In situ hybridization assays for localization of the chronic bee paralysis virus in the honey bee (Apis mellifera) brain

2008 ◽  
Vol 153 (2) ◽  
pp. 232-237 ◽  
Author(s):  
Violaine Olivier ◽  
Isabelle Massou ◽  
Olivier Celle ◽  
Philippe Blanchard ◽  
Frank Schurr ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Apis Mellifera ◽  
Honey Bee ◽  
Paralysis Virus ◽  
Hybridization Assays

scholarly journals Clinical Utility of In Situ Hybridization Assays in Head and Neck Neoplasms

2018 ◽  
Vol 13 (3) ◽  
pp. 397-414
Author(s):  
Peter P. Luk ◽  
Christina I. Selinger ◽  
Wendy A. Cooper ◽  
Annabelle Mahar ◽  
Carsten E. Palme ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Head And Neck ◽  
Head And Neck Neoplasms ◽  
Clinical Utility ◽  
Hybridization Assays

scholarly journals A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kerstin Seitz ◽  
Katharina Buczolich ◽  
Alžbeta Dikunová ◽  
Pavel Plevka ◽  
Karen Power ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Clinical Symptoms ◽  
Well Being ◽  
Reverse Genetic System ◽  
Progeny Virus ◽  
Molecular Clone ◽  
Depth Analysis ◽  
Paralysis Virus

Abstract Among the many diseases compromising the well-being of the honey bee (Apis mellifera) the chronic paralysis syndrome of adult honey bees is one of the best described. The causative agent, chronic bee paralysis virus (CBPV), is a positive sense, single-stranded RNA virus with a segmented genome. Segment 1 encodes three putative open reading frames (ORFs), including the RNA-dependent RNA polymerase and other non-structural protein coding regions. Segment 2 encodes four putative ORFs, which contain the genes of supposed structural proteins. In this study, we established a reverse genetic system for CBPV by molecular cloning of DNA copies of both genome segments. CBPV rescue was studied in imago and honey bee pupae infection models. Virus replication and progeny virus production was only initiated when capped RNAs of both genome segments were injected in honey bees. As injection of these clonal RNAs caused clinical symptoms similar to wild-type CBPV infection, we conclude that the novel molecular clone fulfilled Koch’s postulates. Our virus clone will enable in-depth analysis of CBPV pathogenesis and help to increase knowledge about this important honey bee disease.

scholarly journals Preclinical validation of fluorescence in situ hybridization assays for clinical practice

2006 ◽  
Vol 8 (1) ◽  
pp. 16-23 ◽  
Author(s):  
Anne E Wiktor ◽  
Daniel L Van Dyke ◽  
Peggy J Stupca ◽  
Rhett P Ketterling ◽  
Erik C Thorland ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Clinical Practice ◽  
Fluorescence In Situ Hybridization ◽  
Hybridization Assays

scholarly journals Development of Five Dual-Color, Double-Fusion Fluorescence in Situ Hybridization Assays for the Detection of Common MLL Translocation Partners

2010 ◽  
Vol 12 (4) ◽  
pp. 441-452 ◽  
Author(s):  
Jeannette G. Keefe ◽  
William R. Sukov ◽  
Ryan A. Knudson ◽  
Lai P. Nguyen ◽  
Cynthia Williamson ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dual Color ◽  
Hybridization Assays

In-situ Hybridization for the Detection of Sacbrood Virus in Infected Larvae of the Honey Bee (Apis cerana)

2016 ◽  
Vol 154 (2-3) ◽  
pp. 258-262 ◽  
Author(s):  
C. Park ◽  
H.S. Kang ◽  
J. Jeong ◽  
I. Kang ◽  
K. Choi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Honey Bee ◽  
Apis Cerana ◽  
Sacbrood Virus

scholarly journals OCCURRENCE OF ACUTE PARALYSIS VIRUS OF THE HONEY BEE (APIS MELLIFERA) IN A HUNGARIAN APIARY INFESTED WITH THE PARASITIC MITE VARROA JACOBSONI

1999 ◽  
Vol 47 (3) ◽  
pp. 319-324 ◽  
Author(s):  
L. Békési ◽  
Brenda V. Ball ◽  
M. Dobos-Kovács ◽  
T. Bakonyi ◽  
M. Rusvai
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Clinical Symptoms ◽  
Varroa Jacobsoni ◽  
Infected Adult ◽  
Long Time ◽  
Parasitic Mite ◽  
Ultrathin Sections ◽  
The Relationship ◽  
Paralysis Virus

Viruses of the honey bee have been known for a long time; however, recently the attention of scientists and apiculturalists has turned towards the relationship between these viruses and the parasitic miteVarroa jacobsoni. Although clinical symptoms indicated the presence of some of the viruses of bees in Hungary, none have previously been isolated or identified. During July unusual adult bee and brood mortality was observed in some colonies of an apiary in Budapest known to be infested withVarroa jacobsoni. Large amounts of acute paralysis virus (APV) were detected serologically in healthy honey bee pupae killed by the injection of a bacteria-free extract of diseased adult bees. Crystalline arrays of 30 nm particles were seen in ultrathin sections of the tissues of injected pupae and naturally infected adult bees. In spite of the application of acaricide treatments the bee population in several colonies had collapsed by the end of summer and the apiary suffered severe wintering losses.

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