Molecular detection of Puccinia horiana in Chrysanthemum x morifolium through conventional and real-time PCR

2009 ◽  
Vol 76 (2) ◽  
pp. 136-145 ◽  
Author(s):  
Hossein Alaei ◽  
Steve Baeyen ◽  
Martine Maes ◽  
Monica Höfte ◽  
Kurt Heungens
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Molecular Detection ◽  
Chrysanthemum X Morifolium ◽  
Puccinia Horiana

scholarly journals Evaluation of the New BD Max GC Real-Time PCR Assay, Analytically and Clinically as a Supplementary Test for the BD ProbeTec GC Qx Amplified DNA Assay, for Molecular Detection of Neisseria gonorrhoeae

2015 ◽  
Vol 53 (12) ◽  
pp. 3935-3937 ◽  
Author(s):  
Daniel Golparian ◽  
Stina Boräng ◽  
Martin Sundqvist ◽  
Magnus Unemo
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Real Time ◽  
Sensitivity And Specificity ◽  
Real Time Pcr ◽  
Molecular Detection ◽  
Analytical Sensitivity ◽  
Pcr Assay ◽  
Content Type ◽  
Dna Assay ◽  
Supplementary Test

The new BD Max GC real-time PCR assay showed high clinical and analytical sensitivity and specificity. It can be an effective and accurate supplementary test for the BD ProbeTec GC Qx amplified DNA assay, which had suboptimal specificity, and might also be used for initial detection ofNeisseria gonorrhoeae.

scholarly journals Serologic and Molecular Detection of Mycoplasma gallisepticum in Layer Flocks in South Marmara Region of Turkey

2021 ◽  
pp. 29-37
Author(s):  
Elçin Günaydın ◽  
Özlem Kardoğan ◽  
Gülşen Goncagül ◽  
Yavuz Çokal
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Molecular Detection ◽  
Preventive Measures ◽  
Mycoplasma Gallisepticum ◽  
Marmara Region ◽  
Time Loss ◽  
Serological Tests ◽  
Primary Screening ◽  
Pooled Samples

Background: Due to the economic impacts of Mycoplasma gallisepticum (MG) infection in poultry, it is essential to have a fast, reliable and accurate diagnostic test to diagnose the infection. Aims: It was aimed to examine the presence of MG in the South Marmara Region of Turkey where extensive commercial layer flocks exist by RPA, ELISA and real-time PCR. Materials and Methods: In the study, 981 sera and 160 tracheal swab samples (20 swabs per each flock) obtained from eight layer flocks were examined for the presence of MG-antibody by RPA, ELISA, and the presence of MG by real-time PCR, respectively. Results: MG-seropositive flock rate was determined to be 100% by RPA. Twenty-three of the RPA positive sera in each flock LA, LB, LC, LD, LF, LG, and 17 RPA positive sera in flock LE (due to 17 positive RPA sera obtained) were examined for the presence of MG antibody by ELISA, and MG-seropositive flock rate was determined to be 87.5%. As a result of the examination of a total of 32 tracheal swab samples (20 swabs perflock/5 swabs=4 pooled samples, 8 flocksX4 pooled samples= 32 samples) for the presence of MG, real-time PCR positive flock rate was found to be 75%. Conclusion: To decide the flock whether it is infected or not and the initiate effective preventive measures against MG infection as soon as possible; serology should be applied simultaneously with bacteriology and/or PCR to prevent time loss due to shortcomings of serological tests used as primary screening test such as cross reactions, sensitivity and specificity problems.

Rapid molecular detection of extended-spectrum β-lactamase gene variants with a novel ligation-mediated real-time PCR

2012 ◽  
Vol 61 (11) ◽  
pp. 1563-1567 ◽  
Author(s):  
Roel Nijhuis ◽  
Anton van Zwet ◽  
James Cohen Stuart ◽  
Thijs Weijers ◽  
Paul Savelkoul
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Molecular Detection ◽  
Gene Variants ◽  
Extended Spectrum ◽  
Lactamase Gene

scholarly journals On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

10.3791/56891 ◽  
2018 ◽  
Author(s):  
Joseph B. DeShields ◽  
Rachel A. Bomberger ◽  
James W. Woodhall ◽  
David L. Wheeler ◽  
Natalia Moroz ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Molecular Detection

scholarly journals Improved molecular detection of Babesia infections in animals using a novel quantitative real-time PCR diagnostic assay targeting mitochondrial DNA

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Barbara A. Qurollo ◽  
Nikole R. Archer ◽  
Megan E. Schreeg ◽  
Henry S. Marr ◽  
Adam J. Birkenheuer ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Real Time Pcr ◽  
Molecular Detection ◽  
Diagnostic Assay ◽  
Quantitative Real Time Pcr

scholarly journals Molecular Detection of Plasmodium knowlesi in a Dutch Traveler by Real-Time PCR

2012 ◽  
Vol 50 (7) ◽  
pp. 2523-2524 ◽  
Author(s):  
L. Link ◽  
A. Bart ◽  
N. Verhaar ◽  
T. van Gool ◽  
M. Pronk ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Molecular Detection ◽  
Plasmodium Knowlesi
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