A stable isotope technique for measuring production and consumption rates of nitrous oxide in coastal waters

2004 ◽  
Vol 86 (3-4) ◽  
pp. 159-168 ◽  
Author(s):  
Stephen Punshon ◽  
Robert M Moore
2015 ◽  
Vol 73 (3) ◽  
pp. 71-92 ◽  
Author(s):  
Qixing Ji ◽  
Andrew R. Babbin ◽  
Xuefeng Peng ◽  
Jennifer L. Bowen ◽  
Bess B. Ward

Nitrous oxide (N2O) is important to Earth's climate because it is a strong absorber of radiation and an important ozone depletion agent. Increasing anthropogenic nitrogen input into the marine environment, especially to coastal waters, has led to increasing N2O emissions. Identifying the nitrogen compounds that serve as substrates for N2O production in coastal waters reveals important pathways and helps us understand their control by environmental factors. In this study, sediments were collected from a long-term fertilization site in Great Sippewissett Marsh, Falmouth, Massachusetts. The 15N tracer incubation time course experiments were conducted and analyzed for potential N2O production and consumption rates. The two nitrogen substrates of N2O production, ammonium and nitrate, correspond to the two production pathways, nitrification and denitrification, respectively. When measurable nitrate was present, despite ambient high ammonium concentrations, denitrification was the major N2O production pathway. When nitrate was absent, ammonium became the dominant substrate for N2O production, via nitrification and coupled nitrification-denitrification. Net N2O consumption was enhanced under low oxygen and nitrate conditions. N2O production and consumption rates increased with increasing levels of nitrogen fertilization in long-term experimental plots. These results indicate that increasing anthropogenic nitrogen input to salt marshes can stimulate sedimentary N2O production via both nitrification and denitrification, whereas episodic oxygen depletion results in net N2O consumption.


2004 ◽  
Vol 91 (2) ◽  
pp. 287-293 ◽  
Author(s):  
Lena Davidsson ◽  
Ekhard E. Ziegler ◽  
Peter Kastenmayer ◽  
Peter van Dael ◽  
Denis Barclay

Infant formulas based on soyabean protein isolate are often used as an alternative to cows'-based formulas. However, the presence of phytic acid in soya formulas has raised concern about the absorption of trace elements and minerals from these products. The aim of the present study was to evaluate mineral and trace element absorption from regular and dephytinised soya formula in healthy infants. Soyabean protein isolate with a relatively low native content of phytic acid was used for production of a regular soya formula (300 mg phytic acid/kg liquid formula) and an experimental formula was based on dephytinised soya protein isolate (<6 mg phytic acid/kg liquid formula). Using a crossover study design, apparent mineral and trace element absorptions were measured by a stable isotope technique based on 72 h faecal excretion of non-absorbed stable isotopes (Zn, Fe, Cu and Ca) and by the chemical balance technique (Mn, Zn, Cu and Ca) in nine infants (69–191 d old). Fe absorption was also measured by erythrocyte incorporation 14 d after intake. The results from the present study demonstrated that Zn absorption, measured by a stable isotope technique, was significantly greater after dephytinisation (mean value 16·7 v. 22·6 %; P=0·03). No other statistically significant differences between the two formulas were observed. The nutritional benefit of dephytinisation was marginal in the present study. Based on these results, the use of soyabean protein isolate with low native content of phytic acid should be promoted for production of soya formulas and adequate addition of ascorbic acid to enhance Fe absorption should be ensured in the products.


1996 ◽  
Vol 74 (6) ◽  
pp. 1057-1068 ◽  
Author(s):  
Stuart A. Alexander ◽  
Keith A. Hobson ◽  
Cheri L. Gratto-Trevor ◽  
Antony W. Diamond

We used gut-content and stable-isotope techniques to determine diets of shorebirds staging at a prairie wetland complex. Stable-isotope ratios for carbon (13C/12C) and nitrogen (15N/14N) varied greatly within and among prey types and shorebirds, depending on location within the complex. Both dietary techniques suggested that Long-billed Dowitchers (Limnodromus scolopaceus) and Stilt Sandpipers (Calidris himantopus) ate mostly invertebrates, whereas Hudsonian (Limosa haemastica) and Marbled godwits (Limosa fedoa) ate mainly Potamogeton pectinatus tubers. In comparison, the stable-isotope technique indicated that godwits, especially juvenile Marbled Godwits, ate more invertebrates than is indicated by the gut-content analysis. The discrepancies between methods reflect the potential for bias in the application of these techniques. Researchers using stable isotopes to assess migratory shorebird diets should be aware of possible complications arising from isotopic variability within prey types, even over small geographic ranges. High isotopic variability at inland agro-wetland complexes might preclude reliable isotopic assessment of shorebird diets, especially long term. Rhizivory in godwits may be more common than is generally recognized, especially at inland stopovers during autumn migration in both North America and Eurasia, and should be factored into conservation initiatives for these species.


Aquaculture ◽  
2020 ◽  
Vol 529 ◽  
pp. 735635
Author(s):  
Dariano Krummenauer ◽  
Paulo C. Abreu ◽  
Luiz Poersch ◽  
Paula Alice Cruz Paiva Reis ◽  
Sabrina Medeiros Suita ◽  
...  

2014 ◽  
Vol 21 (24) ◽  
pp. 14069-14077 ◽  
Author(s):  
Wenhong Fan ◽  
Jinqian Ren ◽  
Chenguang Wu ◽  
Cheng Tan ◽  
Xiaolong Wang ◽  
...  

2016 ◽  
Vol 36 (1) ◽  
Author(s):  
鹿志创 LU Zhichuang ◽  
田甲申 TIAN Jiashen ◽  
王召会 WANG Zhaohui ◽  
马志强 MA Zhiqiang ◽  
韩家波 HAN Jiabo ◽  
...  

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