Expression and antigenic characterization of bubaline herpesvirus 1 (BuHV1) glycoprotein E and its potential application in the epidemiology and control of alphaherpesvirus infections in Mediterranean water buffalo

2014 ◽  
Vol 207 ◽  
pp. 16-21 ◽  
Author(s):  
C. Nogarol ◽  
L. Bertolotti ◽  
E. De Carlo ◽  
L. Masoero ◽  
C. Caruso ◽  
...  
2013 ◽  
Vol 94 (3) ◽  
pp. 813-816 ◽  
Author(s):  
M.G. Amoroso ◽  
F. Corrado ◽  
E. De Carlo ◽  
M.G. Lucibelli ◽  
A. Martucciello ◽  
...  

2011 ◽  
Vol 127 (4) ◽  
pp. 1515-1520 ◽  
Author(s):  
Joanna Natalia Buffoni ◽  
Ivan Bonizzi ◽  
Alfredo Pauciullo ◽  
Luigi Ramunno ◽  
Maria Feligini

2006 ◽  
Vol 113 (3-4) ◽  
pp. 283-291 ◽  
Author(s):  
Benoît Muylkens ◽  
François Meurens ◽  
Frédéric Schynts ◽  
Katalin de Fays ◽  
Aldo Pourchet ◽  
...  

2002 ◽  
Vol 76 (13) ◽  
pp. 6660-6668 ◽  
Author(s):  
Timothy J. Mahony ◽  
Fiona M. McCarthy ◽  
Jennifer L. Gravel ◽  
Lani West ◽  
Peter L. Young

ABSTRACT The complete genome of bovine herpesvirus 1 (BoHV-1) strain V155 has been cloned as a bacterial artificial chromosome (BAC). Following electroporation into Escherichia coli strain DH10B, the BoHV-1 BAC was stably propagated over multiple generations of its host. BAC DNA recovered from DH10B cells and transfected into bovine cells produced a cytopathic effect which was indistinguishable from that of the parent virus. Analysis of the replication kinetics of the viral progeny indicated that insertion of the BAC vector into the thymidine kinase gene did not affect viral replication. Specific manipulation of the BAC was demonstrated by deleting the gene encoding glycoprotein E by homologous recombination in DH10B cells facilitated by GET recombination. These studies illustrate that the propagation and manipulation of herpesviruses in bacterial systems will allow for rapid and accurate characterization of BoHV-1 genes. In turn, this will allow for the full utilization of BoHV-1 as a vaccine vector.


1986 ◽  
Vol 89 (1-4) ◽  
pp. 57-68 ◽  
Author(s):  
P. A. Rota ◽  
R. K. Maes ◽  
J. F. Evermann

2013 ◽  
Vol 164 (3-4) ◽  
pp. 222-228 ◽  
Author(s):  
Luigi Bertolotti ◽  
Alfonso Rosamilia ◽  
Margherita Profiti ◽  
Emiliana Brocchi ◽  
Loretta Masoero ◽  
...  

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