Neuroprotective effect of schizandrin A on oxygen and glucose deprivation/reperfusion-induced cell injury in primary culture of rat cortical neurons

2014 ◽  
Vol 70 (3) ◽  
pp. 735-747 ◽  
Author(s):  
Cai-Ping Wang ◽  
Gui-Cai Li ◽  
Yun-Wei Shi ◽  
Xiao-Chuan Zhang ◽  
Jian-Long Li ◽  
...  
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Cell Injury ◽  
Neuroprotective Effect ◽  
Glucose Deprivation ◽  
Oxygen And Glucose Deprivation ◽  
Rat Cortical Neurons

scholarly journals Neuroprotective effects of orientin on oxygen-glucose deprivation/reperfusion-induced cell injury in primary culture of rat cortical neurons

2017 ◽  
Vol 243 (1) ◽  
pp. 78-86 ◽  
Author(s):  
Tian Tian ◽  
Junan Zeng ◽  
Guangyu Zhao ◽  
Wenjing Zhao ◽  
Songyi Gao ◽  
...  
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Caspase 3 ◽  
Cell Injury ◽  
Antioxidant Properties ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Ros Generation ◽  
Neuroprotective Effects ◽  
Rat Cortical Neurons

Orientin (luteolin-8-C-glucoside) is a phenolic compound found abundantly in millet, juice, and peel of passion fruit and has been shown to have antioxidant properties. In the present study, we explored the effects of orientin on oxygen-glucose deprivation/reperfusion (OGD/RP)-induced cell injury in primary culture of rat cortical neurons using an in vitro model of neonatal ischemic brain injury. The reduced cell viability and elevated lactate dehydrogenase leakage were observed after OGD/RP exposure, which were then reversed by orientin (10, 20, and 30 µM) pretreatment in a dose-dependent manner. Additionally, OGD/RP treatment resulted in significant oxidative stress, accompanied by enhanced intracellular reactive oxygen species (ROS) generation, and obvious depletion in the activities of intracellular Mn-superoxide dismutase, catalase, and glutathione peroxidase antioxidases. However, these effects were dose dependently restored by orientin pretreatment. We also found that orientin pretreatment dose dependently suppressed [Ca2+]i increase and mitochondrial membrane potential dissipation caused by OGD/RP in primary culture of rat cortical neurons. Western blot analysis showed that OGD/RP exposure induced a distinct decrease of Bcl-2 protein and a marked elevation of Bax, caspase-3, and cleaved caspase-3 proteins; whereas these effects were dose dependently reversed by orientin incubation. Both the caspase-3 activity and the apoptosis rate were increased under OGD/RP treatment, but was then dose dependently down-regulated by orientin (10, 20, and 30 µM) incubation. Moreover, orientin pretreatment dose dependently inhibited OGD/RP-induced phosphorylation of JNK and ERK1/2. Notably, JNK inhibitor SP600125 and ERK1/2 inhibitor PD98059 also dramatically attenuated OGD/RP-induced cell viability loss and ROS generation, and further, orientin failed to protect cortical neurons with the interference of JNK activator anisomycin or ERK1/2 activator FGF-2. Taken together, these results demonstrated that orientin has significant neuroprotective effects against OGD/RP-induced cell injury via JNK and ERK1/2 signaling pathways in primary culture of rat cortical neurons. Impact statement Orientin has been used in traditional eastern medicine and reported to possess antioxidant properties. However, the effects of orientin on neonatal ischemic brain injury and the underlying mechanisms involved have not been studied. Our results showed that orientin exerts significant neuroprotective effects on cell injury caused by oxygen-glucose deprivation/reperfusion via the JNK and ERK1/2 signaling pathways in primary culture of rat cortical neurons, implying the potential therapeutic application of orientin via the suppression of oxidative stress and cell apoptosis. This research suggested that orientin may be used as a therapeutic and preventive option for newborn cerebral ischemia/reperfusion injury.

