In silico design of an immunogen against Acinetobacter baumannii based on a novel model for native structure of Outer membrane protein A

2017 ◽  
Vol 105 ◽  
pp. 201-210 ◽  
Author(s):  
Abolfazl Jahangiri ◽  
Iraj Rasooli ◽  
Parviz Owlia ◽  
Abbas Ali Imani Fooladi ◽  
Jafar Salimian
Keyword(s):  
Membrane Protein ◽  
Acinetobacter Baumannii ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
In Silico ◽  
Protein A ◽  
Native Structure ◽  
In Silico Design ◽  
Outer Membrane Protein A ◽  
Novel Model

scholarly journals Acinetobacter baumannii invades epithelial cells and outer membrane protein A mediates interactions with epithelial cells

2008 ◽  
Vol 8 (1) ◽  
pp. 216 ◽  
Author(s):  
Chul Hee Choi ◽  
Jun Sik Lee ◽  
Yoo Chul Lee ◽  
Tae In Park ◽  
Je Chul Lee
Keyword(s):  
Epithelial Cells ◽  
Membrane Protein ◽  
Acinetobacter Baumannii ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Protein A ◽  
Outer Membrane Protein A

scholarly journals Evaluation of the New Outer Membrane Protein A Epitope-based Vaccines for Mice Model of Acinetobacter baumannii Associated Pneumonia and Sepsis Infection

2021 ◽  
Author(s):  
Kobra Mehdinejadiani ◽  
Ali Hashemi ◽  
Mojgan Bandehpour ◽  
Hoda Rahmani ◽  
Mohammad Mehdi Ranjbar ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Acinetobacter Baumannii ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Protein A ◽  
Survival Rates ◽  
The Other ◽  
Dependent Manner ◽  
Protective Function ◽  
Outer Membrane Protein A

Nosocomial infections caused by Acinetobacter baumannii (A. baumannii) are considered as a global serious problem in hospitalized patients because of emerging antibiotic resistance. Immunotherapy approaches are promising to prevent such infections. In our previous study, five antigenic epitopes of outer membrane protein A (OmpA), as the most dangerous virulence molecule in A. baumanii, were predicted in silico. In this study, the investigators evaluated some immunological aspects of the peptides.Five peptides were separately injected into C5BL/6 mice; then the cytokine production (interleukin-4 and interferon-gamma) of splenocytes and opsonophagocytic activity of immunized serum were assessed. To identify the protective function of the peptides, animal models of sepsis and pneumonia infections were actively and passively immunized with selected peptides and pooled sera of immunized mice, respectively. Then, their survival rates were compared with the non-infected controls.Based on the results, activated spleen cells in P127 peptide-immunized mice exhibited an increased level of IFN-γ compared with the other experimental groups, but not about the IL-4 concentration. The results of the opsonophagocytic assay revealed an appropriate killing the activity of produced antibodies against A. baumannii in a dose-dependent manner. Further, the survival rates of the mice under passive immunization with the immunized sera or active immunization with P127 peptide were significantly more than those in the control group. Moreover, the survival rate of the P127 peptide immunized group was considerably higher than that of the other peptide-immunized group.In conclusion, findings indicated that peptides derived from OmpA can be used as a promising tool for designing the epitope-based vaccines against infections caused by A. baumannii.   

scholarly journals In Silico Design and Evaluation of Acinetobacter baumannii Outer Membrane Protein A (OmpA) Antigenic Peptides as Vaccine Candidate in Immunized Mice

2020 ◽  
Author(s):  
Kobra Mehdinejadiani ◽  
Mojgan Bandehpour ◽  
Ali Hashemi ◽  
Mohammad Mehdi Ranjbar ◽  
Sodabeh Taheri ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Acinetobacter Baumannii ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Protein A ◽  
Epitope Prediction ◽  
Effective Vaccine ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigenic Peptides ◽  
Outer Membrane Protein A

Acinetobacter baumannii is a Gram-negative bacterium that has recently been identified as a leading nosocomial pathogen. Infections by this pathogen result in significant mortality due to antibiotic resistance. An effective vaccine would help alleviate the burden of disease incurred by this pathogen; however, there are currently no licensed vaccines offering protection against Acinetobacter baumannii infection. In this study, considering the fact that outer membrane protein A is one of the most promising vaccine candidates, we predicted T cell and B cell epitopes on this protein using sequence-based epitope prediction tools and determined whether or not mice immunized with these peptides induce an immune response. We selected consensus epitopes including five peptides in different tools with the highest score. 48 female C5BL/6 SPF injected subcutaneously with the peptides (peptide1 to peptide 5 separately) in 100 μL of the solution and sham groups received adjuvant and PBS alone on the same schedule: on day 0 (primary dose) and two booster doses were administered on days 14 and 28. At the end of time, animals euthanized by Isoflurane, and collected sera for assessment of specific antibodies against each peptide by ELISA (Enzyme-linked immunosorbent assay). Immunization of mice showed one of the novel synthetic peptides (peptide 1 (24-50 amino acids)) elicited immune responses. We conclude to combine theoretical methods of epitope prediction and evaluating the potential of immunogenicity for developing vaccines is important.

Sign in / Sign up

Export Citation Format

Share Document