A multiplex reverse transcription PCR assay for simultaneous detection of six main RNA viruses in tomato plants

2019 ◽  
Vol 265 ◽  
pp. 53-58 ◽  
Author(s):  
Huan Liu ◽  
Kuan Wu ◽  
Wei Wu ◽  
Weili Mi ◽  
Xingan Hao ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Rna Viruses ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Tomato Plants ◽  
Reverse Transcription Pcr

scholarly journals Rapid Simultaneous Detection of Enterovirus and Parechovirus RNAs in Clinical Samples by One-Step Real-Time Reverse Transcription-PCR Assay

2011 ◽  
Vol 49 (7) ◽  
pp. 2620-2624 ◽  
Author(s):  
Susan Bennett ◽  
Heli Harvala ◽  
Jeroen Witteveldt ◽  
E. Carol McWilliam Leitch ◽  
Nigel McLeish ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Simultaneous Detection ◽  
Clinical Samples ◽  
Pcr Assay ◽  
Reverse Transcription Pcr ◽  
One Step

scholarly journals Development and Validation of a Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Three Papaya Viruses

Viruses ◽  
2014 ◽  
Vol 6 (10) ◽  
pp. 3893-3906 ◽  
Author(s):  
Decai Tuo ◽  
Wentao Shen ◽  
Yong Yang ◽  
Pu Yan ◽  
Xiaoying Li ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Reverse Transcription Pcr ◽  
Development And Validation

A multiple reverse transcription PCR assay for simultaneous detection of four main viruses in kiwifruit

2020 ◽  
Vol 156 (4) ◽  
pp. 1207-1212 ◽  
Author(s):  
Qiding Peng ◽  
Long Qiu ◽  
Ting Yang ◽  
Jiachen Ning ◽  
Qianyi Xu ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Reverse Transcription Pcr

scholarly journals Simultaneous Detection and Differentiation between Wild-Type and Vaccine Measles Viruses by a Multiplex Real-Time Reverse Transcription-PCR Assay

2019 ◽  
Vol 57 (4) ◽  
Author(s):  
Kanti Pabbaraju ◽  
Kara Gill ◽  
Anita A. Wong ◽  
Graham A. Tipples ◽  
Joanne Hiebert ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Measles Virus ◽  
Respiratory Infections ◽  
Simultaneous Detection ◽  
Contact Tracing ◽  
Wild Type ◽  
Pcr Assay ◽  
Live Attenuated Vaccine ◽  
Reverse Transcription Pcr

ABSTRACT Measles is one of the most contagious viral respiratory infections and was declared to be eliminated from Canada in 1998; however, measles cases and outbreaks still occur every year through reintroduction from other parts of the world. Laboratory confirmation of measles virus (MV) RNA by real-time PCR provides a definitive diagnosis, and molecular analysis to determine the genotype is the only way to distinguish between wild-type and vaccine strains. This distinction is important since live attenuated vaccine strains are able to replicate in the patient and can be associated with rash and fever but are poorly transmissible, if at all. Prompt reporting of measles cases to local authorities, including differentiation between wild-type and vaccine strains, allows for optimal management and contact tracing. The development and validation of a multiplex real-time reverse transcription-PCR (rtRT-PCR) assay for the simultaneous detection and differentiation of the Moraten and Schwarz vaccine strains from presumptive wild-type MV in a format that can be easily implemented for high-throughput testing of patient samples are reported here. This assay is sensitive, specific, reproducible, and 100% accurate in comparison with the gold standard comparator assay.

scholarly journals Development of a multiplex reverse transcription-PCR assay for simultaneous detection of garlic viruses

2015 ◽  
Vol 14 (5) ◽  
pp. 900-908
Author(s):  
Xin-xi HU ◽  
Yan LEI ◽  
Pei WANG ◽  
Lin-fei TANG ◽  
Chang-zheng HE ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Reverse Transcription Pcr

scholarly journals Implementation of an in-house real-time reverse transcription-PCR assay to detect the emerging SARS-CoV-2 N501Y variants

2021 ◽  
pp. 104868
Author(s):  
Marielle BEDOTTO ◽  
Pierre-Edouard FOURNIER ◽  
Linda HOUHAMDI ◽  
Philippe COLSON ◽  
Didier RAOULT
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Pcr Assay ◽  
Reverse Transcription Pcr
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