In spite of the development and widespread dissemination of the model organic shrimp farming systems, the biological communities in the organic shrimp farming ponds have still very limited. Therefore, we conducted a field survey for macrobenthic communities (MC) of the Tam Giang‘s organic shrimp farming ponds (TGOSFP) located in Tam Giang commune, Nam Can district, Ca Mau province during three seasons in 2015 (March - dry, July - transfer and November - wet season). The results indicated that the MC have characterized by high density and slightly diversity that is a rich natural food sources for shrimp in the TGOSFP. Further more, the present study is a first attempt to use of new indices (AMBI - AZTI’s Marine Biotic Index) on MC for determining the ecological quality status of sediment (EcoQ) in the TGOSFP. The following results were also recorded with an undisturbed and slightly disturbed EcoQ in the TGOSFP and the general EcoQ would likely be improved between three seasons. The success of AMBI for detecting EcoQ in Vietnam is specific to this study, but AMBI was likely to improved, in particular tropical regions.