Detection of Enteric Viruses on Strawberries and Raspberries Using Capture by Apolipoprotein H

Human noroviruses (HuNoVs) and the hepatitis A virus (HAV) are the main viral causes of foodborne illness worldwide. These viruses are frequently transmitted via fresh and frozen berries, such as strawberries and raspberries. ISO 15216:1 (2017), currently the preferred method for their detection, involves several steps and is time-consuming. Apolipoprotein H (ApoH) has been shown to have a strong affinity for several microorganisms, including HuNoVs. In this article, we report an ApoH-based method of capturing the HAV and HuNoVs adherent to berries and concentrating them for assay. The limit of detection of both viruses suspended in a buffer was low. On strawberries, the HAV was detected down to 104 genome copies/25 g in 100% of cases and down to 103 genome copies/25 g on raspberries in 50% of cases. This sensitivity was not significantly different from that of the ISO method 15216:1 (2017). HuNoV GII.4 was more difficult to detect using the ApoH method. The ApoH CaptoVIR kit does, nevertheless, appear to be usable in the near future as a single-test, multiple-detection method for viruses on fresh and frozen berries.

DOPO-Modified Cellulose Microsphere: Preparation and Application for Selective Adsorption U(VI) under Acidic Solutions

Effective radioactive wastewater disposal is of great significance to the wide use of nuclear energy. In this work, 4, 4ˊ-[1, 4-phenyl-bis (9, 10-dihydro-9-oxa-10-phosphaphenanthrene-10-yl) dimethyneimino)] diphenol (t-DOPO) was used to modify microcrystalline cellulose microsphere (t-DOPOR) to further enhance it affinity toward U(VI) through radiation method. The t-DOPOR were characterized for structural, morphological, and thermal properties by FTIR, SEM and TGA, which prove that t-DOPO is successfully modified on cellulose. Combination the advantage of cellulose and t-DOPO, t-DOPOR possessed abundant functional group (-OH, -NH and P=O), and exhibited extremely strong affinity toward U(VI) with a maximum adsorption capacity of 51.51 mg/g at pH 3. Particularly, A large distribution, KdU, up to 2.54×104 mL g−1 is found, implying extremely strong affinity toward U(VI) than Ln(III) (La(III), Eu(III), Dy(III), Yb(III)) at the binary system. Dynamic column experiment confirmed that t-DOPOR could separate selectively U(VI) in column experiment. In addition, even in the simulated groundwater trace amount of U(VI) was also eliminated efficiently by t-DOPOR. Lastly, the adsorption mechanism elaborated by XPS analysis was inner-sphere surface complexation between U(VI) and -OH, -NH and P=O groups of t-DOPOR. Overall, the synthesized t-DOPOR may be utilized as a promising adsorbent for separation and remediation of U(VI) from wastewater.

Braiding together student and supervisor aspirations in a struggle to decolonize

In this study, we explore a student-supervisor relationship and the development of relational and reflexive research identities as joint actions towards decolonizing management knowledge and practice. We frame a specific case of PhD supervision through he awa whiria the braided rivers metaphor, which emerges from Māori traditions. This metaphor recognizes a plurality of knowledge streams that can start from different sources, converge, braid and depart again, from the mountains to the sea. In this metaphor, each stream maintains its own autonomy and authority, but knowledge is created at an interface in partnership. We use this framing metaphor to illustrate the tensions between co-creating knowledge with an Indigenous community that a research student has kinship ties with and feels a strong affinity to, and navigating the institutional requirements for a PhD within a UK university. We surface two contributions that open up future possibilities for supervision, research and practice. The first is the use of the metaphor to frame the student-supervisor partnership and strategies for decolonizing management knowledge more broadly. The second is the requirement for relational and reflexive research identities in decolonizing management knowledge.

Weakening affinity of SUS304 asperities to die surface with TiN coating for preventing delayed cracks in deep-drawn cups

SUS304 stainless steel has strong affinity to carbon tool steel surfaces. Therefore, the flow of material in the flange portion during the deep drawing process is retarded, leading to increase in amount of wall thicknening along the cup edge and rising risk for delayed cracks. In this paper, TiN coating is applied to the drawing die surface to weaken the affinity. Under elevated blank holding forces (BHF), the experimental results showed that the crack-free BHF range for the TiN coated and the uncoated dies are 5~10 kN and 12 kN, respectively. The entire BHF range for successful drawn cups formed with the coated die are crack-free. The crack-free BHF magnitude is successfully lowered and the range is enlarged with the coating. Crack-free cups having large elongated height and low amount of wall thickening along the cup edge are formed. The weak affinity is evidenced by the low estimated coefficient of friction (COF) obtained from a FE simulation model based on the Coulomb’s law of friction. In contrast, the estimated COF of the uncoated die is high even at the low BHF due to the strong affinity. Therefore, delayed cracks are observed under BHF range of 7~11 kN. At BHF of 12 kN, wear fragments are formed in the boundary layer as a result of the continuous polishing of the SUS304 asperities by the uncoated die asperities. The COF is sharpyly decreased due to the smooth relative movement of contacting surfaces facilitated by the particles and the formation of cracks is prevented. However, the segments tend to penetrate into the SUS304 surface under excessive BHF of 13kN and above. The relative motions of the segments are prohibited, resulting in the reformation of the cracks.

