Mechanism of (−)clausenamide induced calcium transient in primary culture of rat cortical neurons

Life Sciences ◽  
2004 ◽  
Vol 74 (11) ◽  
pp. 1427-1434 ◽  
Author(s):  
Kang Tang ◽  
Jun-Tian Zhang
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Calcium Transient ◽  
Rat Cortical Neurons

scholarly journals Effects of ethanol on the desensitization of GABA-induced currents in the primary culture of rat cortical neurons.

1994 ◽  
Vol 64 ◽  
pp. 196
Author(s):  
Shuji Tsujiyama ◽  
Akinori Akaike ◽  
Hisamitsu Ujihara ◽  
Masashi Sasa
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Rat Cortical Neurons ◽  
Induced Currents

Fibroblast growth factor 2 induces apoptosis in the early primary culture of rat cortical neurons

2010 ◽  
Vol 316 (14) ◽  
pp. 2278-2290 ◽  
Author(s):  
Tatsurou Yagami ◽  
Kenkichi Takase ◽  
Yasuhiro Yamamoto ◽  
Keiichi Ueda ◽  
Nobuo Takasu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Primary Culture ◽  
Fibroblast Growth Factor ◽  
Cortical Neurons ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Rat Cortical Neurons ◽  
Early Primary

Mulberroside a protects against ischemic impairment in primary culture of rat cortical neurons after oxygen-glucose deprivation followed by reperfusion

2014 ◽  
Vol 92 (7) ◽  
pp. 944-954 ◽  
Author(s):  
Cai-Ping Wang ◽  
Lu-Zhong Zhang ◽  
Gui-Cai Li ◽  
Yun-wei Shi ◽  
Jian-Long Li ◽  
...  
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Rat Cortical Neurons ◽  
Mulberroside A

scholarly journals The ubiquitylated protein profile of rat cortical neurons is altered in a primary culture model of stroke (728.12)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Michael Dominic Chua ◽  
Bryce Pasqualotto ◽  
Gordon Rintoul ◽  
Julian Guttman
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Protein Profile ◽  
Culture Model ◽  
Rat Cortical Neurons ◽  
Ubiquitylated Protein

Biological activities of laminin-111-derived peptide-chitosan matrices in a primary culture of rat cortical neurons

2018 ◽  
Vol 648 ◽  
pp. 53-59 ◽  
Author(s):  
Hideki Hayashi ◽  
Mariko Yamada ◽  
Jun Kumai ◽  
Norio Takagi ◽  
Motoyoshi Nomizu
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Biological Activities ◽  
Rat Cortical Neurons

scholarly journals Neuroprotective effects of orientin on oxygen-glucose deprivation/reperfusion-induced cell injury in primary culture of rat cortical neurons

2017 ◽  
Vol 243 (1) ◽  
pp. 78-86 ◽  
Author(s):  
Tian Tian ◽  
Junan Zeng ◽  
Guangyu Zhao ◽  
Wenjing Zhao ◽  
Songyi Gao ◽  
...  
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Caspase 3 ◽  
Cell Injury ◽  
Antioxidant Properties ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Ros Generation ◽  
Neuroprotective Effects ◽  
Rat Cortical Neurons

Orientin (luteolin-8-C-glucoside) is a phenolic compound found abundantly in millet, juice, and peel of passion fruit and has been shown to have antioxidant properties. In the present study, we explored the effects of orientin on oxygen-glucose deprivation/reperfusion (OGD/RP)-induced cell injury in primary culture of rat cortical neurons using an in vitro model of neonatal ischemic brain injury. The reduced cell viability and elevated lactate dehydrogenase leakage were observed after OGD/RP exposure, which were then reversed by orientin (10, 20, and 30 µM) pretreatment in a dose-dependent manner. Additionally, OGD/RP treatment resulted in significant oxidative stress, accompanied by enhanced intracellular reactive oxygen species (ROS) generation, and obvious depletion in the activities of intracellular Mn-superoxide dismutase, catalase, and glutathione peroxidase antioxidases. However, these effects were dose dependently restored by orientin pretreatment. We also found that orientin pretreatment dose dependently suppressed [Ca2+]i increase and mitochondrial membrane potential dissipation caused by OGD/RP in primary culture of rat cortical neurons. Western blot analysis showed that OGD/RP exposure induced a distinct decrease of Bcl-2 protein and a marked elevation of Bax, caspase-3, and cleaved caspase-3 proteins; whereas these effects were dose dependently reversed by orientin incubation. Both the caspase-3 activity and the apoptosis rate were increased under OGD/RP treatment, but was then dose dependently down-regulated by orientin (10, 20, and 30 µM) incubation. Moreover, orientin pretreatment dose dependently inhibited OGD/RP-induced phosphorylation of JNK and ERK1/2. Notably, JNK inhibitor SP600125 and ERK1/2 inhibitor PD98059 also dramatically attenuated OGD/RP-induced cell viability loss and ROS generation, and further, orientin failed to protect cortical neurons with the interference of JNK activator anisomycin or ERK1/2 activator FGF-2. Taken together, these results demonstrated that orientin has significant neuroprotective effects against OGD/RP-induced cell injury via JNK and ERK1/2 signaling pathways in primary culture of rat cortical neurons. Impact statement Orientin has been used in traditional eastern medicine and reported to possess antioxidant properties. However, the effects of orientin on neonatal ischemic brain injury and the underlying mechanisms involved have not been studied. Our results showed that orientin exerts significant neuroprotective effects on cell injury caused by oxygen-glucose deprivation/reperfusion via the JNK and ERK1/2 signaling pathways in primary culture of rat cortical neurons, implying the potential therapeutic application of orientin via the suppression of oxidative stress and cell apoptosis. This research suggested that orientin may be used as a therapeutic and preventive option for newborn cerebral ischemia/reperfusion injury.

Epileptiform postsynaptic currents in primary culture of rat cortical neurons: Calcium mechanisms

2014 ◽  
Vol 8 (2) ◽  
pp. 169-177 ◽  
Author(s):  
D. A. Sibarov ◽  
P. A. Abushik ◽  
A. E. Bolshakov ◽  
T. V. Karelina ◽  
I. I. Krivoi ◽  
...  
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Postsynaptic Currents ◽  
Rat Cortical Neurons
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