Tryptophan functionalized framework of nanoporous material as fluorescent sensor for trace detection of Zn2+ ions

2021 ◽  
Vol 259 ◽  
pp. 124088
Author(s):  
Jiahui Hua ◽  
Sumei Liu ◽  
Haohua Ma ◽  
Man Zhou ◽  
Cheng Wang ◽  
...  
The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1227-1235 ◽  
Author(s):  
Xiao-Man Wu ◽  
Jun-Hua Zhang ◽  
Zi-Shuo Feng ◽  
Wen-Xin Chen ◽  
Fei Zhang ◽  
...  

A “turn-off” fluorescent sensor based on glutathione-stabilized copper nanoclusters (GSH-Cu NCs) was prepared for the sensitive detection of RFP via a static quenching strategy caused by the inner filter effect (IFE).


2012 ◽  
Vol 171-172 ◽  
pp. 332-337 ◽  
Author(s):  
Chaolong Tang ◽  
Guowen Meng ◽  
Qing Huang ◽  
Zhulin Huang ◽  
Xinrui Zhang ◽  
...  

Carbon ◽  
2019 ◽  
Vol 149 ◽  
pp. 355-363 ◽  
Author(s):  
Yong Han ◽  
Limei Shi ◽  
Xueli Luo ◽  
Xiumei Chen ◽  
Weixia Yang ◽  
...  

2019 ◽  
Author(s):  
Zacharias Thiel ◽  
Pablo Rivera-Fuentes

Many biomacromolecules are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biological functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a role in detoxification and pro-drug activation. Although nitroreductase activity has been detected in mammalian cells, the subcellular localization of this activity remains incompletely characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools of nitroreductase activity within mitochondria. This probe is activated sequentially by nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>


2019 ◽  
Author(s):  
Zacharias Thiel ◽  
Pablo Rivera-Fuentes

Many biomacromolecules are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biological functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a role in detoxification and pro-drug activation. Although nitroreductase activity has been detected in mammalian cells, the subcellular localization of this activity remains incompletely characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools of nitroreductase activity within mitochondria. This probe is activated sequentially by nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>


2020 ◽  
Vol 24 (20) ◽  
pp. 2378-2382
Author(s):  
Andrea Pappalardo ◽  
Chiara M.A. Gangemi ◽  
Rosa Maria Toscano ◽  
Giuseppe Trusso Sfrazzetto

Real-time sensing of Chemical Warfare Agents (CWAs) is today a crucial topic to prevent the lethal effects of a terroristic chemical attack. For this reason, the development of efficient, selective, sensitive and reversible sensoristic devices, able to detect by optical response ppm levels of these compounds, is strongly required. Here, the synthesis of a new fluorescent sensor based on a salen-uranyl scaffold, functionalized with two bodipy moieties, and its application for the detection of sub-ppm levels of CWAs is reported. Detection properties were evaluated by fluorescence measurements and selectivity tests demonstrated the strong affinity for CWAs.


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