Development of a hamster kidney cell line expressing stably T7 RNA polymerase using retroviral gene transfer technology for efficient rescue of infectious foot-and-mouth disease virus

The fetal goat tongue cell line ZZ-R 127 and the fetal porcine kidney cell line LFBK-αvβ6 have been reported to have high sensitivity to various Foot-and-mouth disease virus (FMDV) strains. The suitability of ZZ-R 127 cells for FMDV isolation not only from epithelial suspensions but also from other clinical samples has already been confirmed in a previous study. However, to our knowledge, the suitability of LFBK-αvβ6 cells has not been evaluated using clinical samples other than epithelial materials. In addition, both cell lines have never been compared, in terms of use for FMDV isolation, under the same conditions. Therefore, in the current study, the virus isolation rates of both cell lines were compared using clinical samples collected from animals infected experimentally with FMDV. Viruses were successfully isolated from clinical samples other than epithelial suspensions for both cell lines. The virus isolation rates for the 2 cell lines were not significantly different. The Cohen kappa coefficients between the virus isolation results for both cell lines were significantly high. Taken together, these results confirmed the suitability of LFBK-αvβ6 cells for FMDV isolation from clinical samples other than epithelial suspensions. The levels of susceptibility of both cell lines to FMDV isolation were also confirmed to be almost the same.

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Recovery of infectious classical swine fever virus (CSFV) from full-length genomic cDNA clones by a swine kidney cell line expressing bacteriophage T7 RNA polymerase

Journal of Virological Methods ◽

10.1016/s0166-0934(98)00171-2 ◽

1999 ◽

Vol 78 (1-2) ◽

pp. 117-128 ◽

Cited By ~ 27

Author(s):

H.G.P. van Gennip ◽

P.A. van Rijn ◽

M.N. Widjojoatmodjo ◽

R.J.M. Moormann

Keyword(s):

Rna Polymerase ◽

Cell Line ◽

Classical Swine Fever Virus ◽

Kidney Cell ◽

Classical Swine Fever ◽

T7 Rna Polymerase ◽

Cdna Clones ◽

Kidney Cell Line ◽

Genomic Cdna ◽

Swine Kidney

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Factors Required for the Uridylylation of the Foot-and-Mouth Disease Virus 3B1, 3B2, and 3B3 Peptides by the RNA-Dependent RNA Polymerase (3Dpol) In Vitro

Journal of Virology ◽

10.1128/jvi.79.12.7698-7706.2005 ◽

2005 ◽

Vol 79 (12) ◽

pp. 7698-7706 ◽

Cited By ~ 57

Author(s):

Arabinda Nayak ◽

Ian G. Goodfellow ◽

Graham J. Belsham

Keyword(s):

Rna Polymerase ◽

Disease Virus ◽

Rna Synthesis ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Coding Region ◽

Mouth Disease Virus ◽

Foot And Mouth ◽

Positive Strand Rna

ABSTRACT The 5′ terminus of picornavirus genomic RNA is covalently linked to the virus-encoded peptide 3B (VPg). Foot-and-mouth disease virus (FMDV) is unique in encoding and using 3 distinct forms of this peptide. These peptides each act as primers for RNA synthesis by the virus-encoded RNA polymerase 3Dpol. To act as the primer for positive-strand RNA synthesis, the 3B peptides have to be uridylylated to form VPgpU(pU). For certain picornaviruses, it has been shown that this reaction is achieved by the 3Dpol in the presence of the 3CD precursor plus an internal RNA sequence termed a cis-acting replication element (cre). The FMDV cre has been identified previously to be within the 5′ untranslated region, whereas all other picornavirus cre structures are within the viral coding region. The requirements for the in vitro uridylylation of each of the FMDV 3B peptides has now been determined, and the role of the FMDV cre (also known as the 3B-uridylylation site, or bus) in this reaction has been analyzed. The poly(A) tail does not act as a significant template for FMDV 3B uridylylation.

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