porcine kidney cell
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2019 ◽  
Vol 11 (1) ◽  
pp. 61-69
Author(s):  
Gospava Lazić ◽  
Tamaš Petrović ◽  
Diana Lupulović ◽  
Bojan Topalski ◽  
Biljana Božić ◽  
...  

This article reports on the occurrence and diagnosis of Aujeszky’s disease in a dog. The procedure for isolation and identification of Aujeszky’sdisease virus was described. A dog of unknown breed aged about two years died of Aujeszky’s disease after consuming animal offal (internal organs: lungs, spleen, kidneys) fed by the owner after slaughtering piglets and preparing meat for cooking. As early as 24 hours after consuming the offal, the dog manifested characteristic symptoms of Aujeszky’s disease, which were immediately recognized by the veterinarian. The death occurred within less than 24 hours upon first clinical signs of disease. Aujeszky’s disease virus was isolated and identified from brain and internal organ (lung and spleen) samples of the dog at the Department of Virology of the Scientific Veterinary Institute „Novi Sad“. Isolation and identification of the virus was performed on PK-15 porcine kidney cell line and using nested PCR technique.



2015 ◽  
Vol 27 (4) ◽  
pp. 516-521 ◽  
Author(s):  
Katsuhiko Fukai ◽  
Kazuki Morioka ◽  
Manabu Yamada ◽  
Tatsuya Nishi ◽  
Kazuo Yoshida ◽  
...  

The fetal goat tongue cell line ZZ-R 127 and the fetal porcine kidney cell line LFBK-αvβ6 have been reported to have high sensitivity to various Foot-and-mouth disease virus (FMDV) strains. The suitability of ZZ-R 127 cells for FMDV isolation not only from epithelial suspensions but also from other clinical samples has already been confirmed in a previous study. However, to our knowledge, the suitability of LFBK-αvβ6 cells has not been evaluated using clinical samples other than epithelial materials. In addition, both cell lines have never been compared, in terms of use for FMDV isolation, under the same conditions. Therefore, in the current study, the virus isolation rates of both cell lines were compared using clinical samples collected from animals infected experimentally with FMDV. Viruses were successfully isolated from clinical samples other than epithelial suspensions for both cell lines. The virus isolation rates for the 2 cell lines were not significantly different. The Cohen kappa coefficients between the virus isolation results for both cell lines were significantly high. Taken together, these results confirmed the suitability of LFBK-αvβ6 cells for FMDV isolation from clinical samples other than epithelial suspensions. The levels of susceptibility of both cell lines to FMDV isolation were also confirmed to be almost the same.



2013 ◽  
Vol 17 (5) ◽  
pp. 341-347 ◽  
Author(s):  
Ju-Taek Lee ◽  
Kisung Ko ◽  
Kyung Jin Lee ◽  
Malg-Um Lim ◽  
Ghislain Moussavou ◽  
...  


2013 ◽  
Vol 176 (1-2) ◽  
pp. 292-297 ◽  
Author(s):  
Yungang Lan ◽  
Kui Zhao ◽  
Gaili Wang ◽  
Bo Dong ◽  
Jiakuan Zhao ◽  
...  


2013 ◽  
Vol 187 (2) ◽  
pp. 380-383 ◽  
Author(s):  
Hsu-Chung Gabriel Chen ◽  
Tsun-Yung Kuo ◽  
Ying-Chen Yang ◽  
Chung-Chin Wu ◽  
Shiow-Suey Lai






2003 ◽  
Vol 77 (13) ◽  
pp. 7645-7654 ◽  
Author(s):  
Nicolas Ruggli ◽  
Jon-Duri Tratschin ◽  
Matthias Schweizer ◽  
Kenneth C. McCullough ◽  
Martin A. Hofmann ◽  
...  

ABSTRACT Classical swine fever virus (CSFV) replicates efficiently in cell lines and monocytic cells, including macrophages (MΦ), without causing a cytopathic effect or inducing interferon (IFN) secretion. In the present study, the capacity of CSFV to interfere with cellular antiviral activity was investigated. When the porcine kidney cell line SK-6 was infected with CSFV, there was a 100-fold increased capacity to resist to apoptosis induced by polyinosinic-polycytidylic acid [poly(IC)], a synthetic double-stranded RNA. In MΦ, the virus infection inhibited poly(IC)-induced alpha/beta IFN (type I IFN) synthesis. This interference with cellular antiviral defense correlated with the presence of the viral Npro gene. Mutants lacking the Npro gene (ΔNpro CSFV) did not protect SK-6 cells from poly(IC)-induced apoptosis, despite growth properties and protein expression levels similar to those of the wild-type virus. Furthermore, ΔNpro CSFV did not prevent poly(IC)-induced type I IFN production in MΦ but rather induced type I IFN in the absence of poly(IC) in both MΦ and the porcine kidney cell line PK-15, but not in SK-6 cells. With MΦ and PK-15, an impaired replication of the ΔNpro CSFV compared with wild-type virus was noted. In addition, ΔNpro CSFV, but not wild-type CSFV, could interfere with vesicular stomatitis virus replication in PK-15 cells. Taken together, these results provide evidence for a novel function associated with CSFV Npro with respect to the inhibition of the cellular innate immune system.



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