genome sequencing. A modification of PCR technique, real time PCR, allows the quantification of nucleic acids with higher sensibility, specificity and reproducibility. This article is intended to clarify the foundations of real-time PCR, using an application model for virology. In the actual work, it was quantified the viral load of dengue virus serotype 2 produced from infected murine macrophages; the obtained results in this work established that murine strain BALB/c presents a greater susceptibility to dengue virus infection, which establishes BALB/c murine strain as a best model of study for investigation of dengue virus infection physiopathology.

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