Vaccinia virus expression of the VP7 protein of South African bluetongue virus serotype 4 and its use as an antigen in a capture ELISA

1994 ◽  
Vol 135 (3-4) ◽  
pp. 405-418 ◽  
Author(s):  
M. Cloete ◽  
D. H. du Plessis ◽  
A. A. van Dijk ◽  
H. Huismans ◽  
G. J. Viljoen
Keyword(s):  
Vaccinia Virus ◽  
South African ◽  
Bluetongue Virus ◽  
Capture Elisa ◽  
Virus Serotype ◽  
Vp7 Protein ◽  
Virus Expression

Differentiation of Italian field and South African vaccine strains of bluetongue virus serotype 2 using real-time PCR

2004 ◽  
Vol 122 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Germano Orrù ◽  
Paola De Santis ◽  
Fabio Solinas ◽  
Giovanni Savini ◽  
Vincenzo Piras ◽  
...  
Keyword(s):  
Real Time ◽  
South African ◽  
Real Time Pcr ◽  
Bluetongue Virus ◽  
Virus Serotype

High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System

1992 ◽  
Vol 68 (02) ◽  
pp. 119-124 ◽  
Author(s):  
F G Falkner ◽  
P L Turecek ◽  
R T A MacGillivray ◽  
W Bodemer ◽  
F Scheiflinger ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Cell Lines ◽  
Expression System ◽  
Human Cell Line ◽  
Cell System ◽  
Time Saving ◽  
Expression Levels ◽  
Mammalian Cell Lines ◽  
Cell Line Hela ◽  
Virus Expression

SummaryWe have worked out an efficient and time saving procedure for the expression of recombinant human prothrombin. The glycoprotein was expressed in the vaccinia virus expression system in several mammalian cell lines. The kidney cell lines Vero and BHK and the human cell line Hela were found to efficiently secrete prothrombin. Expression levels of 3–4 µg of factor II per 106 cells per day corresponding to 18–23 mU per 106 cells per day were achieved. Since the expression levels obtained with the vaccinia virus/Vero cell system were comparable to those obtained in amplified transformed CHO cells it provides an alternative system for the efficient expression of human prothrombin and may allow to further elucidate structure-function relationships of (pro)thrombin and its various effectors.

A multi-species indirect ELISA for detection group-specific antibodies against VP7 protein of bluetongue virus

2019 ◽  
Vol 180 ◽  
pp. 6-8 ◽  
Author(s):  
Karam Chand ◽  
Sanchay Kumar Biswas ◽  
Muthannan Andavar Ramakrishnan
Keyword(s):  
Bluetongue Virus ◽  
Indirect Elisa ◽  
Specific Antibodies ◽  
Vp7 Protein

Laboratory and field studies of an antigen capture ELISA for bluetongue virus

2000 ◽  
Vol 85 (1-2) ◽  
pp. 137-149 ◽  
Author(s):  
R.A Hawkes ◽  
P.D Kirkland ◽  
D.A Sanders ◽  
F Zhang ◽  
Z Li ◽  
...  
Keyword(s):  
Bluetongue Virus ◽  
Field Studies ◽  
Capture Elisa ◽  
Antigen Capture Elisa ◽  
Antigen Capture
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