Differential detection of zika virus based on PCR

2022 ◽  
pp. 114459
Author(s):  
Daniel Alzate ◽  
Esteban Marín ◽  
Jahir Orozco ◽  
Carlos Muskus
Keyword(s):  
Zika Virus ◽  
Differential Detection

Genosensors for differential detection of Zika virus

Talanta ◽  
2020 ◽  
Vol 210 ◽  
pp. 120648 ◽  
Author(s):  
Daniel Alzate ◽  
Sebastian Cajigas ◽  
Sara Robledo ◽  
Carlos Muskus ◽  
Jahir Orozco
Keyword(s):  
Zika Virus ◽  
Differential Detection

Differential Detection of Single-Chain and Two-Chain Urokinase-Type Plasminogen Activator by a New Immunoadsorbent-Amidolytic Assay (IAA)

1986 ◽  
Vol 56 (03) ◽  
pp. 407-410 ◽  
Author(s):  
Angelo Corti ◽  
Maria Luisa Nolli ◽  
Giovanni Cassani
Keyword(s):  
Plasminogen Activator ◽  
Carcinoma Cell ◽  
Differential Detection ◽  
Single Chain ◽  
Response Curves ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator ◽  
Before And After ◽  
Culture Supernatants ◽  
Human Epidermoid Carcinoma

SummaryA new immunoadsorbent-amidolytic assay (IAA) for the specific differential detection of two-chain urokinase-type plasminogen activator (tcu-PA) and its single-chain precursor (scu-PA) in cell culture supernatants has been developed. The assay combines the selectivity of immunoassays with the specificity of enzyme activity assays exploiting both the antigenic and enzymatic properties of the two proteins. tcu-PA and scu-PA are selectively immunoadsorbed on the wells of a microtiterplate coated with the monoclonal antibody 5B4 and tested for enzymatic activity before and after activation by plasmin treatment. Both proteins are determined with similar efficiency since overlapping dose-response curves were obtained in the range between 12.5-200 ng/ml. The assay has been used to determine tcu-PA and scu-PA in A431 human epidermoid carcinoma cell supernatants. The analytical recoveries for tcu-PA and scu-PA added to A431 cell supernatants were 95.2% and 96.9% respectively. The intra- and inter-assay variations (CV) were 5.5% and 9.0% for tcu-PA and 9.7% and 9.8% for scu-PA respectively.

Preventing Transmission of Zika Virus in Labor and Delivery Settings Through Implementation of Standard Precautions — United States, 2016

2016 ◽  
Vol 65 (11) ◽  
Author(s):  
Christine K. Olson ◽  
Martha Iwamoto ◽  
Kiran M. Perkins ◽  
Kara N.D. Polen ◽  
Jeffrey Hageman ◽  
...  
Keyword(s):  
United States ◽  
Zika Virus ◽  
Labor And Delivery ◽  
Standard Precautions

Increase in Reported Prevalence of Microcephaly in Infants Born to Women Living in Areas with Confirmed Zika Virus Transmission During the First Trimester of Pregnancy — Brazil, 2015

2016 ◽  
Vol 65 (9) ◽  
Author(s):  
Wanderson Kleber de Oliveira ◽  
Juan Cortez-Escalante ◽  
Wanessa Tenório Gonçalves Holanda De Oliveira ◽  
Greice Madeleine Ikeda do Carmo ◽  
Cláudio Maierovitch Pessanha Henriques ◽  
...  
Keyword(s):  
Zika Virus ◽  
Virus Transmission ◽  
First Trimester ◽  
First Trimester Of Pregnancy

Increase in Reported Prevalence of Microcephaly in Infants Born to Women Living in Areas with Confirmed Zika Virus Transmission During the First Trimester of Pregnancy — Brazil, 2015

2016 ◽  
Vol 65 (9) ◽  
pp. 242-247 ◽  
Author(s):  
Wanderson Kleber de Oliveira ◽  
Juan Cortez-Escalante ◽  
Wanessa Tenório Gonçalves Holanda De Oliveira ◽  
Greice Madeleine Ikeda do Carmo ◽  
Cláudio Maierovitch Pessanha Henriques ◽  
...  
Keyword(s):  
Zika Virus ◽  
Virus Transmission ◽  
First Trimester ◽  
First Trimester Of Pregnancy

Transmission of Zika Virus Through Sexual Contact with Travelers to Areas of Ongoing Transmission — Continental United States, 2016

2016 ◽  
Vol 65 (8) ◽  
Author(s):  
Susan L. Hills ◽  
Kate Russell ◽  
Morgan Hennessey ◽  
Charnetta Williams ◽  
Alexandra M. Oster ◽  
...  
Keyword(s):  
United States ◽  
Zika Virus ◽  
Sexual Contact

Update: Interim Guidance for Prevention of Sexual Transmission of Zika Virus — United States, 2016

2016 ◽  
Vol 65 (12) ◽  
Author(s):  
Alexandra M. Oster ◽  
Kate Russell ◽  
Jo Ellen Stryker ◽  
Allison Friedman ◽  
Rachel E. Kachur ◽  
...  
Keyword(s):  
United States ◽  
Zika Virus ◽  
Sexual Transmission ◽  
Prevention Of Sexual Transmission

Estimating Contraceptive Needs and Increasing Access to Contraception in Response to the Zika Virus Disease Outbreak — Puerto Rico, 2016

2016 ◽  
Vol 65 (12) ◽  
Author(s):  
Naomi K. Tepper ◽  
Howard I. Goldberg ◽  
Manuel I. Vargas Bernal ◽  
Brenda Rivera ◽  
Meghan T. Frey ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Zika Virus ◽  
Virus Disease ◽  
Disease Outbreak ◽  
Access To Contraception
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