CMAS and ST3GAL4 Play an Important Role in the Adsorption of Influenza Virus by Affecting the Synthesis of Sialic Acid Receptors

Influenza A viruses (IAVs) initiate infection by attaching Hemagglutinin (HA) on the viral envelope to sialic acid (SA) receptors on the cell surface. Importantly, HA of human IAVs has a higher affinity for α-2,6-linked SA receptors, and avian strains prefer α-2,3-linked SA receptors, whereas swine strains have a strong affinity for both SA receptors. Host gene CMAS and ST3GAL4 were found to be essential for IAV attachment and entry. Loss of CMAS and ST3GAL4 hindered the synthesis of sialic acid receptors, which in turn prevented the adsorption of IAV. Further, the knockout of CMAS had an effect on the adsorption of swine, avian and human IAVs. However, ST3GAL4 knockout prevented the adsorption of swine and avian IAV and the impact on avian IAV was more distinct, whereas it had no effect on the adsorption of human IAV. Collectively, our findings demonstrate that knocking out CMAS and ST3GAL4 negatively regulated IAV replication by inhibiting the synthesis of SA receptors, which also provides new insights into the production of gene-edited animals in the future.

The Retablos of Teabo and Mani: The Evolution of Renaissance Altars in Colonial Yucatán

From the turn to seventeenth through the early eighteenth century, three retablos (altarpieces) were created in Yucatán that relied on a similar Renaissance design. The retablos located in the ex-convents of Mani and Teabo all adopt the Spanish sixteenth-century Renaissance style of the Plateresque. Further, the retablos are connected by the inclusion of caryatid framing devices that establishes a strong affinity among the works. Two of the retablos are located in Mani: the Retablo of San Antonio de Padua and the Retablo of Nuestra Señora de Soledad (or sometimes called the Dolores Retablo). At Teabo is the Retablo de Santa Teresita del Niño Jesús (or Las Ánimas). This paper explores the relationships among the retablos by considering their iconography and their styles to address the retablos’ dates and their current locations. While offering insights about these retablos, this contribution also provides a rich discussion of the thriving artistic industry that was present in Yucatán.

Synthesis and properties of oligonucleotides modified with an N-methylguanidine-bridged nucleic acid (GuNA[Me]) bearing adenine, guanine, or 5-methylcytosine nucleobases

Chemical modifications have been extensively used for therapeutic oligonucleotides because they strongly enhance the stability against nucleases, binding affinity to the targets, and efficacy. We previously reported that oligonucleotides modified with an N-methylguanidine-bridged nucleic acid (GuNA[Me]) bearing the thymine (T) nucleobase show excellent biophysical properties for applications in antisense technology. In this paper, we describe the synthesis of GuNA[Me] phosphoramidites bearing other typical nucleobases including adenine (A), guanine (G), and 5-methylcytosine (mC). The phosphoramidites were successfully incorporated into oligonucleotides following the method previously developed for the GuNA[Me]-T-modified oligonucleotides. The binding affinity of the oligonucleotides modified with GuNA[Me]-A, -G, or -mC toward the complementary single-stranded DNAs or RNAs was systematically evaluated. All of the GuNA[Me]-modified oligonucleotides were found to have a strong affinity for RNAs. These data indicate that GuNA[Me] could be a useful modification for therapeutic antisense oligonucleotides.

Assessing the Mechanism of Fluoxetine-Mediated CYP2D6 Inhibition

Fluoxetine is still one of the most widely used antidepressants in the world. The drug is extensively metabolized by several cytochrome P450 (CYP450) enzymes and subjected to a myriad of CYP450-mediated drug interactions. In a multidrug regimen, preemptive mitigation of drug–drug interactions requires knowledge of fluoxetine actions on these CYP450 enzymes. The major metabolic pathway of fluoxetine leading to the formation of its active metabolite, norfluoxetine, is mediated by CYP2D6. Fluoxetine and norfluoxetine are strong affinity substrates of CYP2D6 and can inhibit, potentially through various mechanisms, the metabolism of other sensitive CYP2D6 substrates. Remarkably, fluoxetine-mediated CYP2D6 inhibition subsides long after fluoxetine first passes through the liver and even remains long after the discontinuation of the drug. Herein, we review pharmacokinetic and pharmacogenetic information to help us understand the mechanisms underlying the prolonged inhibition of CYP2D6 following fluoxetine administration. We propose that long-term inhibition of CYP2D6 is likely a result of competitive inhibition. This is due to strong affinity binding of fluoxetine and norfluoxetine to the enzyme and unbound fluoxetine and norfluoxetine levels circulating in the blood for a long period of time because of their long elimination half-life. Additionally, we describe that fluoxetine is a CYP2C9 substrate and a mechanism-based inhibitor of CYP2C19.