Development of a multiplex real-time PCR for the simultaneous detection of herpes simplex and varicella zoster viruses in cerebrospinal fluid and lesion swab specimens

2016 ◽  
Vol 229 ◽  
pp. 16-23 ◽  
Author(s):  
Anita A. Wong ◽  
Kanti Pabbaraju ◽  
Sallene Wong ◽  
Raymond Tellier
Keyword(s):  
Cerebrospinal Fluid ◽  
Herpes Simplex ◽  
Real Time ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Varicella Zoster

scholarly journals Detection of Herpes Simplex and Varicella-Zoster Virus in Clinical Specimens by Multiplex Real-Time PCR and Melting Curve Analysis

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Yun Ji Hong ◽  
Mi Suk Lim ◽  
Sang Mee Hwang ◽  
Taek Soo Kim ◽  
Kyoung Un Park ◽  
...  
Keyword(s):  
Herpes Simplex ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Laboratory ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Clinical Specimens ◽  
Varicella Zoster ◽  
Hsv 1

Herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2), and varicella-zoster virus (VZV) are common agents resulting in various forms of clinical manifestation from skin vesicle to disseminated viral infection. The aim of the present study was to develop a real-time PCR and melting curve analysis which detect and differentiate HSV-1, HSV-2, and VZV, to compare with PCR-RFLP using clinical specimens, and to introduce the 4-year experience in the clinical laboratory. Three pairs of primers for HSV-1, HSV-2, and VZV were designed. Primers for human endogenous retrovirus-3 (HERV-3), an internal control, were adopted. A hundred selected specimens and many clinical specimens were tested for methods comparison and assay validation. Increased sensitivity and specificity were obtained from real-time PCR. In review of results of clinical specimens submitted to clinical laboratory, a total of 46 of 3,513 specimens were positive in cerebrospinal fluids, blood, skin vesicles, genital swabs, aqueous humor, and ear discharge. Thus, this method could be a rapid and accurate alternative to virus culture and other molecular tests for detection and typing of HSV-1, HSV-2, and VZV.

Simultaneous detection of herpes simplex virus types 1 and 2 by real-time PCR and Pyrosequencing

2005 ◽  
Vol 33 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Martin E. Adelson ◽  
Melanie Feola ◽  
Jason Trama ◽  
Richard C. Tilton ◽  
Eli Mordechai
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Real Time ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Simplex Virus

Development and Clinical Validation of a Multiplex Real-time PCR Assay for Herpes Simplex and Varicella Zoster Virus

2013 ◽  
Vol 22 (4) ◽  
pp. 245-248 ◽  
Author(s):  
Thean Yen Tan ◽  
Hao Zou ◽  
Danny Chee Tiong Ong ◽  
Khor Jia Ker ◽  
Martin Tze Wei Chio ◽  
...  
Keyword(s):  
Herpes Simplex ◽  
Varicella Zoster Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Validation ◽  
Pcr Assay ◽  
Varicella Zoster
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