Apocynum venetum leaf extract protects rat cortical neurons from injury induced by oxygen and glucose deprivation in vitro

2010 ◽  
Vol 88 (9) ◽  
pp. 907-917 ◽  
Author(s):  
Jun Xiang ◽  
Yu-Ping Tang ◽  
Zi-Yi Zhou ◽  
Pin Wu ◽  
Zhong Wang ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Leaf Extract ◽  
Caspase 3 ◽  
Cell Injury ◽  
Glucose Deprivation ◽  
Oxygen And Glucose Deprivation ◽  
Bax Protein ◽  
Rat Cortical Neurons ◽  
Apocynum Venetum

This study aimed to investigate the protective effect of Apocynum venetum leaf extract (AVLE) on an in vitro model of ischemia–reperfusion induced by oxygen and glucose deprivation (OGD) and further explored the possible mechanisms underlying protection. Cell injury was assessed by morphological examination using phase-contrast microscopy and quantified by measuring the amount of lactate dehydrogenase (LDH) leakage; cell viability was measured by XTT reduction. Neuronal apoptosis was determined by flow cytometry, and electron microscopy was used to study morphological changes of neurons. Caspase-3, -8, and -9 activation and Bcl-2/Bax protein expression were determined by Western blot analysis. We report that treatment with AVLE (5 and 50 µg/mL) effectively reduced neuronal cell death and relieved cell injury induced by OGD. Moreover, AVLE decreased the percentage of apoptotic neurons, relieved neuronal morphological damage, suppressed overexpression of active caspase-3 and -8 and Bax, and inhibited the reduction of Bcl-2 expression. These findings indicate that AVLE protects against OGD-induced injury by inhibiting apoptosis in rat cortical neurons by down-regulating caspase-3 activation and modulating the Bcl-2/Bax ratio.

Mulberroside a protects against ischemic impairment in primary culture of rat cortical neurons after oxygen-glucose deprivation followed by reperfusion

2014 ◽  
Vol 92 (7) ◽  
pp. 944-954 ◽  
Author(s):  
Cai-Ping Wang ◽  
Lu-Zhong Zhang ◽  
Gui-Cai Li ◽  
Yun-wei Shi ◽  
Jian-Long Li ◽  
...  
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Rat Cortical Neurons ◽  
Mulberroside A

scholarly journals Neuroprotective effects of arbutin against oxygen and glucose deprivation-induced oxidative stress and neuroinflammation in rat cortical neurons

2021 ◽  
Vol 72 (1) ◽  
pp. 123-134
Author(s):  
Jingjing Tan ◽  
Manoj Kumar Yadav ◽  
Sushma Devi ◽  
Manish Kumar
Keyword(s):  
Cortical Neurons ◽  
Glucose Deprivation ◽  
Cultured Neurons ◽  
Neuroprotective Effects ◽  
Oxygen And Glucose Deprivation ◽  
Rat Cortical Neurons ◽  
Pre Treatment ◽  
Factor Α ◽  
The Brain ◽  
Necrosis Factor

Abstract In this study, the neuroprotective potential of arbutin (100 µmol L−1) pre-treatment and post-treatment against oxygen/ glucose deprivation (OGD) and reoxygenation (R) induced ischemic injury in cultured rat cortical neurons was explored. The OGD (60 min) and reoxygenation (24 h) treatment significantly (p < 0.001) compromised the antioxidant defence in cultured neurons. Subsequently, an increase (p < 0.001) in lipid peroxidation and inflammatory cytokines (tumour necrosis factor-α and nuclear factor kappa-B) declined neuron survival. In pre- and post-condition experiments, treatment with arbutin enhanced both survival (p < 0.01) and integrity (p < 0.05) of cultured neurons. Results showed that arbutin protects (p < 0.05) against peroxidative changes, inflammation, and enhanced the antioxidant activity (e.g., glutathione, superoxide dismutase and catalase) in cultured neurons subjected to OGD/R. It can be inferred that arbutin could protect against ischemic injuries and stroke. The anti-ischemic activity of arbutin can arrest post-stroke damage to the brain.

scholarly journals Inhibition of Glutamate Release via Recovery of ATP Levels Accounts for a Neuroprotective Effect of Aspirin in Rat Cortical Neurons Exposed to Oxygen-Glucose Deprivation

Stroke ◽  
2002 ◽  
Vol 33 (1) ◽  
pp. 261-267 ◽  
Author(s):  
Javier De Cristóbal ◽  
Antonio Cárdenas ◽  
Ignacio Lizasoain ◽  
Juan Carlos Leza ◽  
Paz Fernández-Tomé ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Neuroprotective Effect ◽  
Glutamate Release ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Atp Levels ◽  
Rat Cortical Neurons

scholarly journals Effects of ethanol on the desensitization of GABA-induced currents in the primary culture of rat cortical neurons.

1994 ◽  
Vol 64 ◽  
pp. 196
Author(s):  
Shuji Tsujiyama ◽  
Akinori Akaike ◽  
Hisamitsu Ujihara ◽  
Masashi Sasa
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Rat Cortical Neurons ◽  
Induced Currents
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