A duplex PCR assay for the simultaneous detection and differentiation of Muscovy duck parvovirus and goose parvovirus

2019 ◽  
Vol 47 ◽  
pp. 101439
Author(s):  
Chunhe Wan ◽  
Longfei Cheng ◽  
Cuiteng Chen ◽  
Rongchang Liu ◽  
Shaohua Shi ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Muscovy Duck ◽  
Duplex Pcr ◽  
Pcr Assay ◽  
Goose Parvovirus ◽  
Muscovy Duck Parvovirus ◽  
Duplex Pcr Assay

scholarly journals Detection of Trypanosoma cruzi and Trypanosoma rangeli Infection by Duplex PCR Assay Based on Telomeric Sequences

2003 ◽  
Vol 10 (5) ◽  
pp. 775-779 ◽  
Author(s):  
Miguel Angel Chiurillo ◽  
Gladys Crisante ◽  
Agustina Rojas ◽  
Andreina Peralta ◽  
Manuel Dias ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Human Subjects ◽  
Simultaneous Detection ◽  
Duplex Pcr ◽  
Trypanosoma Rangeli ◽  
Pcr Assay ◽  
Telomeric Sequences ◽  
Species Specific ◽  
Duplex Pcr Assay ◽  
Reduviid Bugs

ABSTRACT We used the species specificity and repetitious nature of subtelomeric kinetoplastida sequences to generate a duplex PCR assay for the simultaneous detection of Trypanosoma cruzi and Trypanosoma rangeli in experimentally and naturally infected triatomine (Reduviid) bugs and in infected human subjects. The assay was species specific and was capable of detecting 1/20th of T. cruzi and 1/4th of T. rangeli cell equivalents without complementary hybridization. In addition, the PCR-based assay was robust enough for direct application to difficult biological samples such as Reduviid feces or guts and was capable of recognizing all T. cruzi and T. rangeli strains and lineages. Because the assay primers amplify entirely different target sequences, no reaction interference was observed, facilitating future adaptation of this assay to an automated format.

A duplex PCR assay for simultaneous detection and differentiation of Capripoxvirus and Orf virus

2007 ◽  
Vol 21 (4) ◽  
pp. 276-281 ◽  
Author(s):  
Min Zheng ◽  
Qi Liu ◽  
Ningyi Jin ◽  
Jiangang Guo ◽  
Xia Huang ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Orf Virus ◽  
Duplex Pcr ◽  
Pcr Assay ◽  
Duplex Pcr Assay

scholarly journals Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples

2009 ◽  
Vol 104 (1) ◽  
pp. 118-120 ◽  
Author(s):  
Juliana Cristina Marinheiro ◽  
Roberta Braga Sanalios ◽  
Daniela Carvalho dos Santos ◽  
Cristovão Alves da Costa ◽  
Charlotte Marianna Hársi
Keyword(s):  
Respiratory Syncytial Virus ◽  
Duplex Pcr ◽  
Pcr Assay ◽  
Syncytial Virus ◽  
Duplex Pcr Assay

Rapid detection of Yersinia enterocolitica serotype O:3 using a duplex PCR assay

2018 ◽  
Vol 154 ◽  
pp. 107-111 ◽  
Author(s):  
Leonardo Alves Rusak ◽  
Rodrigo de Castro Lisboa Pereira ◽  
Isabelle Geoffroy Freitag ◽  
Cristina Barroso Hofer ◽  
Ernesto Hofer ◽  
...  
Keyword(s):  
Yersinia Enterocolitica ◽  
Rapid Detection ◽  
Duplex Pcr ◽  
Pcr Assay ◽  
Serotype O ◽  
Duplex Pcr Assay

A duplex PCR assay for authentication of Ocimum basilicum L. and Ocimum tenuiflorum L in Tulsi churna

Food Control ◽  
2021 ◽  
pp. 108790
Author(s):  
Tasnim Travadi ◽  
Sonal Sharma ◽  
Ramesh Pandit ◽  
Mital Nakrani ◽  
Chaitanya Joshi ◽  
...  
Keyword(s):  
Ocimum Basilicum ◽  
Duplex Pcr ◽  
Pcr Assay ◽  
Ocimum Tenuiflorum ◽  
Duplex Pcr Assay

scholarly journals Development and characterization of a duplex PCR assay in Chinese sturgeon (Acipenser sinensis) for genetic analysis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yacheng Hu ◽  
Xueqing Liu ◽  
Jing Yang ◽  
Kan Xiao ◽  
Binzhong Wang ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Duplex Pcr ◽  
Chinese Sturgeon ◽  
Pcr Assay ◽  
Acipenser Sinensis ◽  
Duplex Pcr Assay
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