Quantitative real time PCR detection of Varicella-zoster virus DNA in cerebrospinal fluid in patients with neurological disease

2004 ◽  
Vol 194 (1-2) ◽  
pp. 7-12 ◽  
Author(s):  
Stephan W. Aberle ◽  
Judith H. Aberle ◽  
Christoph Steininger ◽  
Elisabeth Puchhammer-St�ckl
Keyword(s):  
Cerebrospinal Fluid ◽  
Varicella Zoster Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Neurological Disease ◽  
Pcr Detection ◽  
Quantitative Real Time Pcr ◽  
Varicella Zoster

Diagnosis of neuroschistosomiasis by antibody specificity index and semi-quantitative real-time PCR from cerebrospinal fluid and serum

2014 ◽  
Vol 63 (2) ◽  
pp. 309-312 ◽  
Author(s):  
Georg Härter ◽  
Hagen Frickmann ◽  
Sebastian Zenk ◽  
Dominic Wichmann ◽  
Bettina Ammann ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Symptoms ◽  
Diagnostic Procedures ◽  
Lower Limbs ◽  
Antibody Specificity ◽  
Quantitative Real Time Pcr ◽  
Specific Antibodies ◽  
Routine Diagnosis

We describe the case of a 16-year-old German male expatriate from Ghana who presented with obstipation, dysuria, dysaesthesia of the gluteal region and the lower limbs, bilateral plantar hypaesthesia and paraesthesia without pareses. A serum–cerebrospinal fluid (CSF) Schistosoma spp. specific antibody specificity index of 3.1 was considered highly suggestive of intrathecal synthesis of anti-Schistosoma spp. specific antibodies, although standardization of this procedure has not previously been described. Diagnosis was confirmed by detection of Schistosoma DNA in CSF by semi-quantitative real-time PCR at 100-fold concentration compared with serum. Accordingly the two diagnostic procedures, which have not previously been applied for routine diagnosis, appear to be useful for the diagnosis of neuroschistosomiasis. Clinical symptoms resolved following anthelmintic and anti-inflammatory therapy.

Quantitative real-time PCR detection of insulin signalling-related genes in pancreatic islets isolated from healthy cats

2010 ◽  
Vol 183 (3) ◽  
pp. 287-293 ◽  
Author(s):  
Eric Zini ◽  
Marco Franchini ◽  
Melania Osto ◽  
Andrea Vögtlin ◽  
Franco Guscetti ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Real Time ◽  
Real Time Pcr ◽  
Insulin Signalling ◽  
Pcr Detection ◽  
Quantitative Real Time Pcr

scholarly journals Quantitative Real-Time PCR Detection of TRPV1–4 Gene Expression in Human Leukocytes from Healthy and Hyposensitive Subjects

2008 ◽  
Vol 4 ◽  
pp. 1744-8069-4-51 ◽  
Author(s):  
Giacomo Spinsanti ◽  
Raffaella Zannolli ◽  
Cristina Panti ◽  
Ilaria Ceccarelli ◽  
Letizia Marsili ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Detection ◽  
Quantitative Real Time Pcr ◽  
Human Leukocytes